Effect of cetyltrimethylammonium chloride on electroosmotic and electrophoretic mobilities in capillary zone electrophoresis

Kaneta, Takashi; Tanaka, Shunitz; Taga, Mitsuhiko

doi:10.1016/0021-9673(93)83189-y

Cited by 43 publications

(12 citation statements)

References 18 publications

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“…By reversing the EOF to match the direction of the anion migration, the analysis time was decreased while high peak efficiencies were obtained. Cationic surfactants such as cetyltrimethylammonium or tetradecyltrimethylammonium salts have been widely used to reverse EOF to operate in a co-EOF migration [22,23]. We selected CTAC as our EOF modifier.…”

Section: Selection Of Eof Modifier Electrolyte and Phmentioning

confidence: 99%

Determination of nitrate and nitrite in rat brain perfusates by capillary electrophoresis

et al. 2004

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A fast and simple method for the direct, simultaneous detection of nitrite (NO(2) (-)) and nitrate (NO(3) (-)) in rat striatum has been developed using a capillary electrophoresis separation of low-flow push-pull perfusion samples. The method was optimized primarily for nitrite because nitrite is more important physiologically and is found at lower levels than nitrate. We obtained a complete separation of NO(2) (-) and NO(3) (-) in rat striatum within 1.5 min. Optimal CE separations were achieved with 20 mM phosphate, 2 mM cetyltrimethylammonium chloride (CTAC) buffer at pH 3.5. The samples were injected electrokinetically for 2 s into a 40 cm x 75 microm ID fused-silica capillary. The separation voltage was 10 kV (negative polarity), and the injection voltage was 16 kV (negative polarity). UV detection was performed at 214 nm. The limits of detection obtained at a signal-to-noise ratio (S/N) of 3 for nitrite and nitrate were 0.96 and 2.86 microM. This is one of the fastest separations of nitrite and nitrate of a biological sample ever reported. Interference produced by the high physiological level of chloride is successfully minimized by use of CTAC in the run buffer.

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Section: Selection Of Eof Modifier Electrolyte and Phmentioning

confidence: 99%

Determination of nitrate and nitrite in rat brain perfusates by capillary electrophoresis

et al. 2004

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“…[ll, 12, 15-18]. Reversal of the direction of the electroosmotic flow is indicative of specific adsorption of counter ions in the interfacial region between the capillary wall and the electrolyte solution [19]; this affects the magnitude and sign of the zeta potential and may mask the silanolic interaction sites for proteins.In the present report, we propose the use of N,N,N,Ntetramethyl-1,3-butanediamine (TMBD) as an effective running electrolyte additive for the efficient separation of basic proteins in bare fused-silica capillaries at different pH values, ranging from 4.0 to 6.5. This pH range may, for example, be suitable for analyzing therapeutic proteins under conditions where their stability and integrity are maintained, and therefore appropriate for detecting low-level degradation products in pharmaceutical preparations.…”

mentioning

confidence: 99%

N,N,N′,N′‐Tetramethyl‐1,3‐butanediamine as effective running electrolyte additive for efficient electrophoretic separation of basic proteins in bare fused‐silica capillaries

Corradini

Cannarsa

1995

Electrophoresis

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The effect of N,N,N',N'-tetramethyl-1,3-butanediamine (TMBD) in the running electrolyte on the electroosmotic flow and the migration behavior of four standard basic proteins in bare fused-silica capillaries was examined at pH 4.0, 5.5, and 6.5. Depending on the electrolyte pH and additive concentration the electroosmotic flow was either cathodic or anodic. A similar Langmuirian-type dependence of the electroosmotic flow on the concentration of TMBD in the running electrolyte was found at the three experimented pH values, which may be indicative of the specific adsorption of the additive in the immobilized region of the electric double layer at the interface between the capillary wall and the electrolyte solution. Electrophoretic separations of the four standard basic proteins performed at the three above pH values, showed well-resolved, efficient and symmetric peaks, demonstrating the utility of this additive for protein electrophoresis in bare fused-silica capillaries. The variations in separation efficiency, peak capacity, resolution and reproducibility of migration times as a function of the additive concentration at pH 6.5 were also examined.

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“…On the contrary, this phenomenon was not observed by Kaneta et al [19] in the investigation of the effect of cetyltrimethylammonium chloride (CTAB) on the electroosmotic mobility in 4 mM phosphate buffer at pH 2.7 or in 10 mM phosphate-20 mM Tris-citrate buffer at pH 5.0 and pH 7.0. The concentration at which the reversed EOF became constant was much smaller than the CMC of CTAC [19]. (Actually, it is less than one tenth of the CMC.)…”

Section: Applicability Of Electroosmotic Mobility As a Parametermentioning

confidence: 49%

Determination of the Critical Micelle Concentration of Cationic Surfactants by Capillary Electrophoresis

Lin

Wang

Chiu

et al. 1999

J. High Resol. Chromatogr.

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Effect of cetyltrimethylammonium chloride on electroosmotic and electrophoretic mobilities in capillary zone electrophoresis

Cited by 43 publications

References 18 publications

Determination of nitrate and nitrite in rat brain perfusates by capillary electrophoresis

Determination of nitrate and nitrite in rat brain perfusates by capillary electrophoresis

N,N,N′,N′‐Tetramethyl‐1,3‐butanediamine as effective running electrolyte additive for efficient electrophoretic separation of basic proteins in bare fused‐silica capillaries

Determination of the Critical Micelle Concentration of Cationic Surfactants by Capillary Electrophoresis

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