Effect of chlorogenic acid on intestinal inflammation, antioxidant status, and microbial community of young hens challenged with acute heat stress

Abstract

Heat stress in poultry is deleterious to productive performance. Chlorogenic acid (CGA) exerts antibacterial, anti-inflammatory, and antioxidant properties. This study was conducted to evaluate the effects of dietary supplemental CGA on the intestinal health and cecal microbiota composition of young hens challenged with acute heat stress. 100-day-old Hy-line brown pullets were randomly divided into four groups.The control group (C) and heat stress group (HS) received a basal diet. HS + CGA 300 group and HS + CGA 600 group received a basal diet supplemented with 300-and 600-mg/kg CGA, respectively, for 2 weeks before heat stress exposure. Pullets of HS, HS + CGA 300 , and HS + CGA 600 group were exposed to 38 C for 4 h while the control group was maintained at 25 C. In this study, dietary CGA supplementation had effect on mitigate the decreased T-AOC and T-SOD activities and the increasing of IL-1β and TNFα induced by acute heat stress. Dietary supplementation with 600 mg/kg CGA had better effect on increasing the relative abundance of beneficial bacterial genera, such as Rikenellaceae RC9_gut_group, Ruminococcaceae UCG-005, and Christensenellaceae R-7_group, and deceasing bacteria genera involved in inflammation, such as Sutterella species. Therefore, CGA can ameliorate acute heat stress damage through suppressing inflammation and improved antioxidant capacity and cecal microbiota composition.

Effect of Dietary Chlorogenic Acid on Growth Performance, Antioxidant Function, and Immune Response of Broiler Breeders under Immune Stress and Stocking Density Stress

“…In the present study, CGA increased the GSH content in the intestines of immune-stressed broiler breeders, in agreement with Shi [ 30 ], who found that CGA significantly increased GSH levels in liver tissue and reduced oxidative stress. Several studies have noted that CGA significantly enhanced antioxidant activity in broilers [ 11 , 12 , 25 , 26 ] and pigs [ 13 , 31 ], which was in agreement with our findings. The antioxidant effects of CGA were potentially attributed to its chemical structure for direct scavenging of free radicals and activation of endogenous antioxidant defenses against radicals [ 32 ].…”

“…This is in agreement with the conclusions of many studies that CGA has anti-inflammatory activities [ 36 , 37 ]. Supplemental CGA in the feed exhibited significant anti-inflammatory effects by decreasing the expression of IL-1β and TNF-α in heat-stressed broilers [ 12 ], in broilers infected with Clostridium perfringens type A [ 26 ], and in IBV-infected chickens [ 38 ].…”

“…It is widespread in many plants and Chinese herbal medicines [ 9 ]. Phenolic acids have been widely used as feed additives in poultry production due to their potent antioxidant [ 10 , 11 , 12 ], anti-inflammatory [ 13 , 14 , 15 ], antibacterial [ 16 , 17 ], and antiviral [ 18 , 19 ] activities. Although reports concerning the usefulness of CGA in poultry production have steadily increased, there is still little known about its ability to modulate the immune response, antioxidant function, and growth performance of broiler breeders under immune stress and high stocking density stress.…”

The Biological Activity Mechanism of Chlorogenic Acid and Its Applications in Food Industry: A Review

“…Additionally, CGA is used in chicken feed to change the oxidative stress and damage of the intestinal brush border membrane when the ambient temperature is too high ( 134 ). Heat stress may accelerate the glycolysis and metabolism of broiler chickens after death, decreasing pH after death and an increase in meat cooking loss.…”

Chlorogenic acid improves glucose tolerance, lipid metabolism, inflammation and microbiota composition in diabetic db/db mice

“…CGA can decrease the abundance of Lachnospiraceae , Ruminococcaceae , Desulfovibrionaceae , and Erysipelotrichaceae and increase that of Lactobacillaceae and Bacteroidaceae ( 14 ). Significantly, CGA improved the abundance of beneficial bacteria, including Ruminococcaceae UCG-005, Akkermansia , Christensenellaceae R-7_group , and Rikenellaceae RC9_gut_group ( 15 ) and short-chain fatty acid producers such as Faecalibaculum , Romboutsia , Mucispirillum , and Dubosiella ( 16 ). Meanwhile, CGA reduced the abundance of bacteria involved in inflammation, such as Sutterella species and those that cause intestinal dysfunction, such as Escherichia coli and other LPS-producers ( 17 , 18 ).…”