Effect of Colchicine and Oryzalin Treatments on Tetraploid Production of Nucellar Embryos in Meiwa Kumquat (Fortunella crassifolia Swingle)

Yoshio, Masaki; Kashihara, Yukiko; Kurogi, Hironori; Kunitake, Hisato; Komatsu, Hirokazu

doi:10.2503/hrj.3.11

Cited by 10 publications

(2 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been reported that when axillary buds, seeds, and callus have been treated with antimitotic agents, including colchicine and oryzalin, many cytochimeras appeared in fruit crops such as Actinidia deliciosa, Citrus spp., Fortunella crassifolia Swingle, Musa acuminata, Pyrus pyrifolia, and Vitis vinifera L. (Chalak and Legave, 1996;Kadota and Niimi, 2002;Notsuka et al, 2000;Van Duren et al, 1996;Wu and Mooney, 2002;Yahata et al, 2004). Notsuka et al (2000) reported that complete tetraploids were obtained from cytochimeras of 2X+4X by stem cutting in Vitis vinifera.…”

Section: Resultsmentioning

confidence: 99%

Production of a Doubled Haploid from a Haploid Pummelo Using Colchicine Treatment of Axillary Shoot Buds

Yoshio¹,

Kunitake²,

Yabuya³

et al. 2005

jashs

Self Cite

View full text Add to dashboard Cite

To produce the homozygous strain of a haploid plant derived from small seed-derived seedlings of `Banpeiyu' pummelo (Citrus grandis Osbeck), we carried out colchicine treatment to axillary shoot buds of the haploid. Many shoots with cytochimeras (X+2X and 2X+4X) arose from the colchicine-treated axillary buds. When cytochimeric buds of 2X+4X were top-grafted onto trifoliate orange [Poncirus trifoliata (L.) Raf.], a complete diploid shoot with 18 chromosomes was obtained from the cytochimera. This diploid strain showed vigorous growth compared with the original haploid. The leaf weight per unit area and the stomata size in this diploid were significantly larger than those of the original haploid plant, and were almost equal to those of `Banpeiyu' pummelo. The diploid strain was confirmed to be a doubled haploid of a haploid from `Banpeiyu' pummelo, based on random amplified polymorphic DNA (RAPD) analysis and chromosome composition analysis by chromomycin A3 (CMA) staining.

show abstract

Section: Resultsmentioning

confidence: 99%

Production of a Doubled Haploid from a Haploid Pummelo Using Colchicine Treatment of Axillary Shoot Buds

Yoshio¹,

Kunitake²,

Yabuya³

et al. 2005

jashs

Self Cite

View full text Add to dashboard Cite

show abstract

“…Yahata et al [13] produced many tetraploid Meiwa kumquats by applying a colchicine treatment to nucellar embryos. After they were grafted onto the trifoliate oranges [Poncirus trifoliata (L.) Raf.…”

Section: Introductionmentioning

confidence: 99%

The Confirmation of a Ploidy Periclinal Chimera of the Meiwa Kumquat (Fortunella crassifolia Swingle) Induced by Colchicine Treatment to Nucellar Embryos and Its Morphological Characteristics

et al. 2019

Self Cite

View full text Add to dashboard Cite

A ploidy chimera of the Meiwa kumquat (Fortunella crassifolia Swingle), which had been induced by treating the nucellar embryos with colchicine, and had diploid (2n = 2x = 18) and tetraploid (2n = 4x = 36) cells, was examined for its ploidy level, morphological characteristics, and sizes of its cells in its leaves, flowers, and fruits to reveal the ploidy level of each histogenic layer. Furthermore, the chimera was crossed with the diploid kumquat to evaluate the ploidy level of its reproductive organs. The morphological characteristics and the sizes of the cells in the leaves, flowers, and fruits of the chimera were similar to those of the tetraploid Meiwa kumquat and the ploidy periclinal chimera known as “Yubeni,” with diploids in the histogenic layer I (L1) and tetraploids in the histogenic layer II (L2) and III (L3). However, the epidermis derived from the L1 of the chimera showed the same result as the diploid Meiwa kumquat in all organs and cells. The sexual organs derived from the L2 of the chimera were significantly larger than those of the diploid. Moreover, the ploidy level of the seedlings obtained from the chimera was mostly tetraploid. In the midrib derived from the L3, the chimera displayed the fluorescence intensity of a tetraploid by flow cytometric analysis and had the same size of the cells as the tetraploid and the Yubeni. According to these results, the chimera is thought to be a ploidy periclinal chimera with diploid cells in the outermost layer (L1) and tetraploid cells in the inner layers (L2 and L3) of the shoot apical meristem. The chimera had desirable fruit traits for a kumquat such as a thick pericarp, a high sugar content, and a small number of developed seeds. Furthermore, triploid progenies were obtained from reciprocal crosses between the chimera and diploid kumquat.

show abstract

A tetraploid-dominated cytochimera developed from a natural bud mutant of the nonapomictic mandarin variety ‘Orah’

Dang,

Li,

Sun

et al. 2024

Mol Breeding

View full text Add to dashboard Cite

Effect of Colchicine and Oryzalin Treatments on Tetraploid Production of Nucellar Embryos in Meiwa Kumquat (Fortunella crassifolia Swingle)

Cited by 10 publications

References 15 publications

Production of a Doubled Haploid from a Haploid Pummelo Using Colchicine Treatment of Axillary Shoot Buds

Production of a Doubled Haploid from a Haploid Pummelo Using Colchicine Treatment of Axillary Shoot Buds

The Confirmation of a Ploidy Periclinal Chimera of the Meiwa Kumquat (Fortunella crassifolia Swingle) Induced by Colchicine Treatment to Nucellar Embryos and Its Morphological Characteristics

A tetraploid-dominated cytochimera developed from a natural bud mutant of the nonapomictic mandarin variety ‘Orah’

Contact Info

Product

Resources

About