Effect of cell-extracellular matrix interaction on myogenic characteristics and artificial skeletal muscle tissue

Ding, Ran; Horie, Masanobu; Nagasaka, Sumire; Ohsumi, Saki; Shimizu, Kazunori; Honda, Hiroyuki; Nagamori, Eiji; Fujita, Hideaki; Kawamoto, Takuo

doi:10.1016/j.jbiosc.2020.02.008

Cited by 5 publications

(5 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, they demonstrated an increased expression of integrin (α 3 β 1 and α 11 β 1 ) by human mesenchymal stem cells (hMSCs) grown on bovine ECM niche, thus, confirming their essential role in mediating cell adhesion and migration [43]. In the contest of muscle development, similar results were obtained by Ding et al that proved the enhanced expression of integrin α 7 β 11, commonly present in myoblasts, on C2C12 cells grown on Matrigel [44]. Thanks to this intensified expression, the interactions between integrin and extracellular proteins, such as tenascin-C and Nephronectin, increased accelerating the myogenic processes [45,46].…”

Section: Discussionsupporting

confidence: 60%

“…Laminin can influence myoblasts differentiation also indirectly by changing cell-cell affinity that represents another crucial step during myogenesis [37,41]. Moreover, previous studies demonstrated that other specific cell proteins involved in cellcell recognition such as N-and M-cadherin appeared to be altered on ECM substrates such as Matrigel [39,44].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Myogenic Potential of Extracellular Matrix Derived from Decellularized Bovine Pericardium

Carton

Francesco

Fusaro³

et al. 2021

IJMS

View full text Add to dashboard Cite

Skeletal muscles represent 40% of body mass and its native regenerative capacity can be permanently lost after a traumatic injury, congenital diseases, or tumor ablation. The absence of physiological regeneration can hinder muscle repair preventing normal muscle tissue functions. To date, tissue engineering (TE) represents one promising option for treating muscle injuries and wasting. In particular, hydrogels derived from the decellularized extracellular matrix (dECM) are widely investigated in tissue engineering applications thanks to their essential role in guiding muscle regeneration. In this work, the myogenic potential of dECM substrate, obtained from decellularized bovine pericardium (Tissuegraft Srl), was evaluated in vitro using C2C12 murine muscle cells. To assess myotubes formation, the width, length, and fusion indexes were measured during the differentiation time course. Additionally, the ability of dECM to support myogenesis was assessed by measuring the expression of specific myogenic markers: α-smooth muscle actin (α-sma), myogenin, and myosin heavy chain (MHC). The results obtained suggest that the dECM niche was able to support and enhance the myogenic potential of C2C12 cells in comparison of those grown on a plastic standard surface. Thus, the use of extracellular matrix proteins, as biomaterial supports, could represent a promising therapeutic strategy for skeletal muscle tissue engineering.

show abstract

Section: Discussionsupporting

confidence: 60%

Section: Discussionmentioning

confidence: 99%

Myogenic Potential of Extracellular Matrix Derived from Decellularized Bovine Pericardium

Carton

Francesco

Fusaro³

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

“…Our results showed that laminin produced the longest myotube and this agreed with Duffy et al , 37 whereas Ding et al observed fibronectin to be superior. 38 Ito et al observed that collagen IV resulted in the longest myotube. 39 There are some conflicting data pertaining to the most myogenic ECM within the context of a singular component.…”

Section: Discussionmentioning

confidence: 99%

Combinatorial extracellular matrix cues with mechanical strain induce differential effects on myogenesis in vitro

et al. 2023

View full text Add to dashboard Cite

show abstract

“…The MuSC surface marker Itga7 has been shown to affect the generation of myogenic progenitor cells and muscle differentiation (Mayer et al, 1997;Rooney et al, 2009;Ding et al, 2020). The expression of Itga7-X1 isoform in QKI-deficient MuSCs and subsequent loss of MuSC polarity provides evidence that maintaining a high Itga7-X2/ Itga7-X1 isoform ratio is a requirement for MuSC polarity.…”

Section: Discussionmentioning

confidence: 99%

Muscle stem cell polarity requires QKI-mediated alternative splicing of Integrin Alpha-7 (Itga7)

Dominici

Richard

2022

Life Sci. Alliance

View full text Add to dashboard Cite

Muscle stem cells (MuSCs) have the ability to carry out the specialized function of cell polarization, which is required for the production of one repopulating cell and one myogenic progenitor cell with muscle regeneration capabilities. The mechanisms which regulate proteins involved in establishing MuSC polarity such as Dmd and Itga7 are currently not well understood. Herein, we define the RNA-binding protein Quaking (QKI) as a major regulator alternative splicing of key MuSC polarity factors including Dmd, Itga7, Mark2, and Numb. We generate a conditional QKI knockout mouse, and for the first time it is shown in vivo that deficiency of QKI in MuSCs results in reduced asymmetric cell divisions, leading to a loss of the myogenic progenitor cell population and striking muscle regeneration defects. Transcriptomic analysis of QKI-deficient MuSCs identifies QKI as a regulator of the splicing events which give rise to the mutually exclusive Itga7-X1 and -X2 isoforms. We observe increased X1 expression in QKI-deficient MuSCs and recapitulate this splicing event using antisense oligonucleotide directed against a quaking binding site within the Itga7 mRNA. Interestingly, recreating this single splicing event is detrimental to the polarization of Itga7 and Dmd proteins, and leads to a drastic reduction of the myogenic progenitor population, highlighting the significance of QKI-mediated alternative splicing of Itga7 in maintaining MuSC polarity. Altogether, these findings define a novel role for QKI as a post-transcriptional regulator of MuSC polarity.

show abstract

Effect of cell-extracellular matrix interaction on myogenic characteristics and artificial skeletal muscle tissue

Cited by 5 publications

References 42 publications

Myogenic Potential of Extracellular Matrix Derived from Decellularized Bovine Pericardium

Myogenic Potential of Extracellular Matrix Derived from Decellularized Bovine Pericardium

Combinatorial extracellular matrix cues with mechanical strain induce differential effects on myogenesis in vitro

Muscle stem cell polarity requires QKI-mediated alternative splicing of Integrin Alpha-7 (Itga7)

Contact Info

Product

Resources

About