Effect of chromium accumulation on photosynthetic pigments, oxidative stress defense system, nitrate reduction, proline level and eugenol content of Ocimum tenuiflorum L.

Rai, Vibhuti; Vajpayee, Poornima; Singh, Sunil Kumar; Mehrotra, Shanta

doi:10.1016/j.plantsci.2004.06.016

Cited by 309 publications

(165 citation statements)

References 34 publications

Supporting

Mentioning

130

Contrasting

Unclassified

Order By: Relevance

“…This result might be attributed to the relatively higher suitability of this clone in the stressful environment. Rai et al (2004) stated peroxidase which participate in lignin biosynthesis and might built up a physical barrier against stress response hazards. Soluble, apoplastic PRXs can scavenge H 2 O 2 cooperating with phenolics (Takahama and Oniki 2000).…”

Section: Resultsmentioning

confidence: 99%

RETRACTED ARTICLE: Antioxidants and ROS scavenging ability in ten Darjeeling tea clones may serve as markers for selection of potentially adapted clones against abiotic stress

Dasgupta

Biswas

Kumar

et al. 2013

Physiol Mol Biol Plants

View full text Add to dashboard Cite

Ten Darjeeling tea clones (BT15/263, RR17/144, B777, T253, B157, Sundaram, HV39, AV2, K1/1 and TTV1) were collected from the experimental garden of Darjeeling Tea Research and Development Centre, Kurseong. Total phenol, flavonoids and two antioxidating enzymes (peroxidase and superoxide dismutase) were estimated. The total phenol ranged between 241 and 28 GAE mg g −1 of leaf dry weight. The highest amount obtained in four clones, B15/263 (241.47), RR17/144 (221.2), B777 (154.54) and B157 (140.23 mg g −1 ). Flavonoids were estimated as Catechin equivalent (CE) and ranged between 56.88 and 20.81 CE mg g −1 leaf dry weight. Higher amounts occurred in BT15/263 (56.88 mg g −1 ), B777 (56.69) and RR17/144 (48.63). Antioxidant activities were measured following DPPH and ABTS free radicle scavenging procedures and the results were well according to total polyphenol content among the clones (in total phenols, ranges of correlation in DPPH assay were r 2 =0.990-0.989, p≤0.05; in flavonoids r 2 =0.954, p≤0.01-0.987, p≤0.05). Similarly, ABTS percent scavenging results were quiet significant. The IC 50 values were determined for both DPPH and ABTS assay. PAGE expressions of isoforms in two antioxidative enzymes and quantification of them also varied much among the investigated clones. The incidence of total phenols, flavonoids, PRX and SOD and ROS scavenging assay in in-situ condition, might be used as biochemical markers towards the superior adaptability against abiotic stress. In the present work, four clones (B15/263, B777, RR17/144 and B157) would be designated as comparatively better suited to the predicted abiotic stress.

show abstract

Section: Resultsmentioning

confidence: 99%

RETRACTED ARTICLE: Antioxidants and ROS scavenging ability in ten Darjeeling tea clones may serve as markers for selection of potentially adapted clones against abiotic stress

Dasgupta

Biswas

Kumar

et al. 2013

Physiol Mol Biol Plants

View full text Add to dashboard Cite

show abstract

“…Chromium accumulation in O. tenuiflorium leads to malondialdehyde production (a cytotoxic product of lipid peroxidation) through excessive generation of free radicals (Rai et al 2004). Early studies showed that copper can interact with a wide range of physiological and biochemical processes in plant species (Jouili & Ezzedine 2003;Draobzkiewicz et al 2004) by increasing the production of reactive oxygen species and free radicals.…”

Section: Introductionmentioning

confidence: 99%

Biochemical changes and adaptive strategies of plants under heavy metal stress

2011

View full text Add to dashboard Cite

Heavy metal contamination of soil, aqueous waste stream and ground water causes major environmental and human health problems. Heavy metals are major environmental pollutants when they are present in high concentration in soil and show potential toxic effects on growth and development in plants. Due to unabated, indiscriminate and uncontrolled discharge of hazardous chemicals including heavy metals into the environment, plant continuously have to face various environmental constraints. In plants, seed germination is the first exchange interface with the surrounding medium and has been considered as highly sensitive to environmental changes. One of the crucial events during seed germination entails mobilization of seed reserves which is indispensable for the growth of embryonic axis. But, metabolic alterations by heavy metal exposure are known to depress the mobilization and utilization of reserve food by affecting the activity of hydrolytic enzymes. Some plants possess a range of potential mechanisms that may be involved in the detoxification of heavy metals by which they manage to survive under metal stress. High tolerance to heavy metal toxicity could rely either on reduced uptake or increase planned internal sequestration which is manifested by an interaction between a genotype and its environment. Such mechanism involves the binding of heavy metals to cell wall, immobilization, exclusion of the plasma membrane, efflux of these toxic metal ions, reduction of heavy metal transport, compartmentalization and metal chelation by tonoplast located transporters and expression of more general stress response mechanisms such as stress proteins. It is important to understand the toxicity response of plant to heavy metals so that we can utilize appropriate plant species in the rehabilitation of contaminated areas. Therefore, in the present review attempts have been made to evaluate the effects of increasing level of heavy metal in soils on the key behavior of hydrolytic and nitrogen assimilation enzymes. Additionally, it also provides a broad overview of the strategies adopted by plants against heavy metal stress.

show abstract

“…The Pro content of plants was formerly considered to act as a response to climatic change (Treichel et al 1984) and heavy metal contamination (Rai et al 2004;Sharma et al 2005). Effects of organic pollutants such as pesticides on the free Pro content in Euonymus japonica also suggested a substantially increased free Pro content after pesticide application for 10 days (Qu et al 2006).…”

Section: Effects On Antioxidase and Critical Compounds At 72 Hmentioning

confidence: 99%

Rape (Brassica chinensis L.) seed germination, seedling growth, and physiology in soil polluted with di-n-butyl phthalate and bis(2-ethylhexyl) phthalate

Christie

Teng

et al. 2013

Environ Sci Pollut Res

View full text Add to dashboard Cite

Phthalic acid esters (PAEs) pollution in agricultural soils caused by widely employed plastic products is becoming more and more widespread in China. PAEs polluted soil can lead to phytotoxicity in higher plants and potential health risks to human being. We evaluated the individual toxicity of di-n-butyl phthalate (DnBP) and bis (2-ethylhexyl) phthalate (DEHP), two representative PAEs, to sown rape (Brassica chinensis L.) seeds within 72 h (as germination stage) and seedlings after germination for 14 days by monitoring responses and trends of different biological parameters. No significant effects of six concentrations of PAE ranging from 0 (not treated/NT) to 500 mg kg −1 on germination rate in soil were observed. However, root length, shoot length, and biomass (fresh weight) were inhibited by both pollutants (except root length and biomass under DEHP). Stimulatory effects of both target pollutants on malondialdehyde (MDA) content, superoxide dismutase (SODase) activity, ascorbate peroxidase (APXase) content, and polyphenoloxidase (PPOase) activity in shoots and roots (SODase activity in shoots excluded) were in the same trend with the promotion of proline (Pro) but differed with acetylcholinesterase activity (except in shoots under DnBP) for analyzed samples treated for 72 h and 14 days.Responses of representative storage compounds free amino acids (FAA) and total soluble sugar (TSS) under both PAEs were raised. Sensitivity of APXase and Pro in roots demonstrates their possibility in estimation of PAE phytotoxicity and the higher toxicity of DnBP, which has also been approved by the morphological photos of seedlings at day 14. Higher sensitivity of the roots was also observed. The recommended soil allowable concentration is 5 mg DnBPkgsoil for the development of rape. We still need to know the phytotoxicity of DEHP at whole seedling stage for both the growing and development; on the other hand, soil criteria for PAE compounds are urgently required in China.

show abstract

Effect of chromium accumulation on photosynthetic pigments, oxidative stress defense system, nitrate reduction, proline level and eugenol content of Ocimum tenuiflorum L.

Cited by 309 publications

References 34 publications

RETRACTED ARTICLE: Antioxidants and ROS scavenging ability in ten Darjeeling tea clones may serve as markers for selection of potentially adapted clones against abiotic stress

RETRACTED ARTICLE: Antioxidants and ROS scavenging ability in ten Darjeeling tea clones may serve as markers for selection of potentially adapted clones against abiotic stress

Biochemical changes and adaptive strategies of plants under heavy metal stress

Rape (Brassica chinensis L.) seed germination, seedling growth, and physiology in soil polluted with di-n-butyl phthalate and bis(2-ethylhexyl) phthalate

Contact Info

Product

Resources

About