Effect of cerium oxide on erythrocyte deformability in rat lower extremity ischemia reperfusion injury

Tatar, Tolga; Polat, Yücel; Çomu, Faruk Metin; Kartal, Hakan; Arslan, M; Küçük, Ayşegül

doi:10.4149/bll_2018_080

Cited by 9 publications

(11 citation statements)

References 14 publications

(17 reference statements)

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“…Cerium oxide (Sigma Aldrich ® , Cerium oxide, aqueous nanoparticle dispersion, 100 mL) was given (0.5 mg.kg −1, i.p) 30 minutes before the ischemia period. 16,19,22,23 Ischemia reperfusion-sevoflurane (IRS): Rats were only subjected to midline laparotomy. An atraumatic microvascular clamp was placed on the infrarenal abdominal aorta for 120 minutes then the clamp was removed and reperfused for 120 minutes.…”

Section: Animals and Experimental Protocolmentioning

confidence: 99%

“…11,12 Nonetheless, there are few studies about in vitro and in vivo inflammatory effects associated with cerium oxide, especially how it might alter systemic inflammatory responses. 13 Also numerous studies have indicated the potential use of cerium oxide nanoparticles on cardiomyopathy, 12 stroke, 14 ovarian cancer, 15 sepsis, 16 obesity, 17 hepatic IR, 18 lower extremity IR, 19 and intestinal IR. 20 However, in these studies the effect of cerium oxide was investigated alone.…”

Section: Introductionmentioning

confidence: 99%

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<p>The Effect of Cerium Oxide on Lung Tissue in Lower Extremity Ischemia Reperfusion Injury in Sevoflurane Administered Rats</p>

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et al. 2020

IJN

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Introduction: We aimed to investigate the effects of cerium oxide, applied before the sevoflurane anesthesia, on lung tissue in rats with lower extremity ischemia-reperfusion (IR). Materials and Methods: A total of 30 rats were randomly divided into five groups as; control (C), IR, cerium oxide-IR (CO-IR), IR-sevoflurane (IRS), and cerium oxide-IRsevoflurane (CO-IRS). In the CO-IR group, 30 minutes after the injection of cerium oxide (0.5 mg/kg, intraperitoneal (i.p)), an atraumatic microvascular clamp was placed on the infrarenal abdominal aorta for 120 minutes. Then, the clamp was removed and reperfused for 120 minutes. Sevoflurane was applied in 100% oxygen at a rate of 2.3% at 4 L/min during IR. The blood samples were taken for biochemical analysis and the lung tissue samples were taken for histological analysis. Results: Neutrophil infiltration/aggregation was significantly higher in the IR group than in the C and CO-IRS groups. The alveolar wall thickness and total lung injury scores were significantly higher in the IR group than in the C, IRS, CO-IR and CO-IRS groups. Discussion: We determined that the administration of 0.5 mg/kg dose of cerium oxide with sevoflurane reduces the oxidative stress and corrects IR-related damage in lung tissue. Our results show that the administration of cerium oxide before IR and the administration of sevoflurane during IR have a protective effect in rats.

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Section: Animals and Experimental Protocolmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

<p>The Effect of Cerium Oxide on Lung Tissue in Lower Extremity Ischemia Reperfusion Injury in Sevoflurane Administered Rats</p>

Tunçay

Şıvgın

Özdemirkan

et al. 2020

IJN

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“…In inflamed joints, the HA concentration decreases by depolymerization, the intraarticular application of high molecular weight HA might be an essential therapeutic regimen to restore the natural barrier against PMN migration and to interrupt the inflammatory cascade [ 36 ]. Cerium oxide nanoparticles, widely applied in our life [ 37 ], have recently come into consideration for biomedical use due to their potent antioxidant properties and have been proposed as a treatment for oxidative stress-associated chronic diseases [ 38 , 39 , 40 ]. Cerium oxide nanoparticles present the mimetic activity of superoxide dismutase.…”

Section: Discussionmentioning

confidence: 99%

Hyaluronic Acid Loaded with Cerium Oxide Nanoparticles as Antioxidant in Hydrogen Peroxide Induced Chondrocytes Injury: An In Vitro Osteoarthritis Model

et al. 2020

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Osteoarthritis (OA) is the most common joint disease type and is accompanied by varying degrees of functional limitation. Both hyaluronic acid (HA) joint injections and pain relievers are efficient treatments for early-stage osteoarthritis. However, for the decomposition by hyaluronidase and free radicals in the knee joint, HA injection treatment has limited effect time. The cerium oxide nanoparticles (CeO2) is a long time free radical scavenger. CeO2 combined with HA expected, may extend the HA decomposition time and have a positive effect on osteoarthritis therapy. In this study, CeO2 was successfully synthesized using the hydrothermal method with a particle size of about 120 nm, which possessed excellent dispersibility in the culture medium. The in vitro OA model was established by cell treated with H2O2 for 30 min. Our study found that the inhibition of chondrocyte proliferation dose-dependently increased with H2O2 concentration but was significantly decreased by supplementation of cerium oxide nanoparticles. COL2a1 and ACAN gene expression in chondrocytes was significantly decreased after H2O2 treatment; however, the tendency was changed after cerium oxide nanoparticles treatment, which suggested that damaged chondrocytes were protected against oxidative stress. These findings suggest that cerium oxide nanoparticles are potential therapeutic applications in the early stage of OA.

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“…e establishment of lower-extremity I/R injury model on rats was conducted according to the instruction described previously [14]. Forty-eight Sprague Dawley (SD) rats were obtained from Kay Biological Technology Co., Ltd. (Shanghai, China) and adapted for 7 days after arrival.…”

Section: Animals and The Establishment Of Lower-extremity I/r Injury mentioning

confidence: 99%

Resveratrol Alleviates Vascular Endothelial Damage Caused by Lower-Extremity Ischemia Reperfusion (I/R) through Regulating Keap1/Nrf2 Signaling-Mediated Oxidative Stress

Song

Liu

Zeng

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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The present study aims to investigate the protective effects of Resveratrol (RSV) against vascular endothelial damage caused by lower-extremity I/R and the underlying preliminary mechanism. The in vitro hypoxia reoxygenation (HR) model was established on HUVECs. Lower-extremity I/R model was established on rats followed by being treated with RSV and the pathological state of artery was evaluated by HE and EVG staining, while the apoptotic state of artery was detected by TUNEL assay. The cell viability was detected by MTT assay and the apoptotic state of cells was determined by Hoechst test and flow cytometry assay. DCFH-DA staining was used to measure the level of ROS and the production of MDA and SOD was measured by commercial kits. The expression level of Nrf2, Keap1, HO-1, Bcl-2, Bax, and Caspase-3 in cells was determined by Western blot. Nrf2 was knocked down by siRNA technology. Overall, our data indicated that increased cell viability, declined apoptotic rate, and alleviated oxidative stress were observed in RSV treated HR HUVECs, which were significantly reversed by knocking down Nrf2. Animal experiment revealed that the pathological and apoptotic state of femoral artery were dramatically ameliorated by the treatment of RSV, accompanied by the alleviated oxidative stress, which were abolished by the co-administration of ML385, an inhibitor of Nrf2. Taken together, our data revealed that RSV might alleviate vascular endothelial injury induced by lower-extremity I/R injury through regulating Keap1/Nrf2 signaling-mediated oxidative stress.

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Effect of cerium oxide on erythrocyte deformability in rat lower extremity ischemia reperfusion injury

Cited by 9 publications

References 14 publications

<p>The Effect of Cerium Oxide on Lung Tissue in Lower Extremity Ischemia Reperfusion Injury in Sevoflurane Administered Rats</p>

<p>The Effect of Cerium Oxide on Lung Tissue in Lower Extremity Ischemia Reperfusion Injury in Sevoflurane Administered Rats</p>

Hyaluronic Acid Loaded with Cerium Oxide Nanoparticles as Antioxidant in Hydrogen Peroxide Induced Chondrocytes Injury: An In Vitro Osteoarthritis Model

Resveratrol Alleviates Vascular Endothelial Damage Caused by Lower-Extremity Ischemia Reperfusion (I/R) through Regulating Keap1/Nrf2 Signaling-Mediated Oxidative Stress

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