Effect of centrifugation and washing on adipose graft viability: A new method to improve graft efficiency

Hoareau, Laurence; Bencharif, Karima; Girard, Anne-Claire; Gence, Lydie; Delarue, Pierre; Hulard, O.; Festy, Franck; Roche, Régis

doi:10.1016/j.bjps.2012.12.033

Cited by 73 publications

(55 citation statements)

References 24 publications

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“…A balance must then be found between interstitial liquid removal and adipose tissue damage. Tissue damage can lead to an increase in the secretion of proinflammatory cytokines, such as Monocyte Chimoattractant Protein 1 (MCP1) or Interleukin 6 (IL6) [63]. Hoareau and Kurita have both shown a decrease in survival with centrifugation forces higher than 4009g for 1 min [63,67].…”

Section: Centrifugationmentioning

confidence: 99%

“…In 2013, Zhu found less oil and greater viability with this system compared to high-speed centrifugation [69]. Some protocols also integrate additional washing steps (e.g., Macrofill by Adip'sculpt; PureGraft by Cytori) that aim to remove the remaining infiltration solution [61,69,70], to get rid of most of the local anesthetic, vasoconstrictors, inflammatory molecules, and death factors (released during liposuction) that may damage the tissue and limit graft success [51,63]. In 2013, only 28 % of surgeons were using a washing step with breast AFG [11].…”

Section: Alternative Processesmentioning

confidence: 99%

“…The remaining infiltration liquid can also be responsible for a higher concentration of pro-inflammatory cytokines secreted by the adipose tissue during aspiration. This may trigger inflammation at the recipient site, with recruitment of immune cells that can eventually lead to increased graft resorption [63].…”

Section: Decantationmentioning

confidence: 99%

“…However, this step requires a centrifuge and probably more time to carry out than simple decantation. Since Coleman described the purification step with 3 min of centrifugation at 3000 rpm (rarely mentioned but corresponding to 900-1200 g, depending upon the rotor used), many studies have investigated the role of centrifugation (speed and time) on the viability and the maintenance of the reinjected tissue [61,63,[65][66][67]. Caution must be taken with this centrifugation step to preserve the integrity of the adipose tissue.…”

Section: Centrifugationmentioning

confidence: 99%

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Autologous Fat Grafting in the Breast: Critical Points and Technique Improvements

et al. 2015

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Section: Centrifugationmentioning

confidence: 99%

Section: Alternative Processesmentioning

confidence: 99%

Section: Decantationmentioning

confidence: 99%

Section: Centrifugationmentioning

confidence: 99%

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Autologous Fat Grafting in the Breast: Critical Points and Technique Improvements

et al. 2015

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“…Washing, therefore, remains as the single most important phase in fat processing. [6] The same authors have proposed a soft centrifugation (400 g/L min [1,000 rpm/L min]) as an alternative following washing of the graft.…”

Section: Introductionmentioning

confidence: 99%

Differential fat harvesting

Farr¹,

Trivisonno²

2014

Plast Aesthet Res

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Aim: Volume replacement with fi llers is regularly performed with the use of diverse volumetric materials to correct different structures around the face, depending on the volume enhancement required and the thickness of the soft tissue envelope. Differential fat harvesting and posterior grafting is performed to place the correct fat parcel size for each target area, expanding the potential applications of fat. Methods: Sixty patients consecutively recruited on a fi rst come basis undergone a facial fat grafting procedure, in private practice setting between March 2012 and October 2013. Fat grafting quantity and quality was predicted for each case. Differential harvesting was performed, with 2 fat parcels size. Processing was performed through washing. Fat infi ltration was carried out through small cannulas or needles depending on the treated area. Outcomes were analysed both by the physicians and the patients at 7 days, 1 month, 3 months and 6 months through a perceived satisfaction questionnaire. Parameters considered were downtime or discomfort, skin benefi ts, volume restoration, reabsorption rate estimated and overall improvement. Results: Full facial differential fat grafting procedure lasted an average of 1.5-2.5 h. Average downtime was 3-4 days. Follow-up was performed to a minimum of 6 months. Both patient and physician overall satisfaction rates were mostly excellent. Adverse events like lumps or irregularities were not encountered. Conclusion: Differential fat harvesting and posterior grafting is a valid alternative, to expand the repertoire of fat use, allow a more homogeneous effect, reduce the potential complications, speed up the process, improve graft survival, and to enhance overall aesthetic outcome.

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Autologous fat transfer—principles, techniques, and outcomes for facial rejuvenation, scars, breast, and buttocks

Roh

Chung

Goyal

et al. 2021

Dermatological Reviews

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Autologous fat transfer is a common method to correct soft-tissue defects. It has been used in various conditions with subcutaneous atrophy induced by trauma or inflammation. Recently, the use has become popular for cosmetic purposes such as facial rejuvenation andbody contouring. In this article, we review the science and method behind its practice and describe other possible indications for autologous fat transfer.

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Effect of centrifugation and washing on adipose graft viability: A new method to improve graft efficiency

Cited by 73 publications

References 24 publications

Autologous Fat Grafting in the Breast: Critical Points and Technique Improvements

Autologous Fat Grafting in the Breast: Critical Points and Technique Improvements

Differential fat harvesting

Autologous fat transfer—principles, techniques, and outcomes for facial rejuvenation, scars, breast, and buttocks

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