Effect of Cleome arabica Leaf Extract on Rat Paw Edema and Human Neutrophil Migration

Bouriche, Hamama; Selloum, Laid; Tigrine, Chafia; Boudoukha, Chahra

doi:10.1076/phbi.41.1.10.14698

Cited by 23 publications

(14 citation statements)

References 18 publications

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“…However, new research is needed to clarify this point. On the other hand, the delayed phase of the carrageenan test (3-6 h) has been linked to release of prostaglandins, arachidonate metabolites, neutrophil migration, release of oxygen free radicals, proteolytic enzymes, as well as other neutrophil-derived mediators (Boughton-Smith et al, 1993;Bouriche et al, 2003). The ALE contained flavonoids and tannins.…”

Section: Discussionmentioning

confidence: 98%

Anti-inflammatory Activity of the Aqueous Leaf Extract ofIxora coccinea.

Ratnasooriya

Deraniyagala

Galhena

et al. 2005

Pharmaceutical Biology

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The aim of this study was to investigate the antiinflammatory potential of an aqueous leaf extract (ALE) of Ixora coccinea (Rubiaceae) in rats after oral administration (500, 1000 and 1500 mg=kg). This was done using the carrageenan-induced paw edema (acute inflammatory model) and cotton pellet granuloma tests (chronic inflammatory model). In the former test, ALE significantly impaired both early and late phases of the inflammatory response and also the edema maintained between the two phases. In the latter test, it significantly suppressed granuloma formation (only highest dose tested). Collectively, these data show promising anti-inflammatory activity against both acute and chronic inflammation. ALE showed strong antihistamine and antioxidant activities that can account for its anti-inflammatory potential. In addition, inhibition of prostaglandins and bradykinins may play a role.

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Section: Discussionmentioning

confidence: 98%

Anti-inflammatory Activity of the Aqueous Leaf Extract ofIxora coccinea.

Ratnasooriya

Deraniyagala

Galhena

et al. 2005

Pharmaceutical Biology

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“…Less information is available on botanical extracts that contain a mixture of flavonoids. Cleome arabica is well known in traditional medicine: its botanical extract with high antioxidant capacities (Djeridane et al, 2010;Selloum et al, 2004) could reduce inflammatory conditions both in vivo and in vitro (Bouriche et al, 2003. These effects have been related to the flavonoid content of CALE.…”

Section: Discussionmentioning

confidence: 99%

“…2007-1) has been deposited in the Laboratory of Botany, Department of Biology, University of Setif, Algeria. Plant extract was prepared as previously described (Bouriche et al, 2003). In brief, the air-dried leaves (100 g) were powdered and extracted three-times with methanol/water (7/3, v/v) for 24 h. The collected filtrates were concentrated under reduced pressure and the boiled water was added to residue and kept for 24 h at 4 C. Then, the addition of ethyl acetate to aqueous layer yielded to the formation of a yellow precipitate.…”

Section: Chemicals and Reagentsmentioning

confidence: 99%

“…It has been reported that the polyphenolic extract of C. arabica leaves (CALE) could reduce inflammatory conditions either in vivo by reducing rat paw edema, in vitro by modulating the activity of lipoxygenase and calcium-stimulated generation of the leukotriene B4 and prostaglandin E2 in human neutrophils or also by inhibiting their chemotaxis (Bouriche et al, 2003(Bouriche et al, , 2004. These CALE actions seem to be related to the high content of polyphenolic compounds (Bouriche et al, 2003;Djeridane et al, 2010;Ismail et al, 2005). Indeed, C. arabica leaf extract contains flavonoids such as glycosylated quercetin, kaempferol and isorhamnetin (Ismail et al, 2005) that also increase the antioxidant capacities of this extract (Djeridane et al, 2010;Selloum et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Cleome arabicaleaf extract has anticancer properties in human cancer cells

Tigrine

Bulzomi

Leone

et al. 2013

Pharmaceutical Biology

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Context: Cleome arabica L. (Capparidaceae) is a desert plant widely distributed in the North part of Africa whose leaves are used in traditional medicine as a sedative for abdominal and rheumatic pains. Objectives: The anticancer activity of methanol Cleome arabica leaf extracts (CALE) is investigated in different human cancer cell lines. Materials and methods: Five different human cancer cell lines, representative of the most common cancers in Western countries (i.e., breast adenocarcinoma, colon carcinoma, neuroblastoma, hepatoma, cervix carcinoma) were treated with different concentrations of CALE (i.e., 1, 5, 10, 25, 50, 100 and 200 mg/ml). Cell viability and cell cycle were measured by using a hemocytometer chamber and a cytofluorimeter, respectively. Epidermal growth factor (EGF) was used as a positive control. Western blots were performed to evaluate the CALE effects on pathways involved in cell growth regulation and on apoptotic cascade activation. Results and conclusion: Our results confirm that CALE has a high content of polyphenolic compounds (i.e., 32.21 AE 3.44%), mainly as flavonoids (24.56 AE 4.67%). In all tested cell lines CALE treatment reduces cell number in a dose-dependent manner (ED 50 ¼ 175 AE 30 mg/ml). CALE (100 and 200 mg/ml) increases by three-fold the activation of the apoptotic cascade involving caspase-3 activation and the cleavage of its substrate poly(ADP-ribose) polymerase (PARP). Intriguingly, CALE treatment (200 mg/ml) also blocks EGF-induced cell growth by preventing the growth factor-triggered AKT and ERK phosphorylation. As a whole, these data strongly suggest that CALE possesses anticancer effects in all tested cancer cell lines.

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“…The development of carrageenan-induced oedema is biphasic (Vinegar et al, 1987); the first phase occurs within one hour of carrageenan administration and this is attributed to the release of cytoplasmic enzymes, histamine and serotonin, from mast cells. The second phase (over one hour) is mediated by an increased release of prostaglandins, arachidonate metabolites, neutrophil migration, release of oxygen free radicals, proteolytic enzymes, as well as other neutrophil-derived mediators (Bouriche et al, 2003). The oedema maintained between the first and second phase is due to kinin-like substances, especially bradykinin (Vinegar et al, 1987).…”

Section: Abiodun Falodun; Ighodaro Igbe; Osayemwenre Erharuyi; Onyinymentioning

confidence: 99%

Chemical Characterization, Anti inflammatory and Analgesic Properties of <i>Jatropha Multifida</i> Root Bark

Falodun¹,

Igbe²,

Erharuyi³

et al. 2013

Journal of Applied Sciences and Environmental Management

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Jatropha multifida is used in African folk medicine for the treatment of infection, pain, fever, various inflammatory conditions, tumor and tumor related diseases. The present study investigated the phytochemical constituents of the powdered root bark using established standard procedures. The methanol root bark extract at doses of 100 mg/kg, 200 mg/kg and 400 mg/kg was subjected to evaluation of anti-inflammatory and analgesic activities in experimental animal models. The anti-inflammatory activity was estimated by measuring the mean thickness in carrageenan-induced hind paw oedema in Wistar albino rats. The analgesic activity was assessed by acetic acid-induced writhing in Swiss albino mice. Indomethacin (10 mg/kg) and acetyl salicylic acid (100 mg/kg) were used as standards in the anti-inflammatory and analgesic assay respectively. Phytochemical investigations reveal the presence of alkaloids, tannins, glycosides, saponins and flavonoids. The extract at a dose of 400 mg/kg significantly (P < 0.01) reduced paw thickness in rat compared to control. There was a dose dependent decrease in writhings which was significant (P < 0.05) at 400 mg/kg compared to control. The results therefore suggest that J. Multifida root bark has anti-inflammatory and analgesic activities which further substantiate its use in folk medicine.@ JASEM http://dx.doi.org/10.4314/jasem.v17i3.3

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Effect of Cleome arabica Leaf Extract on Rat Paw Edema and Human Neutrophil Migration

Cited by 23 publications

References 18 publications

Anti-inflammatory Activity of the Aqueous Leaf Extract ofIxora coccinea.

Anti-inflammatory Activity of the Aqueous Leaf Extract ofIxora coccinea.

Cleome arabicaleaf extract has anticancer properties in human cancer cells

Chemical Characterization, Anti inflammatory and Analgesic Properties of <i>Jatropha Multifida</i> Root Bark

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