Effect of chronic mild stress and imipramine on the proteome of the rat dentate gyrus

Kędracka–Krok, Sylwia; Fic, Ewelina; Jankowska, Urszula; Jaciuk, Marcin; Gruca, Piotr; Papp, Mariusz; Kuśmider, Maciej; Solich, Joanna; Dębski, Janusz; Dadlez, Michał; Dziedzicka-Wasylewska, Marta

doi:10.1111/j.1471-4159.2010.06652.x

Cited by 29 publications

(18 citation statements)

References 65 publications

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“…In support of a theory on CMS-induced alterations of vesicular-related proteins, a recent study on punches of dentate gyrus tissue from CMS-exposed rats also showed alterations in vesicular-related proteins (67). Moreover, an earlier study at our facility likewise identified differentially expressed vesicular-related proteins in rat hippocampus following stress exposure (20).…”

Section: Discussionsupporting

confidence: 78%

Candidate Hippocampal Biomarkers of Susceptibility and Resilience to Stress in a Rat Model of Depression

Henningsen

Palmfeldt

Christiansen

et al. 2012

Molecular & Cellular Proteomics

105

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Susceptibility to stress plays a crucial role in the development of psychiatric disorders such as unipolar depression and post-traumatic stress disorder. In the present study the chronic mild stress rat model of depression was used to reveal stress-susceptible and stress-resilient rats. Largescale proteomics was used to map hippocampal protein alterations in different stress states. Membrane proteins were successfully captured by two-phase separation and peptide based proteomics. Using iTRAQ labeling coupled with mass spectrometry, more than 2000 proteins were quantified and 73 proteins were found to be differentially expressed. Stress susceptibility was associated with increased expression of a sodium-channel protein (SCN9A) currently investigated as a potential antidepressant target. Differential protein profiling also indicated stress susceptibility to be associated with deficits in synaptic vesicle release involving SNCA, SYN-1, and AP-3. Our results indicate that increased oxidative phosphorylation (COX5A, NDUFB7, NDUFS8, COX5B, and UQCRB) within the hippocampal CA regions is part of a stress-protection mechanism.

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Section: Discussionsupporting

confidence: 78%

Candidate Hippocampal Biomarkers of Susceptibility and Resilience to Stress in a Rat Model of Depression

Henningsen

Palmfeldt

Christiansen

et al. 2012

Molecular & Cellular Proteomics

105

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“…Anhedonia, one of the core symptoms of depression, has been widely used in rodent depression model to evaluation degree of depression and effect of antidepressant drug [36]. Reduced preference for sucrose solution over water is a widely used animal model of anhedonia.…”

Section: Discussionmentioning

confidence: 99%

Chronic Clomipramine Treatment Reverses Core Symptom of Depression in Subordinate Tree Shrews

et al. 2013

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Chronic stress is the major cause of clinical depression. The behavioral signs of depression, including anhedonia, learning and memory deficits, and sleep disruption, result from the damaging effects of stress hormones on specific neural pathways. The Chinese tree shrew (Tupaia belangeri chinensis) is an aggressive non-human primate with a hierarchical social structure that has become a well-established model of the behavioral, endocrine, and neurobiological changes associated with stress-induced depression. The tricyclic antidepressant clomipramine treats many of the core symptoms of depression in humans. To further test the validity of the tree shrew model of depression, we examined the effects of clomipramine on depression-like behaviors and physiological stress responses induced by social defeat in subordinate tree shrews. Social defeat led to weight loss, anhedonia (as measured by sucrose preference), unstable fluctuations in locomotor activity, sustained urinary cortisol elevation, irregular cortisol rhythms, and deficient hippocampal long-term potentiation (LTP). Clomipramine ameliorated anhedonia and irregular locomotor activity, and partially rescued the irregular cortisol rhythm. In contrast, weight loss increased, cortisol levels were even higher, and in vitro LTP was still impaired in the clomipramine treatment group. These results demonstrate the unique advantage of the tree shrew social defeat model of depression.

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“…4A). Alteration of G3PDH has been previously implicated in the hippocampus and prefrontal cortex of depression rat models (Mallei et al, 2011;Marais et al, 2009;Piubelli et al, 2011) and in treatment with antidepressants (Carboni et al, 2006;Kedracka-Krok et al, 2010). PK catalyzes the rate-determing step in glycolysis-the transfer of a phosphate group from phosphoenolpyruvate to ADP that yields one molecule of pyruvate and one molecule of ATP.…”

Section: Perturbation Of Carbohydrate Metabolismmentioning

confidence: 99%

Combined Metabolomics and Proteomics Analysis of Major Depression in an Animal Model: Perturbed Energy Metabolism in the Chronic Mild Stressed Rat Cerebellum

Shao

Chen

Fan

et al. 2015

OMICS: A Journal of Integrative Biology

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Major depressive disorder (MDD) is a highly prevalent, debilitating mental illness of importance for global health. However, its molecular pathophysiology remains poorly understood. Combined proteomics and metabolomics approaches should provide a comprehensive understanding of MDD's etiology. The present study reports novel ''-omics'' insights from a rodent model of MDD. Cerebellar samples from chronic mild stressed (CMS)-treated depressed rats and controls were compared with a focus on the differentially expressed proteins and metabolites using isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomics and gas chromotography/mass spectrometry (GC-MS) metabolomics techniques, respectively. The combined analyses found significant alterations associated with cerebellar energy metabolism, as indicated by (1) abnormal amino acid metabolism accompanied by corresponding metabolic enzymatic alterations and disturbed protein turnover, (2) increased glycolytic and tricarboxylic acid (TCA) cycle enzyme levels paralleled by changes in the concentrations of associated metabolites, and (3) perturbation of ATP biosynthesis through adenosine accompanied by perturbation of the mitochondrial respiratory chain. To the best of our knowledge, this study is the first to integrate proteomics and metabolomics analyses to examine the pathophysiological mechanism(s) underlying MDD in a CMS rodent model of depression. These results can offer important insights into the pathogenesis of MDD.

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Effect of chronic mild stress and imipramine on the proteome of the rat dentate gyrus

Cited by 29 publications

References 65 publications

Candidate Hippocampal Biomarkers of Susceptibility and Resilience to Stress in a Rat Model of Depression

Candidate Hippocampal Biomarkers of Susceptibility and Resilience to Stress in a Rat Model of Depression

Chronic Clomipramine Treatment Reverses Core Symptom of Depression in Subordinate Tree Shrews

Combined Metabolomics and Proteomics Analysis of Major Depression in an Animal Model: Perturbed Energy Metabolism in the Chronic Mild Stressed Rat Cerebellum

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