Effect of Chitosan-N-Acetylcysteine Conjugate in a Mouse Model of Botulinum Toxin B–Induced Dry Eye

Hongyok, Teeravee; Chae, Jeesoo; Shin, Young Joo; Na, Daero; Li, Li; Chuck, Roy S.

doi:10.1001/archophthalmol.2009.52

Cited by 22 publications

(12 citation statements)

References 34 publications

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“…Via the introduced thiol groups, C-NAC binds electrostatically and chemically to negatively charged reactive mucins of the ocular surface, thereby producing a stable glycocalyx-like structure. These scaffolding properties of C-NAC may lead to an enhanced stability of the polymer-mucin network, resulting in a prolonged residence time [23, 24] and promoting corneal wound healing.…”

Section: Discussionmentioning

confidence: 99%

Effect of Topically Administered Chitosan-N-acetylcysteine on Corneal Wound Healing in a Rabbit Model

Fischak

Klaus

Werkmeister

et al. 2017

Journal of Ophthalmology

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Purpose The present study was performed to investigate the effect of topically administered chitosan-N-acetylcysteine (C-NAC) on corneal wound healing in a rabbit model. Methods A total of 20 New Zealand White rabbits were included in the randomized, masked, placebo-controlled experiment. A monocular epithelial debridement was induced by manual scraping under general anesthesia. Animals were randomized to receive either C-NAC two times daily or placebo. Monitoring of corneal wound healing was performed with ultra-high-resolution optical coherence tomography (OCT) and epithelial fluorescein staining. Measurements were done immediately after and up to 72 hours after wound induction. Results No difference in wound size was found immediately after surgical debridement between the C-NAC group and the placebo group. Wound healing was significantly faster in the C-NAC group compared to the placebo group (p < 0.01 for both methods). A good correlation was found between the OCT technique and the epithelial fluorescein staining in terms of wound size (r = 0.94). Conclusions Administration of C-NAC containing eye drops twice daily leads to a faster corneal wound healing in a rabbit model of corneal debridement as compared to placebo. Ultra-high-resolution OCT is considered a noninvasive, dye-free alternative to conventional fluorescein staining in assessing corneal wound healing also in humans.

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Section: Discussionmentioning

confidence: 99%

Effect of Topically Administered Chitosan-N-acetylcysteine on Corneal Wound Healing in a Rabbit Model

Fischak

Klaus

Werkmeister

et al. 2017

Journal of Ophthalmology

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“…Em se tratando da produção lacrimal, o grupo com formulação oftálmica (grupo AAP) apresentou melhor efeito com relação ao aumento da produção lacrimal, que está relacionada pela associação dos vários componentes integrantes dessa formulação, como o álcool polivinílico a 1,4%, agente umectante que estabiliza a película lacrimal e impede sua ruptura, incrementando a viscosidade , a acetilcisteína, que possui propriedade mucolítica, anticolagenolítica e antioxidante (Yalçin et al, 2002;Aldavood et al, 2003;Hongyok et al, 2009), e a pilocarpina, droga parassimpatomimética, que possui efeito lacrimoestimulante, porém pode induzir irritação ocular, caracterizada por hiperemia conjuntival e blefaroespasmo, que não foi observado no presente experimento, talvez devido à sua diluição na formulação oftálmica (Andrade, 2008). Em um estudo em cães, foi relatado que a pilocarpina colírio em várias concentrações (0,25; 1,0 ou 2,0%) não alterou significativamente a produção lacrimal, porém a frequência de instilação realizada neste estudo foi nos dias quatro, seis e oito, o que pode ser considerada baixa e ser responsável pelos resultados obtidos (Smith et al, 1994).…”

Section: Discussão E Conclusãounclassified

Comparação entre dois protocolos de tratamento de ceratoconjuntivite seca experimentalmente induzida em coelhos

Motta

Yamasaki

Sanches

et al. 2014

Arq. Bras. Med. Vet. Zootec.

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 RESUMOO objetivo deste estudo foi avaliar e comparar a eficácia de dois protocolos de tratamento de ceratoconjuntivite seca (CCS) experimentalmente induzida em coelhos: uma formulação oftálmica tópica composta por álcool polivinílico 1,4%, adicionado com acetilcisteína 10% e pilocarpina 1% (AAP), e outro protocolo com o uso do óleo de semente de linhaça (OL) tópico em forma de colírio, durante 12 semanas. Foram utilizados 15 coelhos machos, adultos, da raça Nova Zelândia, alocados aleatoriamente em três grupos: grupo C (controle), grupo AAP (formulação oftálmica) e grupo L (OL tópica). Os animais foram avaliados semanalmente pelo teste lacrimal de Schirmer, teste de fluoresceína e teste de Rosa Bengala; uma vez por mês, pelo exame de citologia esfoliativa ocular; ao final do experimento, pela análise histopatológica da córnea e conjuntiva. Os resultados demonstraram que houve um aumento maior na produção lacrimal quando utilizada a formulação oftálmica, e uma resolução mais rápida das úlceras de córnea, bem como diminuição no número de células desvitalizadas quando utilizado o óleo de semente de linhaça, além de aumento no número de células caliciformes em ambos os grupos de tratamento. A associação desses dois protocolos pode ser no futuro uma alternativa no tratamento da CCS.Palavras-chave: ceratoconjuntivite seca, álcool polivinílico 1,4%, acetilcisteína 10%, pilocarpina 1%, óleo de linhaça ABSTRACT The objective of this study was to evaluate and compare the effectiveness of two treatment protocol of experimentally induced keratoconjunctivitis sicca (KCS) in rabbits, a topical ophthalmic formulation composed by 1.4% povinilic alcohol added with 10% acetylcysteine and 1% pilocarpine (AAP) and another protocol with the topical use of the linseed seed oil (LO) in eye drop form for 12 weeks. Fifteen male New Zealand white rabbits were aleatory allocated in 3 groups: Group C (Control), Group AAP (ophthalmic formulation) and Group L (LO topical

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“…The number of blinks was measured three times and averaged. 21 The tear production test was performed using phenolsulfonphthalein-saturated cotton threads (PRT test; Nourishi Optical, Danyang City, China). PRTs were applied in the lateral canthus of eye, using forceps, for 30 seconds in an unanesthetized mouse.…”

Section: Clinical Evaluationmentioning

confidence: 99%

Rapamycin Rescues Endoplasmic Reticulum Stress–Induced Dry Eye Syndrome in Mice

Cho

Hwang

Shin

et al. 2019

Invest. Ophthalmol. Vis. Sci.

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mycin rescues endoplasmic reticulum stress-induced dry eye syndrome in mice. Invest Ophthalmol Vis Sci. 2019;60:1254-1264. https://doi.org/ 10.1167 PURPOSE. To investigate whether rapamycin protects tear production and the ocular surface during endoplasmic reticulum (ER) stress-induced dry eye syndrome in mice. METHODS.Tunicamycin was injected intraperitoneally in BALB/c mice without or with rapamycin (TM or RM5 group). Peritoneal injection of PBS performed in vehicle group. Group without injection served as control. Blinking rate, fluorescein staining score (FSS), and phenol red thread tear production test were measured at 4 days, 1 week, and 2 weeks after treatment. Levels of inflammatory and angiogenic cytokines were measured by ELISA. RESULTS.Blinking rate and FSS were elevated, and tear production was decreased in TM group compared with controls (P < 0.05 for all), which was ameliorated by rapamycin at 1 and 2 weeks. Levels of inflammatory and angiogenic cytokines in the cornea and lacrimal glands were higher in the TM group than controls, and lower in the RM5 group than the TM group at 1 and 2 weeks (P < 0.05 for all).CONCLUSION. Rapamycin protected tear production and the ocular surface against this dry eye syndrome by ameliorating ER stress-induced vascular damage and inflammation of lacrimal glands and the ocular surface.

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Effect of Chitosan-N-Acetylcysteine Conjugate in a Mouse Model of Botulinum Toxin B–Induced Dry Eye

Cited by 22 publications

References 34 publications

Effect of Topically Administered Chitosan-N-acetylcysteine on Corneal Wound Healing in a Rabbit Model

Effect of Topically Administered Chitosan-N-acetylcysteine on Corneal Wound Healing in a Rabbit Model

Comparação entre dois protocolos de tratamento de ceratoconjuntivite seca experimentalmente induzida em coelhos

Rapamycin Rescues Endoplasmic Reticulum Stress–Induced Dry Eye Syndrome in Mice

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