Effect of chitosan derivatives on the reproduction of coliphages T2 and T7

Kochkina, Z. M.; Chirkov, Sergei

doi:10.1007/bf02756200

Cited by 13 publications

(9 citation statements)

References 5 publications

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“…They found increasing inhibition of the virus with increasing concentrations and complete inhibition was obtained at 0.01 % level. A study with coliphages showed that increases in chitosan, chitosan acetate, and chitosan hydrochloride concentrations from 0.00005 to 0.01 % caused an increase in inhibition of infection from \50 to 100 % against bacteriophage T2 and T7 (Kochkina and Chirkov 2000). Su et al (2009) also found that an increase in chitosan concentration from 0.175 to 0.7 % caused a significant increase in the reduction of FCV-F9 from 1.09 to 2.83 log PFU/mL by 53 kDa chitosan, and 0.44 to 1.44 log PFU/mL by 5 kDa chitosan.…”

Section: Effect Of Chitosan Concentration On Phi X174mentioning

confidence: 99%

“…In the previous study (Davis et al 2012), the mechanism of antiviral activity of chitosan was proposed along with reports of chitosan's ability to inactivate bacteriophages by binding to the negative charge of the viral capsid proteins causing viral structural damage, loss of tail fibers, and viral sheath damage, thereby exposing DNA, (Kochkina et al 2000;Su et al 2009), inhibiting infection (Kochkina et al 1995), and also replication (Chirkov 2002;Kochkina et al 2000). Chitosan is reported to penetrate the plant cell to induce resistance and thus exhibits indirect antiviral activity (Kulikov et al 2006).…”

Section: Effect Of Chitosan Concentration On Phi X174mentioning

confidence: 99%

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Enteric Viral Surrogate Reduction by Chitosan

et al. 2015

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Enteric viruses are a major problem in the food industry, especially as human noroviruses are the leading cause of nonbacterial gastroenteritis. Chitosan is known to be effective against some enteric viral surrogates, but more detailed studies are needed to determine the precise application variables. The main objective of this work was to determine the effect of increasing chitosan concentration (0.7-1.5% w/v) on the cultivable enteric viral surrogates, feline calicivirus (FCV-F9), murine norovirus (MNV-1), and bacteriophages (MS2 and phiX174) at 37 °C. Two chitosans (53 and 222 kDa) were dissolved in water (53 kDa) or 1% acetic acid (222 KDa) at 0.7-1.5%, and were then mixed with each virus to obtain a titer of ~5 log plaque-forming units (PFU)/mL. These mixtures were incubated for 3 h at 37 °C. Controls included untreated viruses in phosphate-buffered saline and viruses were enumerated by plaque assays. The 53 kDa chitosan at the concentrations tested reduced FCV-F9, MNV-1, MS2, and phi X174 by 2.6-2.9, 0.1-0.4, 2.6-2.8, and 0.7-0.9 log PFU/mL, respectively, while reduction by 222 kDa chitosan was 2.2-2.4, 0.8-1.0, 2.6-5.2, and 0.5-0.8 log PFU/mL, respectively. The 222 kDa chitosan at 1 and 0.7% w/v in acetic acid (pH 4.5) caused the greatest reductions of MS2 by 5.2 logs and 2.6 logs, respectively. Overall, chitosan treatments showed the greatest reduction of MS2, followed by FCV-F9, phi X174, and MNV-1. These two chitosans may contribute to the reduction of enteric viruses at the concentrations tested but would require use of other hurdles to eliminate food borne viruses.

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Section: Effect Of Chitosan Concentration On Phi X174mentioning

confidence: 99%

Section: Effect Of Chitosan Concentration On Phi X174mentioning

confidence: 99%

Enteric Viral Surrogate Reduction by Chitosan

et al. 2015

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“…lower DP and higher concentration had the best results whereas, in contrast, degree of deamination was not related to the inhibition property. Overall, low DP, high concentration, and deamination provided the best yield for Phage infection inhibitions, but it was speculated that inhibition was caused due to the antibacterial property of chitosan [ 45 ]. For phage viruses like MS2 and phi X 174, Davis et al, demonstrated the remarkable decrease in phage infection at different concentrations and molecular weights of chitosan for each of the phages in support of previous studies done before to emphasize the fact that overall charge on viral surface glycoproteins and charge on chitosan polymer affect the antiviral efficacy of chitosan.…”

Section: Chitosan and Its Derivatives In The Battleground Defeating mentioning

confidence: 99%

Underscoring the immense potential of chitosan in fighting a wide spectrum of viruses: A plausible molecule against SARS-CoV-2?

Sharma

Modak

Singh

et al. 2021

International Journal of Biological Macromolecules

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Chitosan is a deacetylated polycationic polysaccharide derived from chitin. It is structurally constituted of N -acetyl-D-glucosamine and β-(1-4)-linked D-glucosamine where acetyl groups are randomly distributed across the polymer. The parameters of deacetylation and depolymerization process greatly influence various physico-chemical properties of chitosan and thus, offer a great degree of manipulation to synthesize chitosan of interest for various industrial and biomedical applications. Chitosan and its various derivatives have been a potential molecule of investigation in the area of anti-microbials especially anti-fungal, anti-bacterial and antiviral. The current review predominantly highlights and discusses about the antiviral activities of chitosan and its various substituted derivatives against a wide spectrum of human, animal, plants and bacteriophage viruses. The extrinsic and intrinsic factors that affect antiviral efficacy of chitosan have also been talked about. With the rapid unfolding of COVID-19 pandemic across the globe, we look for chitosan as a plausible potent antiviral molecule for fighting this disease. Through this review, we present enough literature data supporting role of chitosan against different strains of SARS viruses and also chitosan targeting CD147 receptors, a novel route for invasion of SARS-CoV-2 into host cells. We speculate the possibility of using chitosan as potential molecule against SARS-CoV-2 virus.

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“…Anyonik kitosan türevlerinin antiviral etkilerinin düşük ve deasetilasyon derecesi yüksek kitosanların antiviral etkilerinin de yüksek olması kitosanın yapısında bulunan pozitif yüklü grupların antiviral aktiviteden sorumlu olduğunu göstermektedir [56]. Ayrıca % 50 deamine edilmiş kitosan türevlerinin antiviral etkilerini kaybetmeleri de pozitif yüklerin antiviral aktivite için önemini bir kez daha ortaya koymuştur [23].…”

Section: Antiviral Mekanizmaunclassified

Ki̇tosan Ve Anti̇mi̇krobi̇yal Özelli̇kleri̇

Yıldırım

Öncül²,

Yildirim

2016

Ömer Halisdemir Üniversitesi Mühendislik Bilimleri Dergisi

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ÖZKitosan, kitinin kısmen veya tamamen deastilasyonuyla elde edilen değişik moleküler ağrılıktaki bileşikler için kullanılan genel bir terimdir. Doğada yaygın ve bol miktarda bulunması, yüksek canlılara karşı toksik etki göstermemesi, mikroorganizmaları inhibe etmesi, biyolojik olarak parçalanabilmesi, biyo-uyumlu olması, kimyasal ve fiziksel özellikleri bakımından diğer birçok biyopolimere göre üstün özellikler göstermesi nedeniyle kitosan başta gıda olmak üzere su, ilaç, kozmetik, ziraat, tıp, kâğıt, tekstil, mikrobiyoloji, immünoloji, biyoteknoloji, ters osmoz membranları gibi birçok alanda kullanım olanağı bulmaktadır. Bu derleme, kitosanın antimikrobiyal aktivitesi ve etki mekanizması, antimikrobiyal aktivitesini etkileyen faktörler ve gıda endüstrisinde kullanımı ile ilgili bilgileri içermektedir.Anahtar Kelimeler: Kitosan, antimikrobiyal aktivite, etki mekanizması, gıda CHITOSAN AND ANTIMICROBIAL PROPERTIES ABSTRACTChitosan is a collective name used for a group of compounds having various molecular weights, which are produced from chitin by partially or fully de-acetylating. Because of its distinctive biological and physicochemical properties, including non-toxic, biocompatible, biodegradable, antimicrobial, antioxidant, anticancer and non-antigenic activities, chitosan has found many applications in different areas such as: food, water treatment, pharmaceutical, cosmetics, agriculture, biomedical, paper, textile, microbiology, immunology, biotechnology and reverse osmosis membranes. This review gives information about antimicrobial activity of chitosan, its mode of action against microorganisms, factors affecting its antimicrobial activity and its application in food industry.Keywords: Chitosan, antimicrobial activity, mode of action, food GİRİŞKitosan [(C 6 H 11 NO 4 ) n (C 8 H 13 NO 4 ) m ], ilk kez Bradconnot tarafından 1811 yılında tanımlanmış olan kitinin (β-1,4-poli-N-asetil-D-glukozamin) kısmen veya tamamen deasetilasyonu ile elde edilen antimikrobiyal özelliğe sahip bir heteropolisakkarittir [1,2]. Kitinin deasetilasyonu sonucu elde edildiği için kitosan, farklı oran ve sayıda 2-amino-2-deoksi-β-D-glukopiranoz (D-glukozamin, deasetile form, yaklaşık % 80) ve 2-asetamido-2-deoksi-β-D-glukopiranoz (N-asetil-D-glukozamin, asetile form, yaklaşık % 20) moleküllerinin β-(1,4) bağı ile bağlanması sonucu oluşan düz zincirli bir kopolimerdir. Bu iki molekülün sayısı ve dizi içerisindeki sırası

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Effect of chitosan derivatives on the reproduction of coliphages T2 and T7

Cited by 13 publications

References 5 publications

Enteric Viral Surrogate Reduction by Chitosan

Enteric Viral Surrogate Reduction by Chitosan

Underscoring the immense potential of chitosan in fighting a wide spectrum of viruses: A plausible molecule against SARS-CoV-2?

Ki̇tosan Ve Anti̇mi̇krobi̇yal Özelli̇kleri̇

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