The purpose of this study was to evaluate the effect of methyl formate-methyl acetate (MF-MA) surface treatment on the flexural strength between denture base and hard reline materials. 180 heat-cured acrylic denture base (Meliodent?) specimens were prepared according to ISO 20795-1 (2013) and divided into 18 groups with various autopolymerizing hard reline materials. Group I-III: relined with Unifast Trad?, Group IV-VI: relined with Kooliner? Group VII-IX: relined with Tokuyama? Rebase II Fast (without adhesive, with hardener), Group X-XII: relined with Tokuyama? Rebase II Fast (with adhesive and hardener), Group XIII-XV: relined with Tokuyama? Rebase II Fast (without adhesive and hardener), Group XVI-XVIII: relined with Tokuyama? Rebase II Fast (with adhesive, without hardener). �Group I, IV ,VII and� XIII were untreated surface (control groups), Group II, V, VIII and XIV were surface treated with methyl methacrylate (MMA) for 180 s and Group III, VI, IX and XV �were surface treated with MF-MA solution for 15 s. Group X and XVI were surface treated with the manufacturer adhesive, �Group XI and XVII were surface treated with MMA 180 s and the manufacturer adhesive, Group XII and XVIII were surface treated with MF-MA 15 s and the manufacturer adhesive. The flexural strength was measured using a Universal Testing Machine. The data were analyzed using two-way ANOVA (group I-IX) and one-way ANOVA (group I-XVIII). �If the significant differences in the groups were found, the mean flexural strengths of the groups were compared using Tukey's test at a 95 % confidence level. For Tokuyama? Rebase II Fast, the data were analyzed using three-way ANOVA (Hardener, Manufacturing Adhesive, Surface treatment). The reline material type and surface treatments significantly affected on the flexural strength (p<0.05). For each reline material, the flexural strength of the MF-MA treated group was significantly higher compared with that of the MMA treated group and the MMA treated group had higher flexural strength than the untreated group (p<0.05). �For Tokuyama? Rebase II Fast, the surface treatment and manufacturing adhesive affected on the flexural strength (p< 0.05), but the hardener did not affected on the flexural strength (p> 0.05). Groups of additional surface treatment (MMA, and MF-MA) after applied with the adhesive significantly increased the flexural strength compared with the groups with only using the manufacturing adhesive (p<0.05). For the same surface treatment, the flexural strength of Unifast Trad? was significantly higher compared with Kooliner? (p<0.05). The flexural strength of Kooliner? was higher than that of Tokuyama? Rebase II Fast (p<0.05). This study suggests the application of MF-MA solutions for 15 s before relining procedure can increase the flexural strength between denture base and hard reline materials.