Effect of coconut water and growth regulator supplements on in vitro propagation of Corylus avellana L

Abstract

a b s t r a c tCorylus avellana L. represents an economically important crop in the European Union. Several protocols of hazelnut micropropagation have been tested but have not yet provided effective methods to allow largescale propagation for commercial purposes, due to poor proliferation and yield. The aim of this work was to study the in vitro effects of coconut water (CW) in combinations with gibberellins and cytokinins on proliferation and growth of hazelnut in vitro and the effect of explants orientation on shoot growth. Single axillary buds from mature trees of 'Tonda Gentile delle Langhe' cultivar collected from suckers and crown were cultured on a modified DKW semi-solid basal medium enriched with coconut water, BAP, IAA and GA 3 in several combinations. The addition of 20% coconut water increased the number of adventitious shoots per explant and the combination of 2 mg/L BAP, 0.01 mg/L IAA and 0.5 mg/L GA 3 promoted multiplication and shoots elongation. Shoot multiplication and growth were also affected by explant orientation. The highest rates occurred with sucker explants in the horizontal position.

Organic Compounds: Contents and Their Role in Improving Seed Germination and Protocorm Development in Orchids

“…e development and regeneration of in vitro cultured plant tissues can be improved by adding a variety of organic nutrients [19][20][21]. ese may include coconut water (CW), peptone (P), potato homogenate (PH), banana homogenate (BH), chitosan (CHT), tomato juice (TJ), and yeast extract (YE).…”

Embryogenic Callus Induction from Leaf Tip Explants and Protocorm-Like Body Formation and Shoot Proliferation of Dimorphorchis lowii: Borneon Endemic Orchid

“…After 120 days of culture, 15 % (v/v) coconut water produced a significantly higher percentage of PLBs forming shoot at 70.0 % ± 42.0 with 8.9±2.9 shoots and 5.5±2.6 leaves ( Figure 2C 62.0 ± 13.5 a 62.0 ± 13.5 a 9.9 ± 2.9 b 46.0 ± 20.1 a 3.1 ± 1.9 b 1.7 ± 0.4 b 15 % CW 72.0 ± 11.9 a 72.0 ± 11.9 a 16.9 ± 6.5 a 70.0 ± 42.0 a 8.9 ± 2.9 a 5.5 ± 2.6 a Remarks: Mean values within a column followed by the same letters are not significantly different at p<0.05 according to Duncan's Multiple Range Test; * Data collected after 60 days of culture; ** Data collected after 120 days of culture Overall, coconut water at 15 % (v/v) showed the superiority compared to the other two complex additives (peptone & yeast extract). Coconut water is extensively used in the plant micropropagation due to its growth enhancer substance and cytokinin-type action that assist cell division and stimulate fast growth (Arditti, 2008;Yong, Ge, Ng, & Tan, 2009;Prando, Chiavazza, Faggio, & Contessa, 2014). Coconut water contains high levels of Z-type cytokinin (trans-zeatin riboside, trans-zeatin O-glucoside, dihydrozeatin O-glucoside, trans-zeatin, dihydrozeatin, trans-zeatin riboside-5 -monophosphate), iP-type cytokinin (N6-isopentenyladenine), kinetin, kinetin riboside and ortho-topolin in addition to sugars, vitamins, minerals and amino acids (Yong, Ge, Ng, & Tan, 2009;Tan, Cheng, Bhat, Rusul, & Easa, 2014).…”

The response of growth two clone of tea (Camellia sinensis) with coconut water application after centering

“…Aplikasi air kelapa pada beberapa tanaman sayuran dengan konsentrasi 100%, 75% dan 25% yang dikombinasikan dengan pupuk anorganik, memiliki hasil yang lebih tinggi dibandingkan tanpa pemberian air kelapa (Omo, 2013). Prando et al (2014), menyatakan adanya efek sinergis antara proses pertumbuhan tanaman dan air kelapa dalam meningkatkan pemanjangan tunas.…”