Effect of Chitin Nanofibrils on Biocompatibility and Bioactivity of the Chitosan-Based Composite Film Matrix Intended for Tissue Engineering

Abstract

This paper discusses the mechanical and physicochemical properties of film matrices based on chitosan, as well as the possibility of optimizing these properties by adding chitin nanofibrils. It is shown that with the introduction of chitin nanofibrils as a filler, the mechanical stability of the composite materials increases. By varying the concentration of chitin nanofibrils, it is possible to obtain a spectrum of samples with different bioactive properties for the growth of human dermal fibroblasts. Film matrices based on the nanocomposite of chitosan and 5 wt % chitin nanofibrils have an optimal balance of mechanical and physicochemical properties and bioactivity in relation to the culture of human dermal fibroblasts.

Extraction and physicochemical characterization of chitin derived from the Asian hornet, <em>Vespa velutina</em> Lepeletier 1836 (Hym.: Vespidae)

“…One of the most commonly used series for the estimation of p (x) is the Schlömlich expansion, used with good results by Doyle. (19) Doyle considers it sufficient to take only the first two terms of each series. With what: (20) And found that for x> 20 and taking logarithms could express p (x) as:…”

“…The great variety of applications of chitosan in the field of biomaterials is due to its excellent properties when interacting with the human body: bioactivity [7], antimicrobial and antifungal activity [8,9], immunostimulation [10], chemotactic action, enzymatic biodegradability, mucoadhesion and epithelial permeability [11] which supports the adhesion and proliferation of different cell types [11][12][13]. With the above properties, CHI derivatives serve a broad range of applications and these materials have advanced at a dramatic pace into many fields, including skin, wound and burn management [14][15][16]; drug delivery and pharmaceutical applications [17][18]; tissue engineering [19][20][21]; dentistry [22][23][24]; plant science and agriculture [25,26]; veterinary science [27,28]; cosmetics and cosmeceutical [29,30]; food and nutraceuticals [31,32]; and paper industry [33], among others.…”

Engineered biomaterial strategies for controlling growth factors in tissue engineering

“…Numerous studies have shown that chitosan and chitin can be used in a variety of applications in tissue engineering (Yilgor et al., 2009 ; Liu et al., 2016 ; Pangon et al., 2016 ; Islam et al., 2017 ; Ahsan et al., 2018 ). They can be designed into a variety of structures such as gels, membranes nanofibers, nanoparticle and sponges (Singh et al., 2017 ; Smirnova et al., 2019 ; Zubillaga et al., 2020 ). In combination with properties such as its good biocompatibility, biodegradability, nontoxic, anti-inflammatory, adhesion, antibacterial and nerve protection, chitosan can be made into a suitable candidate for growth factors delivery (Rajam et al., 2011 ).…”

Creation of Ionically Crosslinked Tri-Layered Chitosan Membranes to Simulate Different Human Skin Properties

“…Until now, there have been different proposals of skin-like substitutes in an attempt to reproduce several aspects of real human skin, but, for many reasons, they are not considered as perfect human skin alternatives [ 1 ]. In most cases, living cells are cultured in an artificial scaffold, which means that the skin is difficult to manage and affects its costs and expiry date [ 5 , 6 ].…”