Effect of cholesterol-loaded-cyclodextrin on sperm viability and acrosome reaction in boar semen cryopreservation

Lee, Yong-Seung; Lee, Seunghyung; Lee, Sang‐Hee; Yang, Boo‐Keun; Park, Choon‐Keun

doi:10.1016/j.anireprosci.2015.06.006

Cited by 31 publications

(26 citation statements)

References 36 publications

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“…However, cryopreserved sperm showed damage on a variety of ways like decrease in intracellular pH and cAMP and have been deemed to be more sensitive to capacitating agents [292]. In boar sperm, addition of MBCD enhanced sperm vitality [293]. However, in other hand, it hinted this technology may impair sperm capacitation.…”

Section: Capacitation Related To Assisted Reproductive Technology Andmentioning

confidence: 99%

Factors and pathways involved in capacitation: how are they regulated?

Jin

Yang

2016

Oncotarget

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In mammals, fertilization occurs via a comprehensive progression of events. Freshly ejaculated sperm have yet to acquire progressive motility or fertilization ability. They must first undergo a series of biochemical and physiological changes, collectively known as capacitation. Capacitation is a significant prerequisite to fertilization. During the process of capacitation, changes in membrane properties, intracellular ion concentration and the activities of enzymes, together with other protein modifications, induce multiple signaling events and pathways in defined media in vitro or in the female reproductive tract in vivo. These, in turn, stimulate the acrosome reaction and prepare spermatozoa for penetration of the egg zona pellucida prior to fertilization. In the present review, we conclude all mainstream factors and pathways regulate capacitation and highlight their crosstalk. We also summarize the relationship between capacitation and assisted reproductive technology or human disease. In the end, we sum up the open questions and future avenues in this field.

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Section: Capacitation Related To Assisted Reproductive Technology Andmentioning

confidence: 99%

Factors and pathways involved in capacitation: how are they regulated?

Jin

Yang

2016

Oncotarget

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“…These spermatozoa can better endure the cold shock, but their fertility rate still did not increase [104]. The RAMEB·cholesterol complex is also useful in the cryopreservation of the sperm of other mammal species, namely Markhoz (Angora) bucks (Capra hircus) [106], buffalo bull [107], and boar [108].…”

Section: Semen Cryopreservationmentioning

confidence: 99%

Cyclodextrins: Emerging Medicines of the New Millennium

2019

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Cyclodextrins, since their discovery in the late 19th century, were mainly regarded as excipients. Nevertheless, developments in cyclodextrin research have shown that some of these hosts can capture and include biomolecules, highlighting fatty acids and cholesterol, which implies that they are not inert and that their action may be used in specific medicinal purposes. The present review, centered on literature reports from the year 2000 until the present day, presents a comprehensive description of the known biological activities of cyclodextrins and their implications for medicinal applications. The paper is divided into two main sections, one devoted to the properties and applications of cyclodextrins as active pharmaceutical ingredients in a variety of pathologies, from infectious ailments to cardiovascular dysfunctions and metabolic diseases. The second section is dedicated to the use of cyclodextrins in a range of biomedical technologies.

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“…El agregado de ciclodextrinas cargadas con colesterol (CLC) aumentaría el contenido de colesterol del espermatozoide manteniéndose elevado aún luego de someterlos a un proceso de congelado y descongelado (Amorim et al 2009,Moce et al 2010a,Purdy and Graham 2004b. El tratamiento con CLC a los espermatozoides, previo a la congelación, ha demostrado mejorar la motilidad y viabilidad no sólo inmediatamente luego de post-descongelado sino también a lo largo del tiempo (Moce et al 2010b), aumentar el número de espermatozoides unidos a la zona pelúcida (ZP) y porcentaje de penetración en ovocitos (Moore et al 2005,Purdy and Graham 2004a e incrementar la producción de embriones in vitro (Lee et al 2015).…”

Section: Introductionunclassified

Efecto de la suplementación con ciclodextrinas cargadas con colesterol al semen sexado bovino post-descongelación para su uso en producción in vitro de embriones

Ferré¹,

Grötter

Fresno

et al. 2018

ARCH ZOOTEC

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Los espermatozoides bovinos presentan una bajo relación de colesterol/fosfolípidos en la membrana plasmática. Esta característica es importante ya que el colesterol juega un papel fundamental en el mantenimiento de la estructura y funcionalidad de la membrana espermática tras la congelación, y, por lo tanto, en la supervivencia del espermatozoide tras el proceso de criopreservación. El proceso de sexado podría agravar todavía aún más esta situación. El objetivo de este trabajo es estabilizar las membranas plasmáticas de espermatozoides sexados mediante el agregado de ciclodextrinas cargadas con colesterol (CLC) post-descongelado y consecuentemente, prolongar su funcionalidad, aumentar su capacidad de fecundar ovocitos in vitro y producción de embriones. Para ello se trataron espermatozoides sexados con CLC a diferentes concentraciones e incubados por 15 minutos antes de evaluar el patrón de motilidad mediante un sistema computarizado CASA y fertilizar ovocitos in vitro. Los espermatozoides tratados con 3 mg de metil-β-ciclodextrina saturada de colesterol cada 120x106 espermatozoides presentaron mejor motilidad y vigor y se mantuvieron elevados durante el tiempo analizado. La concentración de 3 mg de CLC también produjo mayor clivaje, MOR/eBL, BL y embriones totales.

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Effect of cholesterol-loaded-cyclodextrin on sperm viability and acrosome reaction in boar semen cryopreservation

Cited by 31 publications

References 36 publications

Factors and pathways involved in capacitation: how are they regulated?

Factors and pathways involved in capacitation: how are they regulated?

Cyclodextrins: Emerging Medicines of the New Millennium

Efecto de la suplementación con ciclodextrinas cargadas con colesterol al semen sexado bovino post-descongelación para su uso en producción in vitro de embriones

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