Effect of centrifugation and sugar supplementation on the semen cryopreservation of captive collared peccaries (Tayassu tajacu)

Abstract

The present study is aimed at evaluating the effect of centrifugation for seminal plasma removal and the supplementation of fructose or glucose to the Tris-based extender on the kinematic patterns of the motility parameters of frozen-thawed semen obtained from captive collared peccaries (Tayassu tajacu). Semen samples (n = 14) were collected from 10 sexually mature male collared peccaries by electroejaculation. These samples were further evaluated for parameters such as motility, vigor, sperm viability, membrane integrity, and sperm morphology. The samples were divided into four aliquots, and only two of these aliquots were centrifuged. The semen aliquots (centrifuged and raw semen samples) were diluted in Tris-based extenders supplemented with fructose or glucose. Egg yolk (20%) and glycerol (3%) were added to all the samples which were cryopreserved in liquid nitrogen and thawed at 37 °C/1 min. The frozen-thawed semen was evaluated for the same parameters described for the fresh semen. On the other hand, the kinematic motility patterns were evaluated by a computer-aided system. After thawing, it was observed that the values for the total sperm motility were around 30% for all the samples. A negative effect of centrifugation was verified for parameters such as sperm morphology, linearity, straightness, and beat cross frequency (P < 0.05). However, no differences between fructose and glucose were verified for any semen end point (P > 0.05). In conclusion, it is not recommended to centrifuge the ejaculates from collared peccaries prior to conducting the cryopreservative procedures using a Tris-based extender supplemented with fructose or glucose.

Cryopreservation of Aceh Cattle Semen with Date (Phoenix dactylifera) Extract Supplementation

“…Date contains a variety of nutrients such as carbohydrate, protein, antioxidant, and vitamin C that might act as spermatozoa protectants. Sugar contents like sucrose (Rizal et al, 2007), glucose, fructose, and others (Castelo et al, 2010) in extender serve as extracellular cryoprotectants that might protect plasma membrane from damage during cryopreservation (Mukminat et al, 2014;Rizal et al, 2007). Karp (2010) stated that carbohydrate in a cellular environment can be associated with lipid or protein of plasma membrane of cells.…”

Sperm morphological and morphometric evaluation in captive collared peccaries (Pecari tajacu)

“…The stimulatory cycle comprised of 10 stimuli in each voltage, starting from 5-V, followed by voltage increases in steps of 1-V up to 12-V. Each electrical stimulus lasted for 3s with intermittent breaks of 2s. The stimuli were maintained for a duration of 10 min from the beginning of the procedure (Castelo et al 2010). The electroejaculator probe measured 15cm (length) and 1.3cm (diameter); and 12cm were inserted into the rectum of the male.…”

“…Several staining methods such as the brome-phenol blue (Toniolli et al 2010), the eosin-nigrosin (Moraes et al 2010), the diff-quick test (Kim et al 2011), the Cerovský (Fridrichová et al 2011), and the Williams-modified (Am-in et al 2011) are reported for sperm morphology analysis in the swine, that is the domestic species more closely related to the collared peccaries (Cavalcante-Filho et al 1998). In these last ones, only the eosin-nigrosin (Kahwage et al 2010) and the Bengal rose (Castelo et al 2010) staining are described for sperm evaluation, but comparisons among techniques are necessary in order to identify the most suitable for the species.…”

Comparison of Different Glycerol and Egg Yolk Concentrations Added toTris‐based Extender for the Collared Peccaries (Tayassu tajacu) Semen Freezing

“…Recently, Castelo et al. (,b) reported the first success in cryopreserving the collared peccary semen using Tris‐based extenders added of egg yolk (20%) and glycerol (3%). This protocol provided 37% motile sperm after thawing.…”

“…Since that only 3% glycerol was previously tested for collared peccaries semen (Castelo et al. ,b), the search for other concentrations that provide a better cryoprotection is justified to improve the protocol. Based on the fact that glycerol concentrations as higher as 6% are currently used for the semen freezing in domestic animals (Silva et al.…”