Effect of cell imprinting on viability and drug susceptibility of breast cancer cells to doxorubicin

Shahriyari, Fatemeh; Sharifi, Shahriar; Hesar, Milad Eyvazi; Hoshian, Sasha; Taghiabadi, R.; Razaghian, A.; Ghadiri, Majid; Peirovi, Afshin; Nezhad, Amir Sanati; Khademhosseini, Ali

doi:10.1016/j.actbio.2020.06.007

Cited by 14 publications

(10 citation statements)

References 68 publications

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“…In a 3D culture model, stiff ECM induced tumorigenic features in mammary epithelial cells with increased lamina-associated chromatin with more accessible chromatin; a soft ECM in the range of the natural in vivo environment caused in vivo like chromatin profiles (Stowers et al, 2019). Tying in topographic patterning, simply imprinting cell membrane shapes in culture surfaces drastically altered apoptosis and susceptibility to breast cancer drug treatment (Shahriyari et al, 2020). Although 2D cultures have been used a lot for breast cancer work, all of these data from 3D culture setups demonstrate that the culture dimensionality together with the mechanical microenvironment likely affect a variety of cancer cellular processes.…”

Section: D Vs 3d Culturesmentioning

confidence: 99%

The Role of Microenvironmental Cues and Mechanical Loading Milieus in Breast Cancer Cell Progression and Metastasis

Riehl

Kim

Bouzid

et al. 2021

Front. Bioeng. Biotechnol.

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Cancer can disrupt the microenvironments and mechanical homeostatic actions in multiple scales from large tissue modification to altered cellular signaling pathway in mechanotransduction. In this review, we highlight recent progresses in breast cancer cell mechanobiology focusing on cell-microenvironment interaction and mechanical loading regulation of cells. First, the effects of microenvironmental cues on breast cancer cell progression and metastasis will be reviewed with respect to substrate stiffness, chemical/topographic substrate patterning, and 2D vs. 3D cultures. Then, the role of mechanical loading situations such as tensile stretch, compression, and flow-induced shear will be discussed in relation to breast cancer cell mechanobiology and metastasis prevention. Ultimately, the substrate microenvironment and mechanical signal will work together to control cancer cell progression and metastasis. The discussions on breast cancer cell responsiveness to mechanical signals, from static substrate and dynamic loading, and the mechanotransduction pathways involved will facilitate interdisciplinary knowledge transfer, enabling further insights into prognostic markers, mechanically mediated metastasis pathways for therapeutic targets, and model systems required to advance cancer mechanobiology.

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Section: D Vs 3d Culturesmentioning

confidence: 99%

The Role of Microenvironmental Cues and Mechanical Loading Milieus in Breast Cancer Cell Progression and Metastasis

Riehl

Kim

Bouzid

et al. 2021

Front. Bioeng. Biotechnol.

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“…MCF-7 or MDA-MB-231 in rat collagen I were studied [ 73 ]. MDA-MB-231, MDA-MB-468, MCF-7 and SKBR3 cells were printed in GelMA and assessed for sensitivity to cytostatic agents [ 148 , 149 ]. Patient-derived BC cells were bioprinted in PEG hydrogels, functionalized with adhesion peptides and in gelatin-derived hydrogels (GelMA and crosslinked thiolated gelatin).…”

Section: Modelsmentioning

confidence: 99%

The Variety of 3D Breast Cancer Models for the Study of Tumor Physiology and Drug Screening

Frőhlich

2023

IJMS

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Breast cancer is the most common cancer in women and responsible for multiple deaths worldwide. 3D cancer models enable a better representation of tumor physiology than the conventional 2D cultures. This review summarizes the important components of physiologically relevant 3D models and describes the spectrum of 3D breast cancer models, e.g., spheroids, organoids, breast cancer on a chip and bioprinted tissues. The generation of spheroids is relatively standardized and easy to perform. Microfluidic systems allow control over the environment and the inclusion of sensors and can be combined with spheroids or bioprinted models. The strength of bioprinting relies on the spatial control of the cells and the modulation of the extracellular matrix. Except for the predominant use of breast cancer cell lines, the models differ in stromal cell composition, matrices and fluid flow. Organoids are most appropriate for personalized treatment, but all technologies can mimic most aspects of breast cancer physiology. Fetal bovine serum as a culture supplement and Matrigel as a scaffold limit the reproducibility and standardization of the listed 3D models. The integration of adipocytes is needed because they possess an important role in breast cancer.

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“…Arising from denatured collagen, the most abundant BCa ECM protein, gelatin can be functionalized with methacrylate groups to form GelMA, a biomaterial that combines the bioactivity of gelatin with the mechanical tuning provided by the photocrosslinkable methacryloyl functional groups [ 35 ]. GelMA has been used with BCa cell lines (MDA-MB-231, MDA-MB-468, MCF-7, SKBR3 [ 36 , 37 , 38 ]) showing how 3D cultures were more resistant to paclitaxel treatment [ 36 ], or how cell-imprinted GelMA hydrogels (topography-modified) led to enhanced doxorubicin uptake and increased cell death [ 37 ]. While GelMA provides platforms that are mechanically adjustable to match the normal and pathological breast environments, the use of UV crosslinking and photoinitiators may potentially harm primary cells, although this remains unaddressed [ 33 ].…”

Section: Introductionmentioning

confidence: 99%

GelMA, Click-Chemistry Gelatin and Bioprinted Polyethylene Glycol-Based Hydrogels as 3D Ex Vivo Drug Testing Platforms for Patient-Derived Breast Cancer Organoids

et al. 2023

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3D organoid model technologies have led to the development of innovative tools for cancer precision medicine. Yet, the gold standard culture system (Matrigel®) lacks the ability for extensive biophysical manipulation needed to model various cancer microenvironments and has inherent batch-to-batch variability. Tunable hydrogel matrices provide enhanced capability for drug testing in breast cancer (BCa), by better mimicking key physicochemical characteristics of this disease’s extracellular matrix. Here, we encapsulated patient-derived breast cancer cells in bioprinted polyethylene glycol-derived hydrogels (PEG), functionalized with adhesion peptides (RGD, GFOGER and DYIGSR) and gelatin-derived hydrogels (gelatin methacryloyl; GelMA and thiolated-gelatin crosslinked with PEG-4MAL; GelSH). Within ranges of BCa stiffnesses (1–6 kPa), GelMA, GelSH and PEG-based hydrogels successfully supported the growth and organoid formation of HR+,−/HER2+,− primary cancer cells for at least 2–3 weeks, with superior organoid formation within the GelSH biomaterial (up to 268% growth after 15 days). BCa organoids responded to doxorubicin, EP31670 and paclitaxel treatments with increased IC50 concentrations on organoids compared to 2D cultures, and highest IC50 for organoids in GelSH. Cell viability after doxorubicin treatment (1 µM) remained >2-fold higher in the 3D gels compared to 2D and doxorubicin/paclitaxel (both 5 µM) were ~2.75–3-fold less potent in GelSH compared to PEG hydrogels. The data demonstrate the potential of hydrogel matrices as easy-to-use and effective preclinical tools for therapy assessment in patient-derived breast cancer organoids.

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Effect of cell imprinting on viability and drug susceptibility of breast cancer cells to doxorubicin

Cited by 14 publications

References 68 publications

The Role of Microenvironmental Cues and Mechanical Loading Milieus in Breast Cancer Cell Progression and Metastasis

The Role of Microenvironmental Cues and Mechanical Loading Milieus in Breast Cancer Cell Progression and Metastasis

The Variety of 3D Breast Cancer Models for the Study of Tumor Physiology and Drug Screening

GelMA, Click-Chemistry Gelatin and Bioprinted Polyethylene Glycol-Based Hydrogels as 3D Ex Vivo Drug Testing Platforms for Patient-Derived Breast Cancer Organoids

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