Effect of colchicine on analgesic effects of morphine and DADL in rats

Motin, V. G.

doi:10.1007/bf00840090

Cited by 2 publications

(2 citation statements)

References 6 publications

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“…colchicine administration before morphine injection (i.c.v.) prevented the analgesic effect of morphine using tail-flick test 28 . Since, many investigators use colchicine to block microtubular transport of neurotransmitters including endogenous opioids 29 , therefore the hypothesis can be made that via different than opioid system the observed analgesic effect shown by 6 is produced.…”

Section: Discussionmentioning

confidence: 99%

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Cytotoxic, analgesic and anti-inflammatory activity of colchicine and its C-10 sulfur containing derivatives

Kurek

Myszkowski

Okulicz-Kozaryn

et al. 2021

Sci Rep

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Abstract10-Alkylthiocolchicines have been obtained and characterized by spectroscopic methods and their biological activities as: cytotoxic, anti-inflammatory and analgesic activities have been tested. Cytotoxic activity against SKOV-3 ovarian cell line for 10-alkylthiocolchicine analogues was reported and tested compounds showed to be more active than commonly used doxorubicin. Some of tested C-10 alkylthiolated colchicines have been found to exhibit cytotoxicity at levels comparable to that of the natural product—colchicine. 10-Methylthiocolchicine has IC50 = 8 nM and 10-ethylthiocolchicine has IC50 = 47 nM in comparison to colchicine IC50 = 37 nM. Moreover for 10-alkylthioderivatives apoptosis test, cyclin B1 and cell cycle tests were performed. 10-n-Butylthiocolchicine was tested for anti-inflammatory and analgesic activities it showed to produce analgesic rather than anti-inflammatory effect.

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Section: Discussionmentioning

confidence: 99%

“…Cells were differentiated into one from three categories: viable, apoptotic and necrotic/late apoptotic population. The results are presented as mean values ± SD from two experiments 37 .…”

Section: Methodsmentioning

confidence: 99%

Cytotoxic, analgesic and anti-inflammatory activity of colchicine and its C-10 sulfur containing derivatives

Kurek

Myszkowski

Okulicz-Kozaryn

et al. 2021

Sci Rep

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Proteomic analysis of spinal protein expression in rats exposed to repeated intrathecal morphine injection

Shui

Wang

et al. 2007

Proteomics

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Repeated administration of morphine for treating severe chronic pain may lead to neuroadaptive changes in the spinal cord that are thought to underlie molecular mechanisms of the development of morphine tolerance and physical dependence. Here, we employed a 2-D gel-based proteomic technique to detect the global changes of the spinal cord protein expression in rats that had developed morphine tolerance. Morphine tolerance at the spinal cord level was induced by repeated intrathecal injections of morphine (20 microg/10 microL) twice daily for 5 days and evaluated by measurements of paw withdrawal latencies and maximal possible analgesic effect at day 5. After behavioral tests, the lumbar enlargement segments of spinal cord were harvested and proteins resolved by 2-DE. We found that eight proteins were significantly up-regulated or down-regulated in spinal cord after morphine tolerance development, including proteins involved in targeting and trafficking of the glutamate receptors and opioid receptors, proteins involved in oxidative stress, and cytoskeletal proteins, some of which were confirmed by Western blot analysis. Morphine-induced expressional changes of these proteins in the spinal cord might be involved in the central mechanisms that underlie the development of morphine tolerance. It is very likely that these identified proteins may serve as potential molecular targets for prevention of the development of morphine tolerance and physical dependence.

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Effect of colchicine on analgesic effects of morphine and DADL in rats

Cited by 2 publications

References 6 publications

Cytotoxic, analgesic and anti-inflammatory activity of colchicine and its C-10 sulfur containing derivatives

Cytotoxic, analgesic and anti-inflammatory activity of colchicine and its C-10 sulfur containing derivatives

Proteomic analysis of spinal protein expression in rats exposed to repeated intrathecal morphine injection

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