Effect of Coconut Endosperm and Other Growth Stimulants upon the Development In vitro of Embryos of Cocos nucifera

Cutter, Victor M.; Wilson, K. S.

doi:10.1086/335819

Cited by 41 publications

(9 citation statements)

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“…The mature zygotic embryos explants showed better callusing and embryo development, as compared to immature inflorescence which was reported by various authors (Hornung, 1995). Dormancy was also experienced in some embryos and other studies by (Cutter Jr and Wilson, 1954), dormancy was attributed to Means (± SE) followed by the same alphabets in each column were not significantly different at P≤0.05 using Fisher's test n=36. the use of nuts with mature embryos and endosperm which are likely to contain inhibitory substances leading to dormant cells.…”

Section: Discussionmentioning

confidence: 70%

“…Somatic embryogenesis enables mass production of plants all year round and can be used for genetic improvement of all plants irrespective of the species (Bhansali, 1990). Many trials have been attempted since 1954 by different authors to propagate C. nucifera in vitro (Cutter Jr and Wilson, 1954), but still, none of the protocols was found to be efficient. This is because coconut is one of the recalcitrant species to tissue culture and the existing protocols at times cannot be repeated (Solís-Ramos et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

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In vitro regeneration of coconut (Cocos nucifera L) through indirect somatic embryogenesis in Kenya

Bett¹,

Mweu²,

Nyende³

2019

Afr. J. Biotechnol.

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Regeneration of the coconut (Cocos nucifera L) through indirect somatic embryogenesis using 9 to 12 months embryos explants was established in Y3 medium supplemented with 100 to 250 µM 2,4dichlorophenoxyacetic acid (2, 4-D) alone and in combination with gibberellic acid (GA 3), 6benzylaminopurine (BAP) and thiadiazuron (TDZ). The highest callus induction (100%) was observed in medium containing 150 and 250 µM 2, 4-D + 5 µM BAP while the least was in 2, 4-D alone (16.7%) and in combination with 0.35 µM GA 3 (0). The highest embryogenic calli were observed in medium with 125 µM 2, 4-D + 5 µM BAP (100%) and the least was in medium with 2, 4-D alone (16.7) and in combination with 0.35 µM GA 3 (0). Multiple shoot induction was observed in medium supplemented with 10 µM kinetin, 10 µM BAP, 0.5 µM GA3, and 200 µM NAA. Maximum shoot elongation (1.667 cm) was in medium containing 10 µM BAP and the least (0.811 cm) was in medium with 5 µM BAP. Rooting was done in Y3 medium containing three levels of indole-3-butyric acid (IBA). The highest response was observed in Y3 medium supplemented with 5 µM IBA+ 0.5 µM GA 3 both with respect to the number of roots 8.33 and root length 5.10 cm while the least was with 10 µM IBA+ 0.5 µM GA 3 with regard to the number of roots 3.33 and root length 2.83 cm. Acclimatization was achieved in media prepared with soil: sand: manure ratio (3:1:1) with 25% survival rate and vermiculate medium with 8.3% survival rate. Hence, in vitro regeneration of coconut through somatic embryogenesis is a viable alternative for mass propagation.

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Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

In vitro regeneration of coconut (Cocos nucifera L) through indirect somatic embryogenesis in Kenya

Bett¹,

Mweu²,

Nyende³

2019

Afr. J. Biotechnol.

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“…Even endosperm tissue maintained in culture for long periods (Pieczur, 1952;Norstog, 1956b) and the lemnas and paleas of florets (RCdei & RCdei, 1955a, b;La Croix, Naylor & Larter, 1962;La Croix & Canvin, 1963) contain substances which will induce growth in cultured or intact proembryos of certain cereals. Although milk from either young or mature coconuts was equally effective in the experiments described above, paradoxically enough, milk from mature coconuts markedly inhibited the growth of mature coconut embryos (Cutter & Wilson, 1954). The best growth in vitro was obtained in liquid endosperm from young coconuts.…”

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confidence: 86%

Nutrition, Growth and Morphogenesis of Plant Embryos

Raghavan

1966

Biological Reviews

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“…De Guzman, in 1971 reported successful plant development from embryos of the makapuno type of coconut (homozygous recessive embryo which does not grow HI planta). The first attempts of embryo culture of non-makapuno type of coconut were those of Cutter & Wilson (1954) and Abraham & Thomas (1962) Fisher & Tsai in 1978 also reported growth in cultured embryos but complete plant regeneration has not been very successful. Iyer (1981) reported the development of seedlings from embryos of West Coast Tall coconuts but these had a high rate of mortality on transplanting to soil.…”

Section: Introductionmentioning

confidence: 99%

A Report on the Culture of Embryos of Dwarf Coconut, <i>Cocos nucifera</i> L var <i>nana In vitro</i>

Karunaratne

Kurukulaarachchi

Gamage

2009

COCOS

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The possibility of culturing excised embryos of coconut, Cocos nucifera L. var. nana in vitro has been investigated. Embryos were excised from nuts harvested 10-11 months after pollination. Incubation of these embryos in a liquid medium (a modification of Y3 medium, Eeuwens, 1978) at 30-32°C and under a 12hr photoperiod of low light intensity (2.0/iEm" 2 s _1) resulted in about 62/ of the cultured embryos developing into seedlings. A high concentration of sucrose stimulated haustorium development and subsequent root initiation. A significant retardation in growth of the embryos and complete cessation of haustorium development and root initiation occured in the absence of activated charcoal. Four month old seedlings were transplanted successfully into soil. It is concluded that activated charcoal and a high concentration of sucrose are two important factors contribu ting to successful growth of coconut embryos in vitro. Attempts were also made to preserve the coconut embryos in culture in a dormant state. A special survival medium which suppresses the growth of the embryos has been developed. 32 '/. of the embryos incubated in the survival medium for a period of 5 months developed into seedlings when transferred to the development medium.

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Effect of Coconut Endosperm and Other Growth Stimulants upon the Development In vitro of Embryos of Cocos nucifera

Cited by 41 publications

References 0 publications

In vitro regeneration of coconut (Cocos nucifera L) through indirect somatic embryogenesis in Kenya

In vitro regeneration of coconut (Cocos nucifera L) through indirect somatic embryogenesis in Kenya

Nutrition, Growth and Morphogenesis of Plant Embryos

A Report on the Culture of Embryos of Dwarf Coconut, <i>Cocos nucifera</i> L var <i>nana In vitro</i>

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