Effect of chronic ipsilateral or contralateral intrauterine infusion of prostaglandin E2 (PGE2) on luteal function of unilaterally ovariectomized ewes

Magness, Ronald R.; Huie, J.M.; Hoyer, Gifford L.; Huecksteadt, Thomas P.; Reynolds, Lawrence P.; Seperich, G. J.; Whysong, G.; Weems, C.W.

doi:10.1016/0161-4630(81)90071-9

Cited by 116 publications

(49 citation statements)

References 38 publications

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“…luteoprotective actions, for establishing pregnancy (Pratt et al 1977, Magness et al 1981. Furthermore, TNFa has been demonstrated to affect the length of the estrous cycle through controlling uterine PG production in the cow .…”

Section: Discussionmentioning

confidence: 99%

The role of glucocorticoid in the regulation of prostaglandin biosynthesis in non-pregnant bovine endometrium

Lee

Acosta

Tanikawa

et al. 2007

Journal of Endocrinology

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To determine whether glucocorticoids (GCs) play a role in regulating uterine function in cow, the present study examined the expression of mRNA encoding GC receptor (GC-R) a, 11b-hydroxysteroid dehydrogenase (11-HSD) type 1 and type 2, and the activity of 11-HSD1 in bovine endometrial tissue throughout the estrous cycle. We also studied the effects of cortisol on basal, oxytocin (OT)-and tumor necrosis factor-a (TNFa)-stimulated prostaglandin (PG) production. A quantitative real-time PCR analysis revealed that GC-Ra mRNA was expressed more strongly in the mid-luteal stage (days 8-12) than in the other stages. In contrast to GC-Ra mRNA expression, 11-HSD1 mRNA expression was greater in the follicular stage than in the other stages, whereas 11-HSD2 mRNA expression was lowest in the follicular stage. The activity of 11-HSD1 was greater in the follicular stage and estrus than in the other stages and was lowest in the mid-luteal stage. Cortisone was dosedependently converted to cortisol in the cultured endometrial tissue. Although cortisol did not affect either the basal or OT-stimulated production of PGs in the cultured epithelial cells, the production of PGs stimulated by TNFa in the stromal cells was suppressed by cortisol (P!0 . 05). Cortisol suppressed basal prostaglandin (PG)F2a without affecting basal PGE2 production in the stromal cells. The overall results suggest that the level of cortisol is locally regulated in bovine endometrium throughout the estrous cycle by 11-HSD1, and that cortisol could act as a luteoprotective factor by selectively suppressing luteolytic PGF2a production in bovine endometrium.

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Section: Discussionmentioning

confidence: 99%

The role of glucocorticoid in the regulation of prostaglandin biosynthesis in non-pregnant bovine endometrium

Lee

Acosta

Tanikawa

et al. 2007

Journal of Endocrinology

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“…Exogenous PGF 2␣ can be used to terminate pregnancy and to initiate a new oestrous cycle for synchronization of ovulation in cyclic animals (Horton and Poyser, 1976;McCracken et al, 1999). Although it is less clearly established, PGE 2 is thought to exert actions opposite to PGF 2␣ to favour establishment of pregnancy and may have a luteoprotective action that is either luteotrophic or antiluteolytic (Pratt et al, 1977;Magness et al, 1981). PGE 2 also modulates the immune system to prevent rejection of the embryo allograft (Lala, 1990).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the contribution of cyclooxygenase 1 and cyclooxygenase 2 to the production of PGE2 and PGF2 alpha in epithelial cells from bovine endometrium

Parent

Villeneuve²,

Fortier³

2003

Reproduction

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In ruminants, the production of prostaglandins by the endometrium is critical for recognition of pregnancy. In the absence of an embryonic signal, luteolytic pulses of PGF 2␣ are released by the uterus. In contrast, the presence of a viable conceptus reduces the production of PGF 2␣ relative to PGE 2 and prevents luteolysis through the release of trophoblastic interferon (IFN-). Initially, it was thought that epithelial and stromal endometrial cells were specialized in the production of a single type of prostaglandin. However, purified cell populations of both types of cell can produce PGF 2␣ and PGE 2 ; therefore, selective production of PGF 2␣ and PGE 2 must be regulated within each type of cell. Two distinct prostaglandin synthases, cyclooxygenase 1 and cyclooxygenase 2, are involved in prostaglandin production and each may catalyse the production of a different prostaglandin. This possibility was investigated in cultured epithelial cells from bovine endometrium. Cells were treated with oxytocin or arachidonic acid, and expression of cyclooxygenase 1 and cyclooxygenase 2 proteins was monitored over time and correlated with prostaglandin accumulation. Cells were also treated with increasing doses of inhibitors of cyclooxygenase 1 or cyclooxygenase 2 (nonsteroidal anti-inflammatory drugs; NSAIDs) with or without arachidonic acid or oxytocin: flurbiprofen (0-50 mol l −1 ) was used as a non-selective inhibitor; valeryl salicylate (0-500 mol l −1 ) was used as a cyclooxygenase 1 inhibitor and NS-398 (0-1 mol l −1 ) was used as a cyclooxygenase 2 inhibitor. After stimulation with arachidonic acid or oxytocin, prostaglandin production and expression of cyclooxygenase 2 protein were increased. All inhibitors were able to block basal and stimulated prostaglandin production. These results indicate that in endometrium most, if not all, prostaglandin production is probably processed through cyclooxygenase 2.

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“…However, Crister et al, (1980) performed their studies during the early luteal phase (between days 5 and 8). These (Kummerfeld et al, 1978 ;Ball, 1978 ;Humblot, 1981 Huie et al, 1981 ;Gimenez and Henricks, 1983). Moreover, it was found from a preliminary experiment (Dalla Porta and Humblot, 1983) that PGE 2 , administered by the same way as these conceptuses, was less potent than day-16 conceptuses in maintaining progesterone production by the corpus luteum.…”

mentioning

confidence: 98%

Effect of conceptus removal and intrauterine administration of conceptus tissue on luteal function in the cow

Humblot¹,

Porta²

1984

Reprod. Nutr. Dévelop.

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Summary. Two experiments were conducted to determine the day on which the bovine embryo first affects luteal function.Experiment I was to determine the sole effect of flushing the uterine lumen and involved 12 non-inseminated heifers flushed 9, 14 and 16 days after estrus (4 animals per group). The same procedure was used to remove conceptuses from 15 pregnant heifers on days 9 (n = 4), 14 (n = 5) and 16 (n = 7) after the onset of estrus. Progesterone concentrations were measured daily throughout the cycle corresponding to conceptus removal (or flushing alone) and throughout the preceding cycle. In non-inseminated animals and when the conceptuses were removed on days 9 or 14, neither the progesterone pattern nor the inter-estrous interval was altered. By contrast, when the embryo was removed on day 16, the time of luteolysis was delayed by 4 to 7 days and the heifers were observed to be in estrus 26 to 29 days after Al.In experiment 2, 21 virgin heifers were distributed over four treatment groups. Animals in groups 2 to 4 received the following treatments twice a day between day 15 and day 19 of the estrous cycle in the horn ipsilateral to the corpus luteum : group 2 (n = 3), 0,25 ml saline ; group 3 (n = 3), two 12-day-old conceptuses ; group 4 (n = 5), one 16-day-old conceptus. Progesterone concentrations were determined from day 13 of the cycle to 4 days after the following estrus. No antiluteolytic effect was found with intrauterine administration of either saline alone or day-12 embryos. In contrast, administration of day-16 conceptuses through the cervix (group 4) lengthened the estrous cycle by 6 to 7 days.The results of these experiments suggest that the antiluteolytic and/or luteotropic factor(s) originating from the conceptus is fully potent by day 16. Embryonic mortality occuring on day 16 or later may induce an extension of corpus luteum lifespan.Introduction.

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Effect of chronic ipsilateral or contralateral intrauterine infusion of prostaglandin E2 (PGE2) on luteal function of unilaterally ovariectomized ewes

Cited by 116 publications

References 38 publications

The role of glucocorticoid in the regulation of prostaglandin biosynthesis in non-pregnant bovine endometrium

The role of glucocorticoid in the regulation of prostaglandin biosynthesis in non-pregnant bovine endometrium

Evaluation of the contribution of cyclooxygenase 1 and cyclooxygenase 2 to the production of PGE2 and PGF2 alpha in epithelial cells from bovine endometrium

Effect of conceptus removal and intrauterine administration of conceptus tissue on luteal function in the cow

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