Effect of chloramphenicol on the biosynthesis of DNA in X-irradiated Escherichia coli B

Miletić, B; Kućan, Željko; Drakulić, M.; Zajec, L

doi:10.1016/0006-291x(61)90216-9

Cited by 22 publications

(3 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An interesting aspect of chloramphenicol treatment is its enhancement of both the rate and extent of DNA degradation in the cell population during the postirradiation period, as determined by colorimetric assay (Miletic et al, 1961a). The data presented here failed to show any deterimental effect of chloramphenicol on the amount of RNA synthesized by irradiated cells.…”

Section: Discussioncontrasting

confidence: 53%

Synthesis of Ribonucleic Acid by X-Irradiated Bacteria

Frampton

1964

J Bacteriol

View full text Add to dashboard Cite

Synthesis of ribonucleic acid by X-irradiated bacteria. J. Bacteriol. 87:1369-1376. 1964.-Postirradiation synthesis of total ribonucleic acid (RNA) and of RNA components was measured after exposure of Escherichia coli B/r to X rays. Net synthesis of RNA measured by the orcinol reaction and by the incorporation of uridine-2-C'4 was depressed in irradiated cells, but paralleled the period of postirradiation growth

show abstract

Section: Discussioncontrasting

confidence: 53%

Synthesis of Ribonucleic Acid by X-Irradiated Bacteria

Frampton

1964

J Bacteriol

View full text Add to dashboard Cite

show abstract

“…An early observation of relevance to the present work was made by Miletic et al (1961). They observed that in the presence of chloramphenicol the amount of postirradiation DNA degradation was increased, suggesting an involvement of protein synthesis.…”

Section: Introductionmentioning

confidence: 57%

Induction of radioresistance in Escherichia coli

Pollard¹,

Achey²

1975

Biophysical Journal

View full text Add to dashboard Cite

The effect of prior treatment by inducing agents on the radioresistance of cells of Escherichia coli has been studied. In order to separate the induction process from the radiation-damage process, cells were first treated with inducing agents such as ultraviolet light, ionizing radiation, or nalidixic acid, allowed to become induced by incubation for 50 min and then given rifampin to prevent further induction. They were then tested for radiation sensitivity. It was found that all strains tested except recA-, lex-, and recB showed very apparent protection. Induction by UV had the most effect and by nalidixic acid the least. The time course of development of protection was observed in one case: it is 50% established in 15 min. The absence of effect in recA- and lex- is explainable by the fact that these cells cannot be induced, for example, for prophage or the inducible inhibitor of post-irradiation DNA degradation. We suggest that the inducible inhibitor of postirradiation DNA degradation is one factor in a recovery system possessed by E. coli cells.

show abstract

“…The work presented in this paper concerns induced inhibition of postirradiation DNA degradation and repair of single-strand breaks caused by ionizing radiation. Early observations by Miletic et al (1961Miletic et al ( , 1964 suggested the presence of the degradation inhibitor in irradiated cells. The work of Grady and Pollard (1968) confirmed these findings and suggested that radiation acts to cause the initiation of DNA degradation and to induce a factor controlling the amount of degradation as well.…”

mentioning

confidence: 99%

Relative rates of repair of single-strand breaks and postirradiation DNA degradation in normal and induced cells of Escherichia coli

Pollard¹,

Fugate²

1978

Biophysical Journal

View full text Add to dashboard Cite

Labeled DNA from irradiated Excherichia coli cells has been studied on an alkaline sucrose gradient without acid precipitation of the DNA. This enables the observation of both DNA repair and DNA degradation. The use of a predose of ultraviolet light (UV) causes induction of an inhibitor of postirradiation DNA degradation in lex+ strains. The effect of this induction on both the repair of single-strand breaks and DNA degradation has been followed in strains WU3610 (uvr+) and WU3610-89 (uvr-). The repair process is more rapid than the degradation, and when degradation is inhibited more repair is apparent. Cells that are lex- (Bs-1 and AB2474) cannot be induced for inhibition of degradation. Nevertheless, by observation at short times repair can be seen clearly. This repaired DNA is degraded, suggesting that the signal for DNA degradation is not a single-strand break.

show abstract

Effect of chloramphenicol on the biosynthesis of DNA in X-irradiated Escherichia coli B

Cited by 22 publications

References 6 publications

Synthesis of Ribonucleic Acid by X-Irradiated Bacteria

Synthesis of Ribonucleic Acid by X-Irradiated Bacteria

Induction of radioresistance in Escherichia coli

Relative rates of repair of single-strand breaks and postirradiation DNA degradation in normal and induced cells of Escherichia coli

Contact Info

Product

Resources

About