Effect of changes in nitrogen nutrition on the polyamine content of Alnus glutinosa

Abstract

Abstract. The principal polyamines in Alnus glutinosa roots, nodules and root pressure sap, putrescine, spermidine and spermine, were quantified by reversed‐phase, high‐performance liquid chromatography with fluorescence detection following precolumn derivatization with 9‐fluorenylmethyl chloroformate and 1‐ada‐mantanamine. Putrescine was the major component of all tissues and sap. It comprised 70% or more of the polyamine pool except in roots of KNO3‐fed plants, in which similar amounts of putrescine and spermidine were present at levels five‐fold lower than plants fed (NH4)2SO4. Polyamine levels in nodules were 50% greater than in roots. The polyamine content of roots and nodules was not altered significantly when the nitrogen nutrition was changed from sole reliance on nitrogen fixation to partial or complete utilization of (NH4)2SO4. However, the polyamine content of root pressure sap from nodulated plants increased almost four‐fold when they were fed with increasing concentrations of NH4NO3, although the total polyamine content remained low (5mmol m−3 sap). The polyamine content of the Alnus root system changed with plant age. In particular, the spermidine content of both roots and nodules was higher in 10‐ as compared to 16‐week‐old plants.

Polyamines, indole-3-acetic acid and abscisic acid in rice phloem sap

“…Derivatization of polyamines were carried out after Tonin et al . [19] . The supernatant (200 pl) was mixed with 25 Ml 5% perchloric acid neutralized with saturated Na2CO3 and 25 pl 1 M borate buffer adjusted to pH 6 .3 with NaOH .…”

Stimulation of growth and nitrate assimilation inLeucaena leucocephala seedlings in response to spermidine supply

“…The effect of exogenous polyamine supply on nitrogen assimilation, however has not been examined, although the process is of fundamental importance to plant growth and development. Moreover, polyamine effect on nitrogen assimilation may be expected because of the observation that (a) nitrogen influences the endogenous polyamine content (Smith 1985, Tonin et al 1991 and (b) polyamines can act as source of nitrogen, at least in cultured tissues (Bagni et al 1978, Balint et al 1987. The effect of Spd on growth and nitrate assimilation in cotyledons, roots and leaves of the seedlings of a tree species, Leucaena ieucocephala was studied in the present investigation, to examine this possibility.…”