Effect of Chemical Cross-linking on Properties of Gelatin/Hyaluronic Acid Composite Hydrogels

Zhou, Zhi‐Hua; Yang, Zhaorong; Huang, Tianlong; Liu, Lihua; Liu, Qingquan; Zhao, Yanmin; Zeng, Wenhua; Yi, Qingfeng; Cao, Dafu

doi:10.1080/03602559.2012.718400

Cited by 51 publications

(16 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hyaluronan is the only non-sulfated glycosaminoglycan and plays an important role in lubrication, water sorption and water retention [114] , [115] . In bone, hyaluronan has the effect of recruiting osteoblast precursor cells from the bone marrow as well as regulating bone remodeling by controlling behaviors of osteoclast, osteoblast and osteocyte and being involved in the osteogenic differentiation of MSCs [116] , [117] . Collagen/GAGs scaffolds have been widely investigated for various tissue engineering such as skin, peripheral nerve, tendon, and cartilage [118] , [119] , [120] , [121] .…”

Section: Collagen Based Composite Scaffolds For Bone Regenerationmentioning

confidence: 99%

The development of collagen based composite scaffolds for bone regeneration

Zhang

Chen

et al. 2018

Bioactive Materials

346

215

View full text Add to dashboard Cite

Bone is consisted of bone matrix, cells and bioactive factors, and bone matrix is the combination of inorganic minerals and organic polymers. Type I collagen fibril made of five triple-helical collagen chains is the main organic polymer in bone matrix. It plays an important role in the bone formation and remodeling process. Moreover, collagen is one of the most commonly used scaffold materials for bone tissue engineering due to its excellent biocompatibility and biodegradability. However, the low mechanical strength and osteoinductivity of collagen limit its wider applications in bone regeneration field. By incorporating different biomaterials, the properties such as porosity, structural stability, osteoinductivity, osteogenicity of collagen matrixes can be largely improved. This review summarizes and categorizes different kinds of biomaterials including bioceramic, carbon and polymer materials used as components to fabricate collagen based composite scaffolds for bone regeneration. Moreover, the possible directions of future research and development in this field are also proposed.

show abstract

Section: Collagen Based Composite Scaffolds For Bone Regenerationmentioning

confidence: 99%

The development of collagen based composite scaffolds for bone regeneration

Zhang

Chen

et al. 2018

Bioactive Materials

346

215

View full text Add to dashboard Cite

show abstract

“…On the other hand, the WUC of SFs was much higher with the value of 405% as opposed to 350 and 232% for SF/gelatin nanofiber mats at the blend ratios of 90/10 and 70/30, respectively, as illustrated in Figure 7. Such a finding was ascribed to the incorporation of gelatin into SF matrices with GTA crosslinking effect, resulting in denser structures and increasing the compaction degree among molecules so that it was difficult for the entry of water molecules (Zhou et al, 2013). Besides, higher gelatin contents led to lower fiber porosity, thereby limiting the water diffusion into fiber mats.…”

Section: Contact Angle and Water Uptake Capacitymentioning

confidence: 99%

Fabrication and Characterization of Electrospun Silk Fibroin/Gelatin Scaffolds Crosslinked With Glutaraldehyde Vapor

Mohammadzadehmoghadam

Dong

2019

Front. Mater.

View full text Add to dashboard Cite

Bombyx mori silk fibroin (SF) /gelatin nanofiber mats with different blend ratios of 100/0, 90/10, and 70/30 were prepared by electrospinning and crosslinked with glutaraldehyde (GTA) vapor at room temperature. GTA was shown to induce the conformational transition of SFs from random coils to β sheets along with increasing nanofiber diameters with the addition of gelatin into SFs. It was found that by increasing the gelatin content, crosslinking degree was enhanced from 34% for pure SF nanofiber mats to 43% for SF/gelatin counterparts at the blend ratio of 70/30, which directly affected mechanical properties, porosity, and water uptake capacity (WUC) of prepared nanofiber mats. The addition of 10 and 30 wt% gelatin into SFs improved tensile strengths of SF/gelatin nanofiber mats by 10 and 27% along with moderate increases in Young's modulus by 12 and 27%, respectively, as opposed to plain SF counterparts. However, both porosity and WUC were found to decrease from 62 to 405% for pristine SF nanofiber mats to 47 and 232% for SF/gelatin counterparts at the blend ratio of 70/30 accordingly. To further evaluate the combined effect of GTA crosslinking and gelatin content on biological response of SF/gelatin scaffolds, the proliferation assay using 3T3 mouse fibroblast was conducted. In comparison with pure SFs, cell proliferation rate was lower for SF/gelatin constructs, which declined when the gelatin content increased. These results indicated that the adverse effect of GTA crosslinking on cell response may be ascribed to imposed changes in morphology and physiochemical properties of SF/gelatin nanofiber mats. Although crosslinking could be used to improve mechanical properties of nanofiber mats, it reduced their capacity to support the cell activity. GTA optimization is required to further modulate the physico-chemical properties of SF/gelatin nanofiber mats in order to obtain stable materials with favorable bioactive properties and promote cellular responses for tissue engineering applications.

show abstract

“…Since the mechanical properties of pure hyaluronic acid scaffolds cannot always satisfy the clinical requirements, natural and synthetic polymers have been mixed with hyaluronic acid to produce composite scaffolds. For example, gelatin/hyaluronic acid composite scaffolds were fabricated using crosslinking [ 18 , 19 , 20 , 21 ] and electrospinning [ 22 ] strategies. Furthermore, hyaluronic acid was combined with polycaprolactone (PCL), a synthetic polymer commonly used for tissue engineering, to improve the mechanical properties of composite scaffolds.…”

Section: Introductionmentioning

confidence: 99%

Polycaprolactone/Gelatin/Hyaluronic Acid Electrospun Scaffolds to Mimic Glioblastoma Extracellular Matrix

et al. 2020

View full text Add to dashboard Cite

Glioblastoma (GBM), one of the most malignant types of human brain tumor, is resistant to conventional treatments and is associated with poor survival. Since the 3D extracellular matrix (ECM) of GBM microenvironment plays a significant role on the tumor behavior, the engineering of the ECM will help us to get more information on the tumor behavior and to define novel therapeutic strategies. In this study, polycaprolactone (PCL)/gelatin(Gel)/hyaluronic acid(HA) composite scaffolds with aligned and randomly oriented nanofibers were successfully fabricated by electrospinning for mimicking the extracellular matrix of GBM tumor. We investigated the effect of nanotopography and components of fibers on the mechanical, morphological, and hydrophilic properties of electrospun nanofiber as well as their biocompatibility properties. Fourier transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC) have been used to investigate possible interactions between components. The mean fiber diameter in the nanofiber matrix was increased with the presence of HA at low collector rotation speed. Moreover, the rotational velocity of the collector affected the fiber diameters as well as their homogenous distribution. Water contact angle measurements confirmed that hyaluronic acid-incorporated aligned nanofibers were more hydrophilic than that of random nanofibers. In addition, PCL/Gel/HA nanofibrous scaffold (7.9 MPa) exhibited a significant decrease in tensile strength compared to PCL/Gel nanofibrous mat (19.2 MPa). In-vitro biocompatibilities of nanofiber scaffolds were tested with glioblastoma cells (U251), and the PCL/Gel/HA scaffolds with random nanofiber showed improved cell adhesion and proliferation. On the other hand, PCL/Gel/HA scaffolds with aligned nanofiber were found suitable for enhancing axon growth and elongation supporting intracellular communication. Based on these results, PCL/Gel/HA composite scaffolds are excellent candidates as a biomimetic matrix for GBM and the study of the tumor.

show abstract

Effect of Chemical Cross-linking on Properties of Gelatin/Hyaluronic Acid Composite Hydrogels

Cited by 51 publications

References 20 publications

The development of collagen based composite scaffolds for bone regeneration

The development of collagen based composite scaffolds for bone regeneration

Fabrication and Characterization of Electrospun Silk Fibroin/Gelatin Scaffolds Crosslinked With Glutaraldehyde Vapor

Polycaprolactone/Gelatin/Hyaluronic Acid Electrospun Scaffolds to Mimic Glioblastoma Extracellular Matrix

Contact Info

Product

Resources

About