Effect of Co-culturing both placenta-derived mesenchymal stem cells and their condition medium in the cancer cell (HepG2) migration, damage through apoptosis and cell cycle arrest

Opo, FA Dain Md; Moulay, Mohammed; Alrefaei, Ghadeer I.; Alsubhi, Nouf H.; Alkarim, Saleh; Rahman, Mohammed M.

doi:10.1016/j.sjbs.2022.103519

Cited by 3 publications

(1 citation statement)

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“…In MOF-5, the apoptotic cells were 56.90% (31.46% early and 25.44% late) (*P < 0.05) (Fig. 7b) [53], and the necrosis cell enumerated was 0.6%. Evaluations of apoptotic populations utilizing ow cytometry in the MCF-7 cells provided that NG at IC 50 concentration (188 µg/mL) prompted 60.59% apoptosis cells (11.15% late and 49.54% early) (*P < 0.05) (Fig.…”

Section: In Vitro Cytotoxicitymentioning

confidence: 95%

Investigation and optimization of naringin-loaded in MOF-5 encapsulated by liponiosomes as smart drug delivery, cytotoxicity, and apoptotic on breast cancer cells

Elbeltagi,

Alharbi,

Aodah

et al. 2024

Preprint

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Tumors are considered dangerous due to their significant global mortality rate, with breast cancer (BC) being a major contributor to this statistic, impacting almost all tumor patients worldwide. Conventional methods for BC treatment, such as special naringin (NG) chemotherapy based on nanotechnology components, have been insufficient, and a new approach has been used to improve drug delivery (DD) to cancer. Herein, developed biocompatible nanoparticles (NPs) comprising NG-loaded zinc metal-organic framework-5 (NG-MOF-5) encapsulated with liponiosomes (LNs) to create NG-MOF-5@LNs. Various biophysical characteristics of free NG, MOF-5, and NG-MOF-5@LNs NPs were studied, including XRD, FTIR, TEM, SEM, zeta potential (ZP), hydrodynamic size, PDI, loading efficiency (LE), encapsulation efficiency (EE), drug release (DR), and drug-release kinetics. TEM images showed that NG-MOF-5@LNs NPs consisted of monodispersed spherical-like particles with average diameters of 122.5 nm (*P < 0.05). The optimized NG-MOF-5@LNs exhibited a particle size (PDI: 139.8 nm) and a ZP (+ 21 mV). The EE and LE for NG-MOF-5@LNs were 89.15% and 14.05%, respectively. Four kinetic models were examined to understand the NG release mechanism at pH 6.9, with the Higuchi model indicating excellent fit (R2 = 0.9988). The MTT cell viability assay demonstrated anti-cancer activity against MCF-7 BC cells, with inhibitory concentrations (IC50) of 231.9, 188.2, and 21.6 µg/mL (*P < 0.05) for MOF-5, NG, and NG-MOF-5@LNs, respectively, indicating an apoptosis effect on MCF-7 cells. NG-MOF-5@LNs is expected to emerge as a novel long-term-release anticancer agent. NG-MOF-5@LNs may represent a superior option for BC therapy compared to conventional NG formulations.

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Section: In Vitro Cytotoxicitymentioning

confidence: 95%