Effect of Co-administration of Aconiti Lateralis Preparata Radix and Cinnamomi Cortex on Osteoclast Differentiation

Abstract

Objectives : Aconiti Lateralis Preparata Radix (Aconitum Carmichaeli, AC) and Cinnamomi Cortex (Cinnamomi Cortex, CC) have been treated to elderly for kidney yang enhancement in Korean traditional medicine. In this study, the effects of water extract of AC and CC on RANKL (Receptor Activator for Nuclear Factor κ B Ligand)-induced osteoclast differentiation were evaluated in culture system. Methods : MTT assay was used to evaluate the potential cytotoxicity of AC and CC extracts in bone macrophage marrows (BMMs) stimulated with M-CSF. TRAP (tartrate-resistant acid phosphatase) staining and TRAP activity were performed to know the inhibitory effect on osteoclast differentiation. The protein expression levels of nuclear factors such as activated T cell(NFAT)c1, c-Fos, MAPKs and β -actin in cell lysates treated with AC and CC extracts were analysed by western blotting. Results : AC, CC extracts and their co-administration inhibited significantly RANKL-induced osteoclast differentiation in BMMs in a dose dependent manner without toxicity. Each AC and CC extracts inhibited the phosphorylation of p38. Also, AC and CC extracts, respectively, inhibited the protein expression of c-Fos and NFATc1 more than Co-administration of AC and CC even if all treatments did. It was observed that RANKL-induced degradation of I-κ B is significantly suppressed by all treatments. Conclusions : Taken together, It was concluded that AC and CC have beneficial effect on osteoporosis by inhibition of osteoclast differentiation. Thus, Atractylodis AC and CC could be a treatment option for osteoporosis.

Antioxidant and Anti-inflammatory Responses of Pycnogenol, Hibiscus Flower Extract, and Niacin in Skin Epithelial Cells

“…In particular, a substance having an anti-inflammatory action [3] has a soothing effect on the skin. This has also been shown in studies of compounds with anti-inflammatory effects in acne and atopic dermatitis [26].…”

Inhibitory Effects of Yongbu-tang on Osteoclast Differentiation and Bone Resorption※

“…부자에 관한 연구를 살펴보면 부자와 육계를 병용 투여 시 파골세포 분화가 억제된다는 보고가 있고 13) , 그 외에도 진통 및 소염작용, 관절염, 척수손상, 비만 등에 관한 연구 가 보고되어 있다 [10][11][12]14) . (Fig.…”

“…녹용(鹿茸, Cervi Parvum Cornu)은 대표적인 補腎, 强 筋骨, 補陽藥類로 4) , 여러 연구에서 골다공증, 류마티스 관 절염, 성장촉진, 면역력 증강 등에 효과가 있는 것으로 보 고되어 있다 [5][6][7][8][9] . 부자(附子, Aconiti Lateralis Radix Preparata)는 溫 熱藥類에 속하며 4) , 진통 및 소염작용, 관절염, 척수손상, 파골세포 분화, 비만 등에 관한 연구가 보고되어 있다 [10][11][12][13][14] . 녹용과 부자로 구성된 처방인 용부탕(茸附湯)은 ≪東醫寶 鑑 虛勞門≫의 陽虛用藥에 나온 처방으로 氣精血虛耗, 潮 熱, 盜汗을 치료한다고 하였다 15) .…”

“…현재까지 골다공증에 대한 연구로 鹿茸 附子 紫河車 續斷 杜冲 人蔘 紅花子 骨碎補 牛膝 등의 단일 한약 재와 獨活寄生湯, 加味左歸飮, 歸腎丸, 三氣飮加味方 등의 한 약 처방에 대한 연구가 보고 되어 있다 5,6,13,16) . 실험 방법 적인 면에서 난소 적출하여 골다공증을 유발한 mouse나 rat을 이용한 실험 연구가 많았으나 5,13) , 파골세포 분화 억 제와 골 흡수 억제에 대한 실험 연구가 점점 증가하고 있다 6,13) . 그러나…”