Effect Of Chronic Administration Of Hexane Extract Of <i>Byrsonima Crassifolia</i> Seed On Β-Cell And Pancreatic Oxidative Parameters In Streptozotocin-Induced Diabetic Rat

Abstract

Background: In Mexican traditional medicine, the seeds of Byrsonima crassifolia have been used in the treatment of diabetes, rheumatism and for wound healing. The aim was to evaluate the effect of seeds of B. crassifolia on insulin release from the pancreatic beta cells in streptozotocininduced diabetic rats. Materials and Methods:In the present study, we evaluated the beneficial effect of seeds B. crassifolia streptozotocin-induced type 1 diabetic rats. Insulin level; insulin sensitivity index, insulin content in pancreas, malonaldehyde, nitric oxide contents, oxidative stress parameters were assayed. Serum glucose levels were determined by the glucose oxidase method. To determine the insulin releasing activity, after extract treatment, the pancreas was excised. Pancreatic sections were processed for examination of insulin-releasing activity using an imunocytochemistry kit. Results: Administration of the hexane extract (200 and 400 mg/kg), exhibited a significant reduction in serum glucose. Administration of streptozotocin decreased the number of beta cells with insulin secretory activity in comparison with intact rats; but treatment with the B. crassifolia seed extract increased significantly the activity of the beta cells in comparison with the diabetic control rats. The extract decreased serum glucose in streptozotocin-induced diabetic rats and increased insulin release from the beta cells of the pancreas. Conclusion: These finding suggest that B. crassifolia seed has beneficial effect for diabetes through decreasing blood glucose and lipid levels, increasing insulin sensitivity index and insulin content, up-regulating antioxidant enzyme activity and decreasing lipid peroxidation.

In Vitro Testing of Crude Natural Plant Extracts from Costa Rica for Their Ability to Boost Innate Immune Cells against Staphylococcus aureus

“…All plant extracts are from plants known as traditional medicinal plants: BYCRBA ( Byrsonima crassifolia ) was shown to act as an antidepressant with additional anti-inflammatory activity and gastro-protective effects [ 15 , 16 ]. The seed of BYCRBA affects diabetes as it decreases blood glucose [ 17 ]. Interestingly, the ethyl acetate of roots from BYCRBA showed antimicrobial effect against Klebsiella pneumoniae , Pseudomonas aeruginosa , Salmonella typhi , Shigella flexnari , S. aureus , S. epidermis , Streptococcus pneumoniae and Micrococcus luteus [ 18 ].…”

Therapeutic Role of Nitric Oxide in Diabetic Wound Healing: A Systematic Review

“…Byrsonima crassifolia seed showed antidiabetics and wound healing properties. It is evaluated in STZ-induce type-1 diabetic rats and parameters were assayed like insulin level in pancreas, nitric oxide contents and oxidative stress [23].…”

The Effects of Anacyclus pyrethrum Alcoholic Root Extract on FSH, LH, Testosterone and Sperm Count in Diabetic Male Rats

“…Type I diabetes was induced in overnight fasted male rat groups (DC, P, DA1, DA2 and DA3) by a single intraperitoneal injection of STZ, at a dose of 60mg/kg. STZ was purchased from Sigma Corporation and before using was immediately dissolved in citrate buffer (pH = 4.5) (27). All of the test animals were diabetic and showed diabetic symptoms by polydipsia, polyuria, and polyphagia.…”