Effect of Chronic Bladder Outlet Obstruction on Blood Flow of the Rabbit Bladder

Schröder, Annette; Chichester, Paul; Kogan, Barry A.; Longhurst, Penelope A.; Lieb, Jeremy; Das, Anupam; Levin, Robert M.

doi:10.1097/00005392-200102000-00087

Cited by 119 publications

(79 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…[22][23][24][25][26][27] The expression of angiostatic factors, such as Endostatin XV, due to the constantly increased collagen deposition and subsequent fibrosis, results in inhibition of further angiogenesis, decreasing blood flow, continuing ischemia injury and gradual progression to end-stage decompensation. [28][29][30] During the compensation phase, an increased expression of hypoxia inducible factor-a (HIF-a) is observed (Fig. 2a).…”

Section: Adjustment To Bladder Wall Ischemiamentioning

confidence: 99%

See 1 more Smart Citation

Obstruction-induced alterations within the urinary bladder and their role in the pathophysiology of lower urinary tract symptomatology.

Komninos

Mitsogiannis

2014

CUAJ

View full text Add to dashboard Cite

Cite as: Can Urol Assoc J 2014;8(7-8) AbstractBenign prostatic hyperplasia (BPH) is considered a frequent cause of bladder outlet obstruction (BOO) and lower urinary tract symptoms. This review addresses the bladder response to BOO and focuses on the alterations and biochemical adaptability of the bladder wall in the presence of hypoxia. A literature review of published articles has been performed, including both in vivo and in vitro studies on human and animal tissue.

show abstract

Section: Adjustment To Bladder Wall Ischemiamentioning

confidence: 99%

“…51 Animal studies in decompensated bladders showed that the severity of contractile dysfunction was correlated with the magnitude of the decrease in blood flow to the muscle compartment, supporting the hypothesis that ischemia is an etiological factor for bladder decompensation after BOO. 29 …”

Section: Pathophysiology Of Lutsmentioning

confidence: 99%

Obstruction-induced alterations within the urinary bladder and their role in the pathophysiology of lower urinary tract symptomatology.

Komninos

Mitsogiannis

2014

CUAJ

View full text Add to dashboard Cite

show abstract

“…An initial tension of 1.0 g was placed on each strip and was equilibrated for at least 30 minutes. At the end of the preincubation period, each strip was stimulated by electrical FS with 20-second trains of 80-V, 1-ms pulses at three frequencies (2,8, and 32 Hz) with 3-minutes interval between stimulus trains. Each strip was then stimulated with carbachol (20 μM), washed three times with fresh oxygenated buffer at 10-minute intervals, and stimulated with KCl (120 mM) followed by an additional three washes.…”

Section: Tissue Preparation and Equilibration In Normal Oxygenated Tymentioning

confidence: 99%

Effect of Co-enzyme Q10 and Alpha-Lipoic Acid on Response of Rabbit Urinary Bladder to Repetitive Stimulation and In Vitro Ischemia

Lin

Rehfuss

Schuler

et al. 2008

Urology

Self Cite

View full text Add to dashboard Cite

Objectives-The objective of these studies was to determine the efficacy of coenzyme Q10 (CoQ 10) and α-lipoic acid (α-LA), either alone or in combination, to protect the contractile responses of the rabbit urinary bladder from damage caused by repetitive stimulation in the presence or absence of in vitro ischemia.Methods-Four groups of New Zealand White rabbits (N=4 per group) were treated with vehicle (group 1), CoQ 10 (group 2), α-LA (group 3), or CoQ 10 + α-LA (group 4) for 2 weeks. At the end of the treatment period, eight longitudinal strips from each rabbit bladder body were placed in oxygenated Tyrode's solution with glucose (normal physiological medium). Strips were stimulated by field stimulation (FS), carbachol, and KCl, and the responses were recorded. One half the strips were switched for 1 hour to Tyrode's with no glucose equilibrated with nitrogen (ischemia medium). Simultaneously, all strips were subjected to 1 h of repetitive FS (RS) followed by 1 h of recovery in normal physiological medium and the responses to all stimuli were recorded again.Results-CoQ 10 showed no protective effect. α-LA resulted in increased contractile responses of the control bladder and showed a moderate protective effect for all forms of stimulation. The combination however, showed a significantly greater increased in the contraction of control bladder and a greater protective effect than α-LA alone Conclusion-The combination of α-LA and CoQ10 treatment both enhanced contractile response in normal medium, and diminished contractile dysfunction induced by RS and ischemia.

show abstract

“…Recent evidence has demonstrated that ischemia followed by reperfusion are major etiological factors in obstructive bladder dysfunction (3,4). Specifically, blood flow to the bladder's smooth muscle and mucosa decrease with obstructive Basic and Translational Urology doi: 10.1590/S1677-55382010000600014 dysfunction resulting in tissue hypoxia, increased free radical generation, decreased contraction, denervation, and mitochondrial dysfunction (5)(6)(7).…”

Section: Introductionmentioning

confidence: 99%

Effect of hydrogen peroxide on rabbit urinary bladder citrate synthase activity in the presence and absence of a grape suspension

et al. 2010

Self Cite

View full text Add to dashboard Cite

Purpose: The etiology of obstructive bladder dysfunction includes free radical damage to mitochondria. Feeding rabbits a standardized grape suspension protects the ability of the bladder to contract and empty in part by preventing mitochondrial damage, thus maintaining smooth muscle and mucosal metabolism. The objective of the current study is to determine the direct effect of this grape suspension on the response of mitochondria to the oxidative effects of hydrogen peroxide. Materials and Methods: Six male rabbits were anesthetized with sodium pentobarbital and the bladders excised. Four full thickness strips were obtained for contractile studies and the balance separated into smooth muscle and mucosa compartments by blunt dissection. The effect of hydrogen peroxide on the contractile response to field stimulation was quantitated. Each tissue was homogenized and the effects of increasing concentrations of hydrogen peroxide in the presence and absence of grape suspension on citrate synthase activity was determined. Results: Citrate synthase activity was significantly higher in the mucosa than in the muscle. The grape suspension had no effect on control citrate synthase activity. However, the grape suspension provided significant protection of both smooth muscle and mucosal citrate synthase activity. Conclusions: These studies support the conclusion that the grape suspension provides direct protection of mitochondrial function.

show abstract

Effect of Chronic Bladder Outlet Obstruction on Blood Flow of the Rabbit Bladder

Cited by 119 publications

References 30 publications

Obstruction-induced alterations within the urinary bladder and their role in the pathophysiology of lower urinary tract symptomatology.

Obstruction-induced alterations within the urinary bladder and their role in the pathophysiology of lower urinary tract symptomatology.

Effect of Co-enzyme Q10 and Alpha-Lipoic Acid on Response of Rabbit Urinary Bladder to Repetitive Stimulation and In Vitro Ischemia

Effect of hydrogen peroxide on rabbit urinary bladder citrate synthase activity in the presence and absence of a grape suspension

Contact Info

Product

Resources

About