Effect of Chain Length of Alcohol on the Lipase-Catalyzed Esterification of Propionic Acid in Supercritical Carbon Dioxide

Abstract

The esterification of propionic acid was investigated using three different alcohols, namely, isopropyl alcohol, isobutyl alcohol, and isoamyl alcohol. The variation of conversion with time for the synthesis of isoamyl propionate was investigated in the presence of five enzymes. Novozym 435 showed the highest activity, and this was used as the enzyme for investigating the various parameters that influence the esterification reaction. The Ping-Pong Bi-Bi model with inhibition by both acid and alcohol was used to model the experimental data and determine the kinetics of the esterification reaction.

Study of the Influence of Microwave and Conventional Heating on the Lipase-Catalyzed Esterification of Lauric Acid with Different Alcohols

“…It is suggested that the use of microwaves as a heat source to change the interactions between the enzyme and its microenvironment prevent the thermal denaturation of the enzyme. Also, the support provides stability to the enzyme so that it does not degrade at high temperatures [19]. The effect of microwave radiation on enzyme activity is a function of enzyme hydration [20].…”

Activity of immobilized lipase from Candida antarctica (Lipozyme 435) and its performance on the esterification of oleic acid in supercritical carbon dioxide

“…In spite of the decreased residual activity of the immobilized lipase after the treatment with supercritical CO 2 , the enzyme can still catalyze reactions in supercritical media efficiently, as demonstrated by several studies [40,[51][52][53][54][55]. Therefore, the study of the esterification reaction of oleic acid ( Figure 4) using immobilized Lipozyme 435 as a catalyst was carried out in supercritical CO 2 .…”

Recent Advances in Engineering of Media for Enzymatic Catalysis with Lipase

“…As a green medium, supercritical carbon dioxide (scCO2) is one of most suitable solvents for lipase catalysis used to produce flavors esters in the food industry. The lipase-biocatalyzed (Novozym 435) esterification of propionic acid with isopropyl alcohol, isobutyl alcohol and isoamyl alcohol in supercritical carbon dioxide 48 has been reported. The initial reaction rate decreases when the chain length of the primary alcohol increases.…”