Effect of Chicken Antiserum and Tissue Extracts on the Oocysts, Sporozoites, and Merozoites of Eimeria tenella and E. acervulina

Herlich, H.

doi:10.2307/3276175

Cited by 14 publications

(7 citation statements)

References 5 publications

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“…With cecal contents some complement-mediated, lethal effects were manifested by the increased uptake of trypan blue staining. This is assumed to be an intermediate stage in sporozoite lysis, as has been described, mediated by serum antibodies together with complement [2,4,9,13; 301, seen in the present results with serum as a deformation or stumping of sporozoites associated with trypan blue staining. This is evidence that under conditions of high gut permeability, complement may be present and functionally active together with IgG in the gut lumen.…”

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confidence: 80%

Eimeria tenella: Local Antibodies and Interactions with the Sporozoite Surface

Trees¹,

Karim²,

McKellar³

et al. 1989

The Journal of Protozoology

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Using fixed sporozoites in a 3-layer immunofluorescence assay (TLIFA), class-specific, parasite-specific antibody responses in chicks to single-pulse infection with Eimeria tenella have been studied in gut contents and bile as well as plasma and feces. After infection with 10(3) oocysts, IgA antibody was first detected in the duodenal lumen, then in bile, plasma, cecum, and the distal small intestine. The kinetics of the bile IgA response correlated with that in plasma and peaked 9 days post-infection (d.p.i.); IgM was detected in gut contents and bile as well as plasma, and IgG was occasionally detected in gut contents, especially in the duodenum. In some experiments, IgA was detected in gut contents and bile to at least 21 d.p.i. Infection with 10(5) oocysts resulted in an earlier and increased response and relatively high IgG titers in cecal contents. Coproantibody was detected inconsistently and at low titer. When sporozoites that excysted in vitro were incubated in specific, antibody-positive (9 d.p.i.) cecal contents, some complement-mediated IgG-associated anti-sporozoite effects were observed; however, the major effect of cecal contents and the only effect of bile was a non-lethal agglutination of living sporozoites. By fractionation of cecal contents and immunoblotting this was confirmed to be IgA mediated; IgA antibodies in cecal contents and bile after infection were shown to bind to sporozoite membrane antigens by surface fluorescence as well as agglutination. Agglutination detected anti-sporozoite antibody in gut contents and bile up to 21 d.p.i., peaking between 7 and 13 d.p.i., corresponding with TLIFA results.(ABSTRACT TRUNCATED AT 250 WORDS)

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confidence: 80%

Eimeria tenella: Local Antibodies and Interactions with the Sporozoite Surface

Trees¹,

Karim²,

McKellar³

et al. 1989

The Journal of Protozoology

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“…Although the interaction of sporozoites of chicken coccidia with antibodies from various sources has been documented extensively, it is surprising that agglutination of sporozoites by immune sera has not been reported. However, sporozoite lysis (Long, Rose & Pierce 1963, Burns & Challey 1965, Speer, Wong & Schenkel 1983 immobiliza-tion (Herlich 1965) surface changes (Witlock & Danforth 1982, Speer et al 1985 and neutralization in uitro (Long & Rose 1972, Davis, Parry & Porter 1978, Danforth 1983 and in uiuo (Long et a1 1963, Herlich 1965, Rose 1974, Crane, Gnozzio & Murray 1985 have been observed. Since few quantitative techniques were used in sporozoite studies the biological significance of anti-sporozoite antibodies remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Eimeria tenella: quantitative in vitro and in vivo studies on the effects of mouse polyclonal and monoclonal antibodies on sporozoites

Crane

Norman

Gnozzio

et al. 1986

Parasite Immunology

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Murine, polyclonal and monoclonal antibodies, raised against sporozoites of Eimeria tenella, were tested for their ability to neutralize sporozoite infectivity in vitro and in vivo. Neutralization was effected via three mechanisms. Firstly, sporozoites fixed complement, at low titres, and lysis occurred by the alternative pathway of complement activation. Secondly, in the absence of complement activity, the murine heat-inactivated, hyperimmune antiserum neutralized sporozoites at relatively low titres. At high titres, even though sporozoites were agglutinated, neither the heat-inactivated hyperimmune antiserum nor the monoclonal antibody neutralized sporozoites. Finally, in the presence of complement and specific antibodies, at titres which by themselves would not neutralize sporozoites, neutralization was effected due to lysis via the classical pathway of complement activation.

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“…The effector mechanism(s) of protective immunity induced by live infection or by extract vaccine remains unclear, however. Although antibodies from various sources demonstrate a variety of antiparasitic activities, including sporozoite immobilization (9), sporozoite and merozoite agglutination and lysis (2,14), neutralization (1,2,9,14,15,22), and surface changes (31), the role of serum antibody in protective immunity is unclear (11,18,(23)(24)(25)28). Recently, emphasis has been directed towards cell-mediated effectors (11,24,25) or secretory antibody (immunoglobulin A [IgA]), which has been shown to neutralize sporozoites both in vitro and in vivo (5,6).…”

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confidence: 99%

Passive protection of chickens against Eimeria tenella infection by monoclonal antibody

et al. 1988

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Monoclonal antibodies reactive with the surface of Eimeria tenella sporozoites were produced in mice. This paper concerns one of these monoclonal antibodies, designated 1073.10, which agglutinated sporozoites in vitro and lysed the parasite in the presence of complement. This treatment neutralized sporozoite infections when the treated parasites were injected into the ceca of normal chickens. Passive transfer of ammonium sulfate-precipitated 1073.10 ascites fluid into 2- to 3-day-old or 3-week-old chickens conferred protection against challenge infection with E. tenella. These studies show that serum antibody may play a role in immunity to coccidiosis and that the sporozoite surface epitope recognized by 1073.10 is a possible vaccine candidate antigen.

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Effect of Chicken Antiserum and Tissue Extracts on the Oocysts, Sporozoites, and Merozoites of Eimeria tenella and E. acervulina

Cited by 14 publications

References 5 publications

Eimeria tenella: Local Antibodies and Interactions with the Sporozoite Surface

Eimeria tenella: Local Antibodies and Interactions with the Sporozoite Surface

Eimeria tenella: quantitative in vitro and in vivo studies on the effects of mouse polyclonal and monoclonal antibodies on sporozoites

Passive protection of chickens against Eimeria tenella infection by monoclonal antibody

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