Effect of cholesterol-loaded cyclodextrin on cryosurvival and fertility of cryopreserved carp (Cyprinus carpio) sperm

Yıldız, Cengiz; Yavaş, İlker; Bozkurt, Yusuf; Aksoy, Melih

doi:10.1016/j.cryobiol.2015.01.009

Cited by 29 publications

(17 citation statements)

References 30 publications

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“…A significant protection was observed against in vitro induced oxidative stress in CLC treated sperm. It is well known that CLC treatment of sperm protects them against harsh influence of freezing and thawing procedure in different animal species [9,[13][14][15]24,26,28,30,33,35,46]. The low concentrations of ROS (Superoxide anion; O 2À and H 2 O 2 ) in semen initiate the sperm capacitation [6], whereas high concentrations of ROS reduce the acrosin activity [48], motility [40], acrosome reaction, fertilizing ability [16] and increase the DNA fragmentation [17].…”

Section: Discussionmentioning

confidence: 99%

“…The enrichment of CLC in sperm membrane increased the membrane fluidity during cooling and cryopreservation and, decreased upon thawing which enhance the cryosurvival rate [34]. Through this method, the sperm cryosurvival rate of large variety of species including bull [33], ram [24], buck [15], buffalo [35], bison [13], mouse [26], elephant [14], rabbit [28], donkey [30], fish [46] and camel [9] has dramatically been increased. However, some species' sperm like swine [45], equine [27], rooster [44] and feline [32] did not show any improvement in motility, viability and acrosome integrity except membrane integrity.…”

Section: Introductionmentioning

confidence: 99%

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Protective effect of cholesterol-loaded cyclodextrin pretreatment against hydrogen peroxide induced oxidative damage in ram sperm

et al. 2015

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Protective effect of cholesterol-loaded cyclodextrin pretreatment against hydrogen peroxide induced oxidative damage in ram sperm

et al. 2015

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“…Adicionalmente, o colesterol também promove à estabilização e modulação da fluidez da membrana a temperatura corpórea (Lee et al 2015, Yildiz et al 2015.…”

Section: Introductionunclassified

Colesterol carregado pela ciclodextrina sobre a criopreservação dos espermatozoides de garanhões da raça Nordestina

2018

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RESUMO: Nesta pesquisa avaliou-se o efeito do colesterol sobre o sêmen de garanhões da raça Nordestina sobre a qualidade espermática. Vinte ejaculados de dois garanhões foram diluídos com BotuSemen e colesterol carreado pela ciclodextrina (CCC) adicionado no sêmen: controle, 0,75mg de CCC e 1,0mg de CCC/120x106 sptz/mL, e incubado a 26°C/15min. O sêmen foi diluído 1:5 (v/v) com diluente Lactose-gema de ovo e resfriado a 5°C/2h, envasado em palhetas de 0,5mL, e acondicionado sob vapor de nitrogênio líquido, e depois imersos. As amostras foram descongeladas (37 °C/30s) e avaliadas. As variáveis foram avaliadas com ANOVA e teste de Tukey (P<0,05). A motilidade total e progressiva foi maior (P<0,05) no sêmen tratado com CCC comparado as amostras do grupo controle, e CCC promoveu maior percentual (P<0,05) de motilidade total e progressiva durante as 3 horas de incubação. A percentagem de espermatozoides com viabilidade e integridade foi maior (P<0,05) no sêmen tratado com CCC (81,47 e 86,07%) comparado ao controle (72,12 e 70,19%). O número de espermatozoides reativos ao teste hiposmótico foi maior (P<0,05) nas amostras de sêmen tratadas com CCC comparado ao controle. Adição de colesterol no sêmen de garanhões Nordestino melhora a qualidade espermática apos a criopreservação.

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“…In this stage, most of the HBSS was decanted from the eggs, and fertilization was carried out in dry Petri dishes 0 cm diameter . Fresh or thawed sperm was added over the eggs and gently mixed before activation with 20 mL of fertilization solution 3 g urea and g NaCl in L distilled water [30]. Following fertilization process, 2 mL embryo bufer medium EBM 3.7 mM NaCl, .…”

Section: Fertilization Experimentsmentioning

confidence: 99%

Cryopreservation of Nile Tilapia (Oreochromis niloticus) Sperm

Bozkurt¹,

Yavaş²

2016

Cryopreservation in Eukaryotes

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The main aim of this study is to determine the efect of the straw volume 0.2 vs. 0. mL on Nile tilapia sperm quality after cryopreservation. Sperm was frozen according to conventional slow freezing procedure and diluted at ratio of :3 with ionic extender containing 3 0 mM glucose and 30 mM Tris containing 0% dimethylacetamide. Diluted semen was equilibrated at °C for 0 min and drawn into 0.2 -mL or 0. -mL plastic straws and sealed with polyvinyl alcohol. Samples were frozen 3 cm above of the liquid nitrogen surface and exposed to the liquid nitrogen vapor ≈− 0°C for 0 min. After this, frozen sperm cells were kept into the liquid nitrogen container − °C . The frozen sperm in diferent volume of straws were thawed in a water bath at 30°C for 20 s 0.2 -mL straws or at 30°C for 30 s 0. -mL straws , respectively. Fertilization was conducted using × 0 spermatozoa/egg ratio with each straw type. The indings of the present study indicated that cryopreservation of sperm in glucose-Tris-based extender using 0. -mL straws improved post-thaw progressive motility, duration of progressive motility, and fertilization results P<0.0 . On the other hand, diferences in term of post-thaw cell viability was not signiicant among the treatments P>0.0 . In conclusion, our results suggest that Nile tilapia sperm can be successfully cryopreserved in Tris-based extenders supplemented with glucose containing 0% dimethylacetamide in 0. -mL straws.

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Effect of cholesterol-loaded cyclodextrin on cryosurvival and fertility of cryopreserved carp (Cyprinus carpio) sperm

Cited by 29 publications

References 30 publications

Protective effect of cholesterol-loaded cyclodextrin pretreatment against hydrogen peroxide induced oxidative damage in ram sperm

Protective effect of cholesterol-loaded cyclodextrin pretreatment against hydrogen peroxide induced oxidative damage in ram sperm

Colesterol carregado pela ciclodextrina sobre a criopreservação dos espermatozoides de garanhões da raça Nordestina

Cryopreservation of Nile Tilapia (Oreochromis niloticus) Sperm

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