“…The majority of studies on generated temperatures have been conducted in vitro [6,[11][12][13][14][15] but also in vivo [16], ex vivo [17] and clinical [18,19] settings. The following fibres were used: 200, 242, 272, 365, and 550. studied lasers always operated in pulsed mode with power ratings ranging from 10 to 100 W. Laser activation times ranged from three to 120 s. Surgeons have utilized intelligent pumps [12,15,17], manual/infusion pumps [14,20], and gravity irrigation [6,11,12,16,20]. The applied flow rates ranged from 5 to 100 mL/min.…”