Effect of Ciprofloxacin, Levofloxacin, and Ofloxacin on Pseudomonas aeruginosa : A case control study with time kill curve analysis

Sihotang, Tiar Sondang Uli; Widodo, Agung Dwi Wahyu; Endraswari, Pepy Dwi

doi:10.1016/j.amsu.2022.104674

Cited by 6 publications

(5 citation statements)

References 29 publications

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“…Inspired by the study conducted by Lv et al (2023) , who employed not only common bacteria, such as E. coli , S. aureus , methicillin-resistant S. aureus , and V. parahaemolyticus , but also marine bacteria ( V. vulnificus and P. aeruginosa ) to evaluate the antibacterial effect of hydrogel on seawater-immersed wounds, a mixed microbial suspension was prepared to simulate the dominant pathogenic flora of seawater immersion wound in our study, therefore, ensuring that the irrigation solution was suitable for further practical application. On the other hand, although the phenomenon of colony number reduction and then growth often was observed in the time–killing test of P. aeruginosa when evaluating the antibacterial effect of ciprofloxacin, levofloxacin, and ofloxacin ( Sihotang et al, 2022 ), this did not occur in our study, and a complete eradication was reached after 2 h treatment, proving its viability as a wound irrigation solution to reduce the microbial load.…”

Section: Resultscontrasting

confidence: 49%

Antimicrobial efficacy of composite irrigation solution against dominant pathogens in seawater immersion wound and in vivo wound healing assessment

Wang

Tan

et al. 2023

Front. Microbiol.

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Seawater immersion wound is inevitably accompanied by bacterial infection. Effective irrigation is critical for bacterial infection prevention and wound healing. In this study, the antimicrobial efficacy of a designed composite irrigation solution against several dominant pathogens in seawater immersion wounds was evaluated, and in vivo wound healing assessment was conducted in a rat model. According to the time–kill result, the composite irrigation solution exhibits excellent and rapid bactericidal effect against Vibrio alginolyticus and Vibrio parahaemolyticus within 30 s of treatment while eliminating Candida albicans, Pseudomonas aeruginosa, Escherichia coli, and the mixed microbes after 1 h, 2 h, 6 h, and 12 h of treatment, respectively. Significant bacterial count reduction of Staphylococcus aureus was observed after 5 h treatment. In addition to its skin non-irritating attribute, the in vivo wound healing results further demonstrated that the irrigation solution showed high repair efficiency in the skin defect model inoculated with the mixed microbes. The wound healing rate was significantly higher than that of the control and normal saline groups. It could also effectively reduce the number of viable bacteria on the wound surface. The histological staining indicated that the irrigation solution could reduce inflammatory cells and promote collagen fibers and angiogenesis, thereby promoting wound healing. We believed that the designed composite irrigation solution has great potential for application in the treatment of seawater immersion wounds.

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Section: Resultscontrasting

confidence: 49%

Antimicrobial efficacy of composite irrigation solution against dominant pathogens in seawater immersion wound and in vivo wound healing assessment

Wang

Tan

et al. 2023

Front. Microbiol.

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“…The time-kill study has been commonly employed to assess the concentration level of an antimicrobial agent with bactericidal properties. Such bactericidal activity can be exploited in the treatment of bacterial infections in concentration and time-dependent manner [ 47 ]. It is an effective technique to study the dynamic interaction between the antimicrobial agent under study and the microbial strain [ 48 ].…”

Section: Resultsmentioning

confidence: 99%

Aluminum oxide, cobalt aluminum oxide, and aluminum-doped zinc oxide nanoparticles as an effective antimicrobial agent against pathogens

Omeiri,

El Hadidi,

Awad

et al. 2024

Heliyon

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“…levofloxacin is an antibiotic from the fluoroquinolone class and belongs to the third generation, while ofloxacin is an antibiotic from the same class and belongs to the second generation. Therefore, levofloxacin was chosen because it is more effective and potent against MRSA and other bacteria than ofloxacin. − The inhibition zone (IZ) was measured in millimeters, and the results are summarized in Table .…”

Section: Resultsmentioning

confidence: 99%

New Pyrazole-Clubbed Pyrimidine or Pyrazoline Hybrids as Anti-Methicillin-Resistant Staphylococcus aureus Agents: Design, Synthesis, In Vitro and In Vivo Evaluation, and Molecular Modeling Simulation

Mansour,

El-Sherbeny,

Al-Omary

et al. 2023

ACS Omega

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Two hybrid series of pyrazole-clubbed pyrimidines 5a−c and pyrazole-clubbed pyrazoline compounds 6a,b and 7 were designed as attractive scaffolds to be investigated in vitro and in vivo for antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa. From the results of the in vitro antibacterial screening, compound 5c showed excellent activity (minimal inhibitory concentration, MIC = 521 μM) when compared with that of the reference antibiotic levofloxacin (MIC = 346 μM). The inhibition of the target dihydrofolate reductase (DHFR) enzyme by compounds 4 and 5a−c (IC 50 = 5.00 ± 0.23, 4.20 ± 0.20, 4.10 ± 0.19, and 4.00 ± 0.18 μM, respectively) was found to be better than the reference drug trimethoprim (IC 50 = 5.54 ± 0.28 μM). Molecular modeling simulation results have justified the order of activity of all the newly synthesized compounds as DHFR enzyme inhibitors, and compound 5c exhibited the best binding profile (−13.6169386 kcal/mol). Hence, the most potent inhibitor of the DHFR enzyme, 5c, was chosen to be evaluated in vivo for its activity in treating MRSA-induced keratitis in rats and that, in turn, significantly (P < 0.0001) reduced infection in rats when compared to MRSA-treated group results.

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Effect of Ciprofloxacin, Levofloxacin, and Ofloxacin on Pseudomonas aeruginosa : A case control study with time kill curve analysis

Cited by 6 publications

References 29 publications

Antimicrobial efficacy of composite irrigation solution against dominant pathogens in seawater immersion wound and in vivo wound healing assessment

Antimicrobial efficacy of composite irrigation solution against dominant pathogens in seawater immersion wound and in vivo wound healing assessment

Aluminum oxide, cobalt aluminum oxide, and aluminum-doped zinc oxide nanoparticles as an effective antimicrobial agent against pathogens

New Pyrazole-Clubbed Pyrimidine or Pyrazoline Hybrids as Anti-Methicillin-Resistant Staphylococcus aureus Agents: Design, Synthesis, In Vitro and In Vivo Evaluation, and Molecular Modeling Simulation

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