Effect of chemical structural properties of chitooligosaccharides on the immune activity of macrophages

Jeong, Dae-Yong; Lee, Eung Take; Lee, Jisun; Shin, Dong-Joon; Lee, Yong Hyun; Park, Jae Kweon

doi:10.1007/s13233-023-00143-8

Cited by 3 publications

(1 citation statement)

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“…Cytokines have been studied to coordinate the immune response and mediate intracellular communication [36]. Moreover, macrophages secrete cytokines, such as TNF-α, IL-6, and IL-1β which play an important role in initiating the immune response [37]. As shown in Fig.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant and immunostimulatory effect of heat-treated paraprobiotics Latilactobacillus sakei KU15041 and Latilactobacillus curvatus KU15003

Hyun,

Woo,

Kim

et al. 2023

Preprint

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The lactic acid bacteria, including Latilactobacillus sakei and Latilactobacillus curvatus, have been widely studied for their preventive and therapeutic effects. In this study, the underlying mechanism of action for the antioxidant and immunostimulatory effects of two strains of heat-treated paraprobiotics was examined. Heat-treated Lact. sakei KU15041 and Lact. curvatus KU15003 showed higher radical scavenging activity in both the 2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assays than the commercial probiotic strain LGG. In addition, treatment with these two strains exhibited immunostimulatory effects in RAW 264.7 macrophages, with Lact. curvatus KU15003 showing a slightly higher effect. Additionally, they promoted phagocytosis and NO production in RAW 264.7 cells without any cytotoxicity. Moreover, the expression of tumor necrosis factor-α, interleukin (IL)-1β, and IL-6 was upregulated. These strains resulted in an increased expression of inducible nitric oxide synthase and cyclooxygenase-2. Moreover, the nuclear factor-κB and mitogen-activated protein kinase signaling pathways were stimulated by these strains. These findings suggest the potential of using Lact. sakei KU15041 and Lact. curvatus KU15003 in food or by themselves as probiotics with antioxidant and immune-enhancing properties.

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Section: Discussionmentioning

confidence: 99%