Effect of chitosan treatment on strawberry allergen-related gene expression during ripening stages

Petriccione, Milena; Mastrobuoni, Francesco; Zampella, Luigi; Nobis, Elvira; Capriolo, Giuseppe; Scortichini, M.

doi:10.1007/s13197-017-2554-3

Cited by 10 publications

(9 citation statements)

References 30 publications

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“…5A). In the previous research, the transcript level of Fra a 1.01 was correlated with maturing stage; the younger fruits expressed a higher amount of mRNA (Ishibashi et al, 2017;Petriccione et al, 2017). Meanwhile, the Fra a 1.01 protein accumulation level was constant during the ripening stages (Ishibashi et al, 2017).…”

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confidence: 75%

“…Previous studies have revealed that there are two putative low temperature responsive elements in the promoter region of gene07080, a Fra a 1.01 ortholog in F. vesca (Alison Dunn et al, 1998;Ishibashi et al, 2018), and one element in the Fra a 1.01 promoter in F. × ananassa (unpublished data). Additionally, the transcript levels of Fra a 1.01 were found to be high during the green stage and relatively low during the red stage (Ishibashi et al, 2017;Petriccione et al, 2017). We considered that the accumulation levels of Fra a 1.01 in winter may depend on regulation by cold stress at the early ripening stage.…”

Section: Effects Of Low Temperature On Fra a 1 Expressionmentioning

confidence: 99%

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Seasonal Variation of the Major Allergen Fra a 1 in Strawberry Fruit

et al. 2019

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Strawberry (Fragaria × ananassa) contains a major allergen, Fra a 1, which causes oral allergic syndrome. Fra a 1 is a PR-10 homolog that is regulated by environmental conditions. The allergenicity of fruit caused by Fra a 1 may depend on the genotype or growing conditions. We analyzed the Fra a 1.01 transcript levels and Fra a 1.01 protein levels in strawberry fruits of several genotypes across all seasons. In the preliminary rough screening, we selected the line WH1 bearing white fruit and the red-fruited cultivar 'Akihime'. Under the same environmental conditions, there was no significant difference in Fra a 1.01 levels between the two cultivars over several months, suggesting that receptacle color was not indicative of allergenicity caused by Fra a 1.01. Fruits cultivated under the same environmental conditions should be used for comparisons of the allergenicity among genotypes. Both 'Akihime' and WH1 accumulated significantly higher levels of Fra a 1.01 protein in winter than in spring. We investigated the effects of irradiation and low temperature as environmental factors controlling the accumulation of Fra a 1.01 in winter. A shading treatment on fruit did not significantly affect Fra a 1.01 protein accumulation in strawberry fruits. Regarding variations over time, the Fra a 1.01 protein content was higher in fruits harvested at midnight in January than in those harvested at other times and in other months. These findings suggested that the Fra a 1.01 protein accumulates in response to environmental factors such as cold stress.

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confidence: 75%

Section: Effects Of Low Temperature On Fra a 1 Expressionmentioning

confidence: 99%

Seasonal Variation of the Major Allergen Fra a 1 in Strawberry Fruit

et al. 2019

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“…Although a meta‐analysis of publicly available data, related to transcriptome investigations of plants defense priming, evidenced a common set of conserved transcriptional changes on plants upon stress conditions, (Baccelli, Benny, Caruso, & Martinelli, 2020), the detailed role of the chitosan in the induction of defense mechanisms has been shown for sweet oranges (Coqueiro et al., 2015) and strawberries (Landi et al., 2017). The most common approaches related to the study of enzyme activities (Adiletta, Zampella, Coletta, & Petriccione, 2019; Ali et al., 2014; Pasquariello et al., 2015; Shao et al., 2015; Wang & Gao, 2013) and the expression of individual genes (Chun & Chandrasekaran, 2019; Fooladi vanda, Shabani, & Razavizadeh, 2019; Landi et al., 2014; Ma et al., 2013; Petriccione et al., 2017) have been investigated, both of which are associated with reactive oxygen species, specific PR proteins, cell‐wall enzymes and secondary metabolites. Usually, these individual studies have shown wide variability associated with host fruit species, application methods and times of treatment.…”

Section: Discussionmentioning

confidence: 99%

Chitosan and postharvest decay of fresh fruit: Meta‐analysis of disease control and antimicrobial and eliciting activities

Rajestary

Landi

Romanazzi

2020

Comp Rev Food Sci Food Safe

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Consumers are increasingly aware of the importance of regular consumption of fresh fruit in their diet. Since fresh fruit are highly sensitive to postharvest decay, several investigations focused on the study natural compounds alternative to synthetic fungicides, to extend their shelf life. A long list of studies reported the effectiveness of the natural biopolymer chitosan in control of postharvest diseases of fresh fruit. However, these findings remain controversial, with many mixed claims in the literature. In this work, we used random‐effects meta‐analysis to investigate the effects of 1% chitosan on (a) postharvest decay incidence; (b) mycelium growth of fungal pathogens Botrytis cinerea, Penicillium spp., Colletotrichum spp. and Alternaria spp.; and (c) phenylalanine ammonia‐lyase, chitinase and β‐1,3‐glucanase activities. Chitosan significantly reduced postharvest disease incidence (mean difference [MD], −30.22; p < 0.00001) and in vitro mycelium growth (MD, −54.32; p < 0.00001). For host defense responses, there were significantly increased activities of β‐1,3‐glucanase (MD, 115.06; p = 0.003) and chitinase (MD, 75.95; p < 0.0002). This systematic review contributes to confirm the multiple mechanisms of mechanisms of action of chitosan, which has unique properties in the natural compound panorama. Chitosan thus represents a model plant protection biopolymer for sustainable control of postharvest decay of fresh fruit.

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“…Fra a 1 shows various expression patterns at both the transcriptional and translational levels. In the fruit, each Fra a 1 paralog is differentially expressed at the ripening stage (Ishibashi et al, 2017;Petriccione et al, 2017). In vegetative organs, the expression levels of several paralogs can be induced by pathogen infection (Besbes et al, 2019;Toljamo et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…In vegetative organs, the expression levels of several paralogs can be induced by pathogen infection (Besbes et al, 2019;Toljamo et al, 2016). Several external stimuli induce Fra a 1 expression, including UV-C radiation, chitosan treatments, and achene removal (Ishibashi et al, 2017;Petriccione et al, 2017;Severo et al, 2015). Differences in Fra a 1 expression levels in varieties have been reported in some cultivation areas (Alm et al, 2007;Franz-Oberdorf et al, 2016); however, differences, as assessed by Fra a 1 protein accumulation levels, have not been documented in various production areas, including Japan (Franz-Oberdorf et al, 2017;Ishibashi et al, 2019b;Kurze et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Response of the Major Allergen Fra a 1.01 in Strawberry to Cold

et al. 2020

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Fra a 1 is a strawberry allergen that causes oral allergic syndrome. Fra a 1.01 is a major isoform that accumulates abundantly in fruits during the winter season. Here, we tested the hypothesis that Fra a 1.01 responds to environmental factors, such as cold stress. We analyzed transcriptional and translational levels of Fra a 1.01 in strawberry calli and organs under various cold conditions. First, we incubated strawberry (Fragaria × ananassa 'Akihime') calli and post-harvested fruits at low temperatures from several hours to days. Fra a 1.01 did not show significant differences in either gene expression or protein accumulation levels, suggesting that short-term cold treatments did not affect Fra a 1.01 expression. Second, we exposed whole plants to low temperature conditions for ~28 days. Under conditions below 10°C, Fra a 1.01 transcripts were induced gradually throughout the cold treatment (crown and root), or from 2 days to the last day (leaf and fruit). The Fra a 1.01 protein remarkably accumulated in crowns and slightly in fruits after 28 days. Finally, the promoter region of Fra a 1.01e was analyzed to detect tissue-specific expression. The cloned and sequenced promoter included several cis-acting regulatory elements related to cold response. When the Fra a 1.01 promoter region was heterologously expressed in Arabidopsis, the promoter activities, as assessed by GUS staining, were observed mainly around the shoot apices and in roots. Thus, Fra a 1.01 was considered to be expressed in crowns and roots, and was additively induced by cold stress. These organ-specific expressions could be important in elucidating the mechanisms responsible for Fra a 1.01 protein's accumulation in fruits during the winter season.

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Effect of chitosan treatment on strawberry allergen-related gene expression during ripening stages

Cited by 10 publications

References 30 publications

Seasonal Variation of the Major Allergen Fra a 1 in Strawberry Fruit

Seasonal Variation of the Major Allergen Fra a 1 in Strawberry Fruit

Chitosan and postharvest decay of fresh fruit: Meta‐analysis of disease control and antimicrobial and eliciting activities

Response of the Major Allergen Fra a 1.01 in Strawberry to Cold

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