Effect of Cleome arabica leaf extract, rutin and quercetin on soybean lipoxygenase activity and on generation of inflammatory eicosanoids by human neutrophils

Bouriche, Hamama; Miles, Elizabeth A.; Selloum, Laid; Calder, Philip C.

doi:10.1016/j.plefa.2004.10.018

Cited by 30 publications

(24 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The flavonoid glycosides rutin and narcissin were both fairly good inhibitors of 15-LO. This is in accordance with the previous results (Bouriche et al, 2007) for rutin. Narcissin, however, was reported to be inactive (Robak et al, 1988).…”

Section: Resultssupporting

confidence: 94%

α-Glucosidase inhibition, 15-lipoxygenase inhibition, and brine shrimp toxicity of extracts and isolated compounds fromTerminalia macropteraleaves

Pham

Malterud

Paulsen

et al. 2014

Pharmaceutical Biology

View full text Add to dashboard Cite

Context: Terminalia macroptera Guill. & Perr. (Combretaceae), a tree that grows in West Africa, has been used in traditional medicine against a variety of diseases such as hepatitis, gonorrhea, skin diseases, and diabetes. Objective: To investigate enzyme inhibitory activity against a-glucosidase and 15-lipoxygenase (15-LO) and toxicity against brine shrimp of extracts and compounds from T. macroptera leaves. Materials and methods: Methanol extract, ethyl acetate, and butanol extracts obtained from the methanol extract, six isolated polyphenols (chebulagic acid, chebulic acid trimethyl ester, corilagin, methyl gallate, narcissin, and rutin), and shikimic acid were evaluated for enzyme inhibition and toxicity. Results: In enzyme inhibition assays, all extracts showed high or very high activity. Chebulagic acid showed an IC 50 value of 0.05 mM towards a-glucosidase and 24.9 ± 0.4 mM towards 15-LO, in contrast to positive controls (acarbose: IC 50 201 ± 28 mM towards a-glucosidase, quercetin: 93 ± 3 mM towards 15-LO). Corilagin and narcissin were good 15-LO and a-glucosidase inhibitors, as well, while shikimic acid, methyl gallate, and chebulic acid trimethyl ester were less active or inactive. Rutin was a good a-glucosidase inhibitor (IC 50 ca. 3 mM), but less active towards 15-LO. None of the extracts or the isolated compounds seemed to be very toxic in the brine shrimp assay compared with the positive control podophyllotoxin. Conclusion: Inhibition of a-glucosidase in the gastrointestinal tract may be a rationale for the medicinal use of T. macroptera leaves against diabetes in traditional medicine in Mali. The plant extracts and its constituents show strong inhibition of the peroxidative enzyme 15-LO.

show abstract

Section: Resultssupporting

confidence: 94%

α-Glucosidase inhibition, 15-lipoxygenase inhibition, and brine shrimp toxicity of extracts and isolated compounds fromTerminalia macropteraleaves

Pham

Malterud

Paulsen

et al. 2014

Pharmaceutical Biology

View full text Add to dashboard Cite

show abstract

“…In the case of UVB-irradiated keratinocytes, ascorbic acid downregulated IL-8 mRNA expression [36]. It has also been reported that rutin treatment of neutrophils inhibits the activity of enzymes responsible for the metabolism of fatty acids, cyclooxygenase (COX) and lipoxygenase (LOX), thereby reducing pro-inflammatory factors amount [8,24], what in this study was observed as a significant decrease in 8-isoPGF2α level. Moreover, rutin suppresses NFκB activation and down-regulates gene expression of most proinflammatory cytokines/enzymes, except for a significant up-regulation of IL-8 observed under stimulation with TGFα [61].…”

Section: Inflammationsupporting

confidence: 60%

“…Despite its antioxidant properties, rutin influences cellular metabolism. Rutin is a flavonoid found in plant extracts, and inhibits the activities of cyclooxygenases and lipoxygenases, thereby reducing pro-inflammatory processes [8]. Moreover, rutin exerts cytoprotective effects on cells exposed to various types of radiation by substantially increasing their viability [79].…”

Section: Introductionmentioning

confidence: 99%

Rutin and ascorbic acid cooperation in antioxidant and antiapoptotic effect on human skin keratinocytes and fibroblasts exposed to UVA and UVB radiation

et al. 2019

View full text Add to dashboard Cite

The combination of ascorbic acid and rutin is frequently used in oral preparations. However, despite numerous protective effects of each component individually, their combined effect on ultraviolet (UV)-irradiated skin cells has never been evaluated. The aim of this study was to evaluate the combined effect of ascorbic acid and rutin on human keratinocytes and fibroblasts exposed to UVA and UVB radiation. Skin keratinocytes and fibroblasts exposed to UVA and UVB radiation were treated with ascorbic acid or/and rutin. The total antioxidant properties of both components, as well as their effect on cellular pro-and antioxidant status, lipid and protein oxidation, transmembrane transport, and pro-inflammatory and pro/ antiapoptotic protein expression were measured. The combination of ascorbic acid and rutin had higher antioxidant properties compared to the activity of the single compound alone, and showed a stronger effect against UV-induced reactive oxygen species generation. The ascorbic acid and rutin combination also showed increased antioxidant enzyme activity (catalase, superoxide dismutase, thioredoxin reductase), which was impaired following UV irradiation. Moreover, ascorbic acid additional stimulated UV-induced bilitranslocase activity responsible for rutin transport, and therefore favored rutin effect on Nrf2 pathway, simultaneously differentiating the reaction of keratinocytes and fibroblasts. In keratinocytes, Nrf2 is strongly activated, while in fibroblasts decreased Nrf2 activity was observed. Used mixture, also significantly silenced UV-induced expression of pro-inflammatory factor NFκB and pro-apoptotic proteins such as caspases 3, 8, and 9. These results showed that ascorbic acid and rutin are complementary in their antioxidant actions, transport and signaling functions. Their combined antioxidant, antiinflammatory and antiapoptotic actions suggest rutin and ascorbic acid are a potentially cytoprotective team against UV-induced skin damage.

show abstract

“…Rutin and its hydrolysis product quercetin have demonstrated to inhibit soybean LOX activity in a dose-dependent manner, with IC 50 values of 14 and 11 μM [28]. Isorhamnetin, the hydrolysis product of IRH, has been shown to prevent acute inflammation through blocking NF-κB activation [29] and shows antioxidant activity [30].…”

Section: Resultsmentioning

confidence: 99%

Anti-Inflammatory Activity of Copao (Eulychnia Acida Phil., Cactaceae) Fruits

Jiménez‐Aspee

Arena

Quispe

et al. 2015

Plant Foods Hum Nutr

View full text Add to dashboard Cite

Copao (Eulychnia acida Phil., Cactaceae) is an endemic species occurring in northern Chile. The edible fruits of this plant are valued for its acidic and refreshing taste. Phenolic-enriched extracts from copao fruit pulp and epicarp, collected in the Elqui and Limari river valleys, were assessed by its in vitro ability to inhibit the pro-inflammatory enzymes lipoxygenase (LOX) and cyclooxygenases (COX-1 and COX-2). At 100 μg/mL, pulp extracts showed better effect towards LOX than epicarp extract, while COX-2 inhibition was observed for both epicarp and pulp samples. In general, the extracts were inactive towards COX-1. A positive correlation was observed between the anti-inflammatory activity and the main phenolic compounds found in this fruit. Copao fruits from the Limari valley, a main place of collection and commercialization, showed major activity, adding evidence on the possible health-beneficial effects of this native Chilean fruit.

show abstract

Effect of Cleome arabica leaf extract, rutin and quercetin on soybean lipoxygenase activity and on generation of inflammatory eicosanoids by human neutrophils

Cited by 30 publications

References 30 publications

α-Glucosidase inhibition, 15-lipoxygenase inhibition, and brine shrimp toxicity of extracts and isolated compounds fromTerminalia macropteraleaves

α-Glucosidase inhibition, 15-lipoxygenase inhibition, and brine shrimp toxicity of extracts and isolated compounds fromTerminalia macropteraleaves

Rutin and ascorbic acid cooperation in antioxidant and antiapoptotic effect on human skin keratinocytes and fibroblasts exposed to UVA and UVB radiation

Anti-Inflammatory Activity of Copao (Eulychnia Acida Phil., Cactaceae) Fruits

Contact Info

Product

Resources

About