Effect of cell media on polymer coated superparamagnetic iron oxide nanoparticles (SPIONs): Colloidal stability, cytotoxicity, and cellular uptake studies

Toxicological Risk Assessment of Emerging Nanomaterials: Cytotoxicity, Cellular Uptake, Effects on Biogenesis and Cell Organelle Activity, Acute Toxicity and Biodistribution of Oxide Nanoparticles

“…In the same way, neutrally charged SPIONs coated with PEG show less cellular interactions on RAW 264.7 cells by TEM and classical microscopy (labeled with Prussian blue) than negatively charged PEGylated-SPIONs [5]. On another side, positively charged PVA-SPIONs seem to have a better internalization by HeLa cells than neutral PVA-SPIONs with an additional influence of the culture medium used, especially depending on the presence of proteins [7]. Thus, the influence of the chemical coating is an important factor, however it seems that the nature of the medium used is much more critical.…”

“…Polymers of different molecular weights (from 2 to 30 kDa) and bearing different chemical groups were used. PEG chains were covalently grafted on the surface of SPIONs (EDC/NHS coupling), while PVA was linked via electrostatic interactions [7].…”

The intensity of internalization and cytotoxicity of superparamagnetic iron oxide nanoparticles with different surface modifications in human tumor and diploid lung cells

“…In serum-free medium, MNPs underwent rapid aggregation apparently because of a high ionic strength of culture medium [30] and no MNPs were found inside the cells. A rapid agglomeration of polyvinyl alcohol/vinyl amine coated magnetite nanoparticles in serum-free medium have reported also Petri-Fink et al [31]. Plasma proteins have been shown to compete for the nanoparticle surface, leading to a protein ''corona'' when particles enter a biological fluid or culture media [32,33].…”

Biomedical nanoparticles modulate specific CD4+T cell stimulation by inhibition of antigen processing in dendritic cells

“…Alteration of immune responses by nanoparticles may represent a form of "nano-immuno-toxic" effect that may seriously undermine future efforts to develop novel therapeutic or diagnostic approaches. Utilizing prototypic fluorochrome-labelled model PVA-SPIONs previously evaluated in several preclinical studies, we herein provide data derived from in vitro studies of DCs treated with such nanoparticles (Petri-Fink et al 2005;Cengelli et al 2006;Hellstern et al 2006;Petri-Fink and Hofmann 2007;von Zur Muhlen et al 2007;Butoescu et al 2008;Petri-Fink et al 2008;Butoescu et al 2009; . Antigen-uptake, antigen-processing, and MHCII expression of MDDCs treated with PVA-SPIONs during 12 h. Endocytotic capacity (antigen uptake) and antigen processing were measured by incubation with a variety of fluorescent conjugates, as previously described (Wikstrom et al 2006;von Garnier et al 2007).…”