Effect of chitosan scaffold microstructure on mesenchymal stem cell chondrogenesis

Ragetly, Guillaume; Griffon, Dominique J.; Lee, Haebeom; Fredericks, L. Page; Gordon‐Evans, Wanda J.; Chung, Yong Sik

doi:10.1016/j.actbio.2009.10.040

Cited by 91 publications

(54 citation statements)

References 56 publications

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“…The cells can recognize their microenvironments, and they selectively activate or down-regulate genes and modify their phenotypes accordingly. [25][26][27] The scaffold microstructure can direct In this study, chitosan fabricated in the form of sponges and hydrogels is comprehensively investigated and compared as the scaffolds for supporting cartilage formation with chondrocytes. Both forms of the scaffold in this study were fabricated with the same batch of chitosan, thus eliminating chemical composition as a possible influence for the different results.…”

Section: Discussionmentioning

confidence: 99%

Cells Behave Distinctly Within Sponges and Hydrogels Due to Differences of Internal Structure

Zhang

Yang

et al. 2013

Tissue Engineering Part A

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Different forms of biomaterials, including microspheres, sponges, hydrogels, and nanofibers, have been broadly used in cartilage regeneration; however, effects of internal structures of the biomaterials on cells and chondrogenesis remain largely unexplored. We hypothesized that different internal structures of sponges and hydrogels led to phenotypic disparity of the cells and may lead to disparate chondrogenesis. In the current study, the chondrocytes in sponges and hydrogels of chitosan were compared with regard to cell distribution, morphology, gene expression, and production of extracellular matrix. The chondrocytes clustered or attached to the materials with spindle morphologies in the sponges, while they distributed evenly with spherical morphologies in the hydrogels. The chondrocytes proliferated faster with elevated gene expression of collagen type I and down-regulated gene expression of aggracan in sponges, when compared with those in the hydrogels. However, there was no significant difference of the expression of collagen type II between these two scaffolds. Excretion of both glycosaminoglycan (GAG) and collagen type II increased with time in vitro, but there was no significant difference between the sponges and the hydrogels. There was no significant difference in secretion of GAG and collagen type II in the two scaffolds, while the levels of collagen type I and collagen type X were much higher in sponges compared with those in hydrogels during an in vivo study. Though the chondrocytes displayed different phenotypes in the sponges and hydrogels, they led to comparable chondrogenesis. An optimized design of the biomaterials could further improve chondrogenesis through enhancing functionalities of the chondrocytes.

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Section: Discussionmentioning

confidence: 99%

Cells Behave Distinctly Within Sponges and Hydrogels Due to Differences of Internal Structure

Zhang

Yang

et al. 2013

Tissue Engineering Part A

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“…3 A wide variety of biomaterials have been investigated for scaffolds in musculoskeletal tissue engineering, including synthetic polymers such as polylactic acid and polyglycolic acid [4][5][6][7] and natural materials such as chitosan and collagen. [8][9][10][11][12] However, there are many limitations to the use of these biomaterials for tissue engineering applications including the toxicity of crosslinking agents 13 and photoinitiators, 14 along with inadequate strength, 15 and weakening of the biomaterial during degradation. 16,17 Hydrogels can (a) be injected into the transplantation area, (b) take the shape of the defect, and (c) gel at body temperature.…”

Section: Introductionmentioning

confidence: 99%

Optimizing gelling parameters of gellan gum for fibrocartilage tissue engineering

et al. 2011

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Gellan gum is an attractive biomaterial for fibrocartilage tissue engineering applications because it is cell compatible, can be injected into a defect, and gels at body temperature. However, the gelling parameters of gellan gum have not yet been fully optimized. The aim of this study was to investigate the mechanics, degradation, gelling temperature, and viscosity of low acyl and low/high acyl gellan gum blends. Dynamic mechanical analysis showed that increased concentrations of low acyl gellan gum resulted in increased stiffness and the addition of high acyl gellan gum resulted in greatly decreased stiffness. Degradation studies showed that low acyl gellan gum was more stable than low/high acyl gellan gum blends. Gelling temperature studies showed that increased concentrations of low acyl gellan gum and CaCl₂ increased gelling temperature and low acyl gellan gum concentrations below 2% (w/v) would be most suitable for cell encapsulation. Gellan gum blends were generally found to have a higher gelling temperature than low acyl gellan gum. Viscosity studies showed that increased concentrations of low acyl gellan gum increased viscosity. Our results suggest that 2% (w/v) low acyl gellan gum would have the most appropriate mechanics, degradation, and gelling temperature for use in fibrocartilage tissue engineering applications.

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“…Further, highly interconnected open porous structures often facilitate optimized mass transport and cell migration into the core of the scaffolds [6]. Micro fibers have high surface to volume ratio, which account for enhanced cell-material interaction [7] and encourage maturation of cells with improved matrix production [8].…”

Section: Introductionmentioning

confidence: 99%

Development of chitosan-tripolyphosphate non-woven fibrous scaffolds for tissue engineering application

Pati

Adhikari

Dhara

2012

J Mater Sci: Mater Med

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The fibrous scaffolds are promising for tissue engineering applications because of their close structural resemblance with native extracellular matrix. Additionally, the chemical composition of scaffold is also an important consideration as they have significant influences on modulating cell attachment, morphology and function. In this study, chitosan-tripolyphosphate (TPP) non-woven fibrous scaffolds were prepared through wetspinning process. Interestingly, at physiological pH these scaffolds release phosphate ions, which have significant influences on cellular function. For the first time, cell viability in presence of varying concentration of sodium TPP solution was analyzed and correlated with the phosphate release from the scaffolds during 30 days incubation period. In vitro degradation of the chitosan-TPP scaffolds was higher than chitosan scaffolds, which may be due to decrease in crystallinity as a result of instantaneous ionic cross-linking during fiber formation. The scaffolds with highly interconnected porous structure present a remarkable cytocompatibility for cell growing, and show a great potential for tissue engineering applications.

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Effect of chitosan scaffold microstructure on mesenchymal stem cell chondrogenesis

Cited by 91 publications

References 56 publications

Cells Behave Distinctly Within Sponges and Hydrogels Due to Differences of Internal Structure

Cells Behave Distinctly Within Sponges and Hydrogels Due to Differences of Internal Structure

Optimizing gelling parameters of gellan gum for fibrocartilage tissue engineering

Development of chitosan-tripolyphosphate non-woven fibrous scaffolds for tissue engineering application

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