Effect of cerium ion on the proliferation, differentiation and mineralization function of primary mouse osteoblasts in vitro

Zhang, Jinchao; Liu, Cuilian; LI, Yaping; Sun, Jing; Wang, Peng; Di, Keqian; Zhao, Yanyan

doi:10.1016/s1002-0721(09)60067-3

Cited by 60 publications

(38 citation statements)

References 16 publications

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“…Thus it is likely that Ce intervenes in bone‐remodeling process and affects bone cell function. We previously reported that the effects of Ce on the proliferation, differentiation, and mineralization function of primary OBs depended on the concentration and culture time at cell level [Zhang et al, 2010]. However, the potential effect and the mechanism of Ce on bone metabolism are not well understood.…”

mentioning

confidence: 99%

TGF‐β/BMP signaling pathway is involved in cerium‐promoted osteogenic differentiation of mesenchymal stem cells

Liu

Zhang

et al. 2013

J of Cellular Biochemistry

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The extensive applications of cerium (Ce) increased the chance of human exposure to Ce and its compounds. It was reported that Ce was mainly deposited in the bone after administration. However, the potential effect and mechanism of Ce on bone metabolism are not well understood. In this study, we investigated the cellular effects of Ce on the differentiation of mesenchymal stem cells (MSCs) and the associated molecular mechanisms. The results indicated that Ce promoted the osteogenic differentiation and inhibited the adipogenic differentiation of MSCs at cell level. Genes involved in transforming growth factor-β/bone morphogenetic proteins (TGF-β/BMP) signaling pathway were significantly changed when the MSCs were exposed to 0.0001 µM Ce by RT(2) Profiler™ PCR Array analysis. The expression of genes and proteins related to pathways, osteogenic, and adipogenic biomarkers of MSCs upon interaction with Ce was further confirmed by quantitative real-time reverse transcriptase polymerase chain reaction (Q-PCR) and Western blot analysis. The results suggest that Ce exerts the effects by interacting with bone morphogenetic protein receptor (BMPR) and activates TGF-β/BMP signaling pathway, leads to the up-regulation of the osteogenic master transcription factor, runt-related transcription factor 2 (Runx 2), and the down-regulation of the adipocytic master transcription factor, peroxisome proliferator-activated receptor gamma 2 (PPARγ2). Runx2, which subsequently up-regulates osteoblast (OB) marker genes collagen I (Col I) and BMP2 at early stages, alkaline phosphatase (ALP), and osteocalcin (OCN) at later stages of differentiation, thus driving MSCs to differentiate into OBs. The results provide novel evidence to elucidate the mechanisms of bone metabolism by Ce.

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mentioning

confidence: 99%

TGF‐β/BMP signaling pathway is involved in cerium‐promoted osteogenic differentiation of mesenchymal stem cells

Liu

Zhang

et al. 2013

J of Cellular Biochemistry

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“…[74] In fact, our results showed a weak but significant increase in mitochondrial activity (24-h MTT assay) for 1000 and 1200 µM Gd (Fig. 2).…”

Section: Threshold Values Of Cyto-genototicity Values Of Individual Ionsmentioning

confidence: 64%

“…Accordingly, it has been reported that some lanthanides induce mitochondrial dysfunction that could be the cause of this cytotoxic effect. [74,[76][77] It should be noticed that Gd did not show any toxic effect at these concentrations. Likewise, for 1400 µM an important difference between La vs Gd results was also observed in viability with Acridine Orange dye.…”

Section: Threshold Values Of Cyto-genototicity Values Of Individual Ionsmentioning

confidence: 79%

Cellular response to rare earth mixtures (La and Gd) as components of degradable Mg alloys for medical applications

Grillo

Álvarez

Mele

2014

Colloids and Surfaces B: Biointerfaces

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“…13 Lanthanide can potentially replace calcium element in bone due to similar ionic radii and coordination number. [13][14][15] Lanthanide affect bone remodeling cycle having proliferative effect on osteoblast 16 and inhibitory effect on osteoclasts 15 which leads to high affinity for bone. 17 Eu is considered as important rare earth element because of its 4f-4f intraorbital electronic transitions which can span both visible and near-infrared (NIR) range.…”

Section: Introductionmentioning

confidence: 99%

Elucidating the role of size of hydroxyl apatite particles toward the development of competent antiosteoporotic bioceramic materials: In vitro and in vivo studies

Kaur

Singh

Anand

et al. 2019

J Biomedical Materials Res

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Osteoporosis caused by overdose of steroids is one of the major concerns for the orthopedic surgeons. Current therapeutic strategies offer limited success due to their inability to regenerate damaged bone at osteoporosis site. Therefore, there is an urgent need to develop a material having bone regeneration ability and also, ability to cure osteoporosis simultaneously. In this work, nanosized and microsized hydroxyl apatite (HAp) particles doped with europium (Eu) were prepared for diagnostic and therapeutic applications in biomedical engineering. Particles were characterized by X‐ray diffraction to confirm the formation of HAp phase and transmission electron microscopy technique has been used to explore the size of microparticle and nanoparticle. In vitro release of antibiotic drug and degradation behavior in two different pHs of phosphate buffered saline was checked. Controlled drug release behavior and conversion of degraded ions into HAp is estimated by Higuchi's and 3D diffusion model, respectively. Osteoporosis was induced in 36 female Wistar rats by administering dexamethasone once a week for four consecutive weeks. Rats were treated with different doses of nano‐HAp (25, 50, and 100 μg/kg intravenous single dose) and single dose of microsized HAp (100 μg/kg). After treatment, authors have evaluated sensitive biochemical markers of bone in serum. Continuous improvement in ultimate stiffness and Young's modulus of femur shaft of rats was observed with the increase in the dose of nano‐HAp from 25 to 100 μg/kg. Results strongly suggest that europium‐doped nano‐HAp is more effective for treating severe osteoporosis in humans. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1723–1735, 2019.

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Effect of cerium ion on the proliferation, differentiation and mineralization function of primary mouse osteoblasts in vitro

Cited by 60 publications

References 16 publications

TGF‐β/BMP signaling pathway is involved in cerium‐promoted osteogenic differentiation of mesenchymal stem cells

TGF‐β/BMP signaling pathway is involved in cerium‐promoted osteogenic differentiation of mesenchymal stem cells

Cellular response to rare earth mixtures (La and Gd) as components of degradable Mg alloys for medical applications

Elucidating the role of size of hydroxyl apatite particles toward the development of competent antiosteoporotic bioceramic materials: In vitro and in vivo studies

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