Effect of chromium on the fatty acid composition of two strains of Euglena gracilis

Rocchetta, Iara; Mazzuca, Marcia; Conforti, Visitación; Ruiz, Laura; Balzaretti, V.; Molina, Marı́a del Carmen

doi:10.1016/j.envpol.2005.08.035

Cited by 117 publications

(56 citation statements)

References 31 publications

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“…Their findings can be used to explain the MUFA increase and PUFA decrease in cultures under N limitation in our study. Our finding that PUFA contents are significantly affected and their levels reduced in the presence of trace metals is also in accordance with other studies (Rocchetta et al, 2006;Pinto et al, 2011). The fatty acid 18:2(n-6) can be used as a nutrient stress indicator in C. vulgaris because its concentration increases under N limitation and Cd stress, unlike other PUFAs.…”

Section: Discussionsupporting

confidence: 80%

“…Pinto et al (2011) showed that SAFA and MUFA content of Gracilaria tenuistipitata increased with increasing Cd concentration, which is in agreement with our study showing increased SAFA and MUFA production by C. vulgaris in the presence of Cd. The accumulation of these fatty acids groups is thought to be dependent on oxidative stress and the production of reactive oxygen species (Pinto et al, 2003(Pinto et al, , 2011Rocchetta et al, 2006). Griffith et al (2012) reported that C. vulgaris responds to N limitation by increasing its SAFA and MUFA and decreasing its PUFA content.…”

Section: Discussionmentioning

confidence: 99%

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Effects of cadmium and nitrogen on lipid composition of Chlorella vulgaris (Trebouxiophyceae, Chlorophyta)

Chia

Lombardi

Melão

et al. 2013

European Journal of Phycology

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Investigations were carried out to evaluate lipid class and fatty acid composition of Chlorella vulgaris in relation to several combinations of cadmium (2 Â 10 -8 ; 10 À7 M Cd) and nitrogen (2.9 Â 10 À6 to 1.1 Â 10 À3 M N) concentrations. Triacylglycerols (TAG), acetone mobile polar lipids (AMPL) and phospholipids (PL) were the major lipid classes of C. vulgaris under all the tested conditions. TAG was the lipid class accumulated in the highest amount in N-limited C. vulgaris. The AMPL lipid class was lowest at the lowest N and highest Cd treatment. High proportions of saturated fatty acids (SAFA) and monounsaturated fatty acids (MUFA) in the total lipid extracts were recorded under N limitation in the presence of Cd. 16:1(n-11) was an exception among the MUFA as its levels decreased at low N. Total polyunsaturated FA (PUFA), !3 PUFA levels, and PUFA:SAFA ratios increased with increasing N concentrations, but when exposed to Cd, their levels were significantly reduced. Unlike the other PUFA, 18:2(n-6) increased with increasing N limitation. Significant negative relationships between TAG, MUFA and SAFA with N were recorded. Cd had a positive correlation with TAG, while N was with AMPL, PL and PUFA. In conclusion, a combination of N limitation and Cd stress significantly altered the lipid composition of C. vulgaris, and N limitation had the most significant overall effect on lipid class and fatty acid composition of the microalga.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Effects of cadmium and nitrogen on lipid composition of Chlorella vulgaris (Trebouxiophyceae, Chlorophyta)

Chia

Lombardi

Melão

et al. 2013

European Journal of Phycology

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“…The mechanisms involve mostly oxidative stress and the production of reactive oxygen/ nitrogen species that lead to oxidation of lipids (Pinto et al, 2003b). As expected and supported by other experiments with cultures of algae (Collen et al, 2003;Okamoto et al, 2001;Rocchetta et al, 2006), PUFA are the fatty acids most affected and their levels are reduced in the presence of heavy metals. In our experiments, Cd 2+ proved to be more toxic than Cu 2+ and significantly decreased the concentration of PUFA.…”

Section: Resultssupporting

confidence: 60%

“…Heavy metals can change the lipid and fatty acid composition of algae (Rocchetta et al, 2006;Vavilin et al, 1998). The mechanisms involve mostly oxidative stress and the production of reactive oxygen/ nitrogen species that lead to oxidation of lipids (Pinto et al, 2003b).…”

Section: Resultsmentioning

confidence: 99%

Effects of heavy metals and light levels on the biosynthesis of carotenoids and fatty acids in the macroalgae Gracilaria tenuistipitata (var. liui Zhang & Xia)

Pinto¹,

Carvalho²,

Cardozo³

et al. 2011

Rev. bras. farmacogn.

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“…Algal cells exposed to contaminants may suffer serious morphological and biochemical alterations (Rocchetta et al, 2006). Dichloromethane and dichloroethane not only disrupt the growth of bacteria and plants, but also show broad toxicity in mammals.…”

Section: Discussionmentioning

confidence: 99%

Toxicological Responses ofChlorella vulgaristo Dichloromethane and Dichloroethane

Zhang

et al. 2014

Environmental Engineering Science

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The aim of this study was to evaluate the acute toxicity effects of dichloromethane and dichloroethane on Chlorella vulgaris at the physiological and molecular level. Data showed that the cell number, chlorophyll a, and total protein content gradually decreased with increasing dichloromethane and dichloroethane concentrations over a 96-h exposure. Lower doses of two organic solvents had stimulatory effects on catalase and superoxide dismutase activity. Malondialdehyde showed a concentration-dependent increase in response to dichloromethane and dichloroethane exposure. Electron microscopy also showed that there were some chloroplast abnormalities in response to different concentrations of dichloromethane and dichloroethane exposure. Real-time polymerase chain reaction assay demonstrated that dichloromethane and dichloroethane reduced the transcript abundance of psaB, whereas that of psbC changed depending on the toxicant after 24 h of exposure. Dichloromethane and dichloroethane affected the activity of antioxidant enzymes, disrupted the chloroplast ultrastructure, and reduced transcription of photosynthesis-related genes in C. vulgaris, leading to metabolic disruption and cell death.

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Effect of chromium on the fatty acid composition of two strains of Euglena gracilis

Cited by 117 publications

References 31 publications

Effects of cadmium and nitrogen on lipid composition of Chlorella vulgaris (Trebouxiophyceae, Chlorophyta)

Effects of cadmium and nitrogen on lipid composition of Chlorella vulgaris (Trebouxiophyceae, Chlorophyta)

Effects of heavy metals and light levels on the biosynthesis of carotenoids and fatty acids in the macroalgae Gracilaria tenuistipitata (var. liui Zhang & Xia)

Toxicological Responses ofChlorella vulgaristo Dichloromethane and Dichloroethane

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