Effect of cervical and thoracic vagal stimulation on luminal serotonin release and regional blood flow in cats

Zinner, Michael J.; Jaffe, Bernard M.; deMagistris, L.; Dahlström, Annica; Ahlman, Håkan

doi:10.1016/0016-5085(82)90076-2

Cited by 36 publications

(6 citation statements)

References 16 publications

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“…Endoluminal application of 5HT caused the hyperemia of cat intestinal segment and this response was prevented by local anesthesia applied to the luminal surface (Grönstad et al 1986;Zinner et al 1982). The functional significance, however, of luminally released 5HT from EC cells is not understood completely.…”

Section: Fig 5a-c A′-b′mentioning

confidence: 92%

“…However, a number of in vivo studies have shown a measurable amount of serotonin (5HT) in the intestinal lumen of various species. Known stimulants for such luminal release of 5HT include vagal nerve stimulation (Ahlman et al 1981;Grönstad et al 1987;Zinner et al 1982), luminal acidification (Kellum et al 1983;Resnick and Gray 1962), food intake (Ferrara et al 1987) or an increase in luminal pressure (Bülbring and Crema 1959). Luminal release of 5HT has also been examined in vitro using isolated mucosa/submucosal sheets, and known stimulants include mucosal acidification and cholinergic and β-adrenergic mechanisms Miller 1982, 1983;Kellum et al 1983Kellum et al , 1984a.…”

Section: Introductionmentioning

confidence: 99%

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Immunoelectron microscopic study of the luminal release of serotonin from rat enterochromaffin cells induced by high intraluminal pressure

Fujimiya

Okumiya²,

Kuwahara

1997

Histochemistry and Cell Biology

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Since definitive morphological studies showing the luminal release of serotonin have not been reported, we used a perfused system which allows physiological monitoring and biochemical as well as morphological evidence indicating release of serotonin from enterochromaffin cells. Isolated vascularly and luminally perfused rat duodenums exposed to 5-35 cmH2O of luminal pressure were measured for release of serotonin into the blood vessels and intestinal lumen. Immediately after raising the luminal pressure, the duodenum was fixed for immunoelectron microscopic localization of serotonin. Peristaltic contraction and serotonin content of the perfusates were continuously measured. The luminal release of serotonin increased with elevated intraluminal pressure, but the vascular release of serotonin was not altered. Tetrodotoxin had no effect on the pressure-stimulated luminal serotonin release. Enterochromaffin cells in control animals without increased luminal pressure contained immunogold-labeled secretory granules in the apical and basal cytoplasm. After intraluminal pressure increased, many apical secretory granules were no longer dense and immunogold particles were localized over the cytoplasmic matrix and microvilli. These findings indicate that luminal serotonin release is increased after raising the intraluminal pressure and serotonin, normally stored in the secretory granules of enterochromaffin cells, appears to be released into the cytoplasmic matrix and then diffuses or is transported into the intestinal lumen.

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Section: Fig 5a-c A′-b′mentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Immunoelectron microscopic study of the luminal release of serotonin from rat enterochromaffin cells induced by high intraluminal pressure

Fujimiya

Okumiya²,

Kuwahara

1997

Histochemistry and Cell Biology

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“…In fact, it has been shown that the number of serotonin-containing enetrochromaffin (EC) cells changed in the ulcerative colitis patients (Friedmann 1970;Ahonen et al 1976;Kyösola et al 1977;Capurso et al 1997). Serotonin regulates peristaltic movement (Bülbring and Lin 1958;Gershon and Wade 1993;Gershon et al 1994), water and electrolyte secretion (Cook and Reddix 1994), as well as mucosal blood flow (Zinner et al 1982;Grönstad et al 1986) in the normal intestine. The administration of serotonin caused diarrhea in 85-100% in fasted mice (Kadowaki et al 1996).…”

Section: Introductionmentioning

confidence: 99%

Changes in number of serotonin-containing cells and serotonin levels in the intestinal mucosa of rats with colitis induced by dextran sodium sulfate

Oshima

Fujimura

Fukimiya³

1999

Histochemistry and Cell Biology

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The role of serotonin in the pathogenesis of inflammatory bowel disease has not been fully studied. We examined the changes in the serotonin level and the density of serotonin-containing enterochromaffin (EC) and mast cells in the intestinal mucosa of dextran sodium sulfate (DSS)-induced colitis in rats. Rats were treated with 1.5% DSS for 1 month. Serotonin levels were biochemically measured and the density of epithelial EC cells and mucosal mast cells was quantified by serotonin immunohistochemistry. DSS caused malnutrition due to chronic diarrhea. Infiltrated inflammatory cells were microscopically observed in the colonic wall with intact epithelium. The serotonin content in the mucosa/submucosa tissue was increased in the proximal and distal colon in DSS-treated rats, compared to that in control rats. The density of EC cells in the epithelium also increased in the proximal and distal colon in DSS-treated rats. In contrast, the density of mast cells in the lamina propria dramatically increased in the distal, but not in the proximal colon in DSS-treated rats. This discrepancy implies the serotonin released from EC cells and from mast cells may play different roles in the pathogenesis of DSS-induced colitis.

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“…In contrast, others showed that 5-HT is also released into the intestinal lumen (44,45,46,47). Electrical stimulation of the vagus nerves or duodenal acidification evokes 5-HT release from EC cells into the intestinal lumen in concentrations as high as 2 µM (46, 48, 49). …”

Section: Luminal Release Of 5-htmentioning

confidence: 99%

Interdigestive migrating motor complex -its mechanism and clinical importance

Takahashi

2013

J Smooth Muscle Res

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Migrating motor complex (MMC) is well characterized by the appearance of gastrointestinal (GI) contractions in the interdigestive state. The physiological importance of gastric MMC is a mechanical and chemical cleansing of the empty stomach in preparation for the next meal. MMC cycle is mediated via the interaction between motilin and 5-hydroxytryptamine (5-HT) by the positive feedback mechanism in conscious dogs. Luminal administration of 5-HT initiates duodenal phase II and phase III with a concomitant increase of plasma motilin release. Duodenal 5-HT concentration is increased during gastric phase II and phase III. Intravenous infusion of motilin increases luminal 5-HT content and induces phase III. 5-HT4 antagonists significantly inhibit both of gastric and intestinal phase III, while 5-HT3 antagonists inhibit only gastric phase III. These suggest that gastric MMC is regulated via vagus, 5-HT3/4 receptors and motilin, while intestinal MMC is regulated via intrinsic primary afferent neurons (IPAN) and 5-HT4 receptors. We propose the possibility that maximally released motilin by a positive feedback depletes 5-HT granules in the duodenal EC cells, resulting in no more contractions. Stress is highly associated with the pathogenesis of functional dyspepsia (FD). Acoustic stress attenuates gastric phase III without affecting intestinal phase III in conscious dogs, via reduced vagal activity. Subset of FD patients shows reduced vagal activity and impaired gastric phase III. The impaired gastric MMC may aggravate dyspeptic symptoms following a food ingestion. Maintaining MMC cycle in the interdigestive state is an important factor to prevent the postprandial dyspeptic symptoms.

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Effect of cervical and thoracic vagal stimulation on luminal serotonin release and regional blood flow in cats

Cited by 36 publications

References 16 publications

Immunoelectron microscopic study of the luminal release of serotonin from rat enterochromaffin cells induced by high intraluminal pressure

Immunoelectron microscopic study of the luminal release of serotonin from rat enterochromaffin cells induced by high intraluminal pressure

Changes in number of serotonin-containing cells and serotonin levels in the intestinal mucosa of rats with colitis induced by dextran sodium sulfate

Interdigestive migrating motor complex -its mechanism and clinical importance

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