Effect of chronic stress on expression and secretion of seminal vesicle proteins in adult rats

Iamsaard, Sitthichai; Tongpan, Saranya; Yannasithinon, Supataechasit; Arun, Supatcharee; Wu, Alexander T.H.; Sukhorum, Wannisa

doi:10.1111/and.13800

Cited by 11 publications

(14 citation statements)

References 51 publications

(50 reference statements)

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“…It is novel that CS could change the levels of ions in epididymal fluid. Additionally, reduction of fructosamine levels in the epididymal fluid observed in our study was totally similar to infertile animal models showing decreased fructosamine in both blood serum and seminal fluid (van Eijk et al., 2007; Iamsaard et al., 2020; Tongpan et al., 2019). Particularly, this study is agreed with the previous investigations revealed that CS significantly decreased the fructosamine level in the head epididymal fluid (van Eijk et al., 2007; Tongpan et al., 2019).…”

Section: Discussionsupporting

confidence: 88%

“…Present results also found the decrease of epididymal weight in CS rat, assumed that epididymal AR expression was decreased since its testosterone level significantly decreased (Table 1). It has been revealed that HSP‐70 is expressed in the testis, seminal vesicle, epididymis under both normal and stressed conditions (Iamsaard et al., 2020; Tongpan et al., 2019; Wang et al., 2016). In epididymis, HSP‐70 is present in the basal cells protecting sperm apoptosis and responsible for autoimmune defence (Cao et al., 2009; Purandhar et al., 2014; Wang et al., 2016).…”

Section: Discussionmentioning

confidence: 99%

“…A previous study has localised the TyrPho in the cytoplasm of principle cells, nuclei of apical and basal cells, and fluid of rat caudal epididymis (Sawatpanich et al., 2018). Although the actual functions of TyrPho proteins in epididymal fluid need to be further elucidated, previous studies have shown the alteration of TyrPho protein expressions in several subfertile models including stress (Arun et al., 2016; Iamsaard et al., 2020; Tangsrisakda & Iamsaard, 2020; Sampannang et al., 2019; Sukhorum & Iamsaard, 2017; Tongpan et al., 2019; Yannasithinon & Iamsaard, 2019; Yannasithinon & Iamsaard, 2020). Under CS investigated in this study, the TyrPho protein expressions of epididymal fluids and head epididymal tissue were mostly shown for the first time to be decreased but increased in the tail epididymal tissue.…”

Section: Discussionmentioning

confidence: 99%

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Chronic stress affects tyrosine phosphorylated protein expression and secretion of male rat epididymis

et al. 2021

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Chronic stress (CS) is shown to decrease the semen quality with changed expression of tyrosine phosphorylated (TyrPho) proteins in testicular and seminal tissues. However, the alterations of such proteins and fluid contents in the epididymis, producing sperm maturation factors, have never been reported. Sixteen adult rats were randomly divided into 2 groups (n = 8). The control animals were not subjected to stressors whereas CS rats were immobilised within restraint cage (4 hr/day) before cold forced‐water swimming (15 min/day) for 60 days. Corticosterone, testosterone, blood glucose level (BGL), malondialdehyde (MDA) and biochemical components in epididymal fluid were assayed. Expressions of heat shock protein 70 (HSP‐70), androgen receptor (AR) and TyrPho protein were investigated in epididymal tissue and fluid. Significantly, CS increased the corticosterone and BGL but decreased testosterone and epididymal substance levels. MDA level in tail epididymal fluid and HSP‐70 expression in both regions of epididymal tissues and fluids, except in head epididymal fluid of CS were increased. Epididymal tissues showed the decrease of AR expression. Presence and changes of many TyrPho proteins were observed in CS. In conclusion, CS could affect functional proteins particularly TyrPho in epididymis, resulted in low semen quality.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Chronic stress affects tyrosine phosphorylated protein expression and secretion of male rat epididymis

et al. 2021

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“…Notably, the composition of seminal vesicle secretions is known to exhibit significant plasticity in response to changing paternal environments ( 63 , 64 , 65 ) or even cues produced by a female partner ( 66 ); functions that are postulated to maximize fertilization success and transmit evolutionary adaptations to offspring ( 63 , 66 , 67 ). These above studies raise the possibility that in addition to sperm, the seminal vesicles are sensitive to environmental signals and stressors ( 3 , 15 , 17 ).…”

Section: Discussionmentioning

confidence: 99%

Proteomic Dissection of the Impact of Environmental Exposures on Mouse Seminal Vesicle Function

Skerrett‐Byrne

Trigg

Bromfield

et al. 2021

Molecular & Cellular Proteomics

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Seminal vesicles are an integral part of the male reproductive accessory gland system. They produce a complex array of secretions containing bioactive constituents that support gamete function and promote reproductive success, with emerging evidence suggesting these secretions are influenced by our environment. Despite their significance, the biology of seminal vesicles remains poorly defined. Here, we complete the first proteomic assessment of mouse seminal vesicles and assess the impact of the reproductive toxicant acrylamide. Mice were administered acrylamide (25 mg/kg bw/day) or control daily for five consecutive days prior to collecting seminal vesicle tissue. A total of 5013 proteins were identified in the seminal vesicle proteome with bioinformatic analyses identifying cell proliferation, protein synthesis, cellular death, and survival pathways as prominent biological processes. Secreted proteins were among the most abundant, and several proteins are linked with seminal vesicle phenotypes. Analysis of the effect of acrylamide on the seminal vesicle proteome revealed 311 differentially regulated (FC ± 1.5, p ≤ 0.05, 205 up-regulated, 106 downregulated) proteins, orthogonally validated via immunoblotting and immunohistochemistry. Pathways that initiate protein synthesis to promote cellular survival were prominent among the dysregulated pathways, and rapamycin-insensitive companion of mTOR (RICTOR, p = 6.69E-07) was a top-ranked upstream driver. Oxidative stress was implicated as contributing to protein changes, with acrylamide causing an increase in 8-OHdG in seminal vesicle epithelial cells (fivefold increase, p = 0.016) and the surrounding smooth muscle layer (twofold increase, p = 0.043). Additionally, acrylamide treatment caused a reduction in seminal vesicle secretion weight (36% reduction, p = 0.009) and total protein content (25% reduction, p = 0.017). Together these findings support the interpretation that toxicant exposure influences male accessory gland physiology and highlights the need to consider the response of all male reproductive tract tissues when interpreting the impact of environmental stressors on male reproductive function.

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“…Notably, seminal vesicle secretions exhibit high levels of plasticity in response to changing paternal environments [11][12][13]. Several studies report seminal vesicle responsiveness to paternal physiological disturbances and environmental insults such as metabolic disorder [14], nutritional deficiency [15], endocrine disrupting compound exposure [16], and heat or psychosocial stress [13,17].…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic analysis of the seminal vesicle response to the reproductive toxicant acrylamide

et al. 2021

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Background The seminal vesicles synthesise bioactive factors that support gamete function, modulate the female reproductive tract to promote implantation, and influence developmental programming of offspring phenotype. Despite the significance of the seminal vesicles in reproduction, their biology remains poorly defined. Here, to advance understanding of seminal vesicle biology, we analyse the mouse seminal vesicle transcriptome under normal physiological conditions and in response to acute exposure to the reproductive toxicant acrylamide. Mice were administered acrylamide (25 mg/kg bw/day) or vehicle control daily for five consecutive days prior to collecting seminal vesicle tissue 72 h following the final injection. Results A total of 15,304 genes were identified in the seminal vesicles with those encoding secreted proteins amongst the most abundant. In addition to reproductive hormone pathways, functional annotation of the seminal vesicle transcriptome identified cell proliferation, protein synthesis, and cellular death and survival pathways as prominent biological processes. Administration of acrylamide elicited 70 differentially regulated (fold-change ≥1.5 or ≤ 0.67) genes, several of which were orthogonally validated using quantitative PCR. Pathways that initiate gene and protein synthesis to promote cellular survival were prominent amongst the dysregulated pathways. Inflammation was also a key transcriptomic response to acrylamide, with the cytokine, Colony stimulating factor 2 (Csf2) identified as a top-ranked upstream driver and inflammatory mediator associated with recovery of homeostasis. Early growth response (Egr1), C-C motif chemokine ligand 8 (Ccl8), and Collagen, type V, alpha 1 (Col5a1) were also identified amongst the dysregulated genes. Additionally, acrylamide treatment led to subtle changes in the expression of genes that encode proteins secreted by the seminal vesicle, including the complement regulator, Complement factor b (Cfb). Conclusions These data add to emerging evidence demonstrating that the seminal vesicles, like other male reproductive tract tissues, are sensitive to environmental insults, and respond in a manner with potential to exert impact on fetal development and later offspring health.

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Effect of chronic stress on expression and secretion of seminal vesicle proteins in adult rats

Cited by 11 publications

References 51 publications

Chronic stress affects tyrosine phosphorylated protein expression and secretion of male rat epididymis

Chronic stress affects tyrosine phosphorylated protein expression and secretion of male rat epididymis

Proteomic Dissection of the Impact of Environmental Exposures on Mouse Seminal Vesicle Function

Transcriptomic analysis of the seminal vesicle response to the reproductive toxicant acrylamide

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