Effect of cell disruption methods on the extraction of bioactive metabolites from microalgal biomass

Stirk, Wendy A.; Balint, Peter J.; Vambe, McMaster; Lovász, Csaba; Molnár, Zoltan; Staden, J. Van; Ördög, Vince

doi:10.1016/j.jbiotec.2019.10.012

Cited by 66 publications

(38 citation statements)

References 36 publications

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“…Choi reported that the free phenolic content of shiitake (29.0 mg/100 g) increased to 38.3 and 54.6 mg/100 g FW aer heat treatment for 15 min and 30 min, respectively, at 121 C. 12 Phenolic compounds are more easily released because heat treatment breaks the cell walls of plants and increases their extractability. 24,25 Catechin has eight monomers, including epigallocatechin gallate (EGCG), gallocatechin gallate (GCG), epigallocatechin (EGC), epicatechin (EC), epicatechin gallate (ECG), catechin gallate (CG), catechin (C) and (À)-gallocatechin (GC). 26 (À)-Gallocatechin is one of 8 avanol catechins.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the phenolic profiles and physicochemical properties of different varieties of thermally processed canned lychee pulp

Wang

Shu

et al. 2020

RSC Adv.

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Section: Discussionmentioning

confidence: 99%

Comparison of the phenolic profiles and physicochemical properties of different varieties of thermally processed canned lychee pulp

Wang

Shu

et al. 2020

RSC Adv.

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“…Authors such as Stirk et al (2020) [40] determined the auxin-like activity of different microalgae extracts from Chlorella sp., Chlorella vulgaris and Scenedesmus acutus obtained after different cell disruption methods by applying a mung bean bioassay [41] different from the one applied in this work. The authors determined that some method of cell disruption is necessary to increase the extraction yield of the bioactive molecules for these microalgae species as sonication or ball mill.…”

Section: Responsementioning

confidence: 99%

Biostimulant Potential of Scenedesmus obliquus Grown in Brewery Wastewater

et al. 2020

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Microalgae are microorganisms with the capacity to contribute to the sustainable and healthy food production, in addition to wastewater treatment. The subject of this work was to determine the potential of Scenedesmus obliquus microalga grown in brewery wastewater to act as a plant biostimulant. The germination index of watercress seeds, as well as the auxin-like activity in mung bean and cucumber, and in the cytokinin-like activity in cucumber bioassays were used to evaluate the biostimulant potential. Several biomass processes were studied, such as centrifugation, ultrasonication and enzymatic hydrolysis, as well as the final concentration of microalgal extracts to determine their influence in the biostimulant activity of the Scenedesmus biomass. The results showed an increase of 40% on the germination index when using the biomass at 0.1 g/L, without any pre-treatment. For auxin-like activity, the best results (up to 60% with respect to control) were obtained at 0.5 g/L of biomass extract, after a combination of cell disruption, enzymatic hydrolysis and centrifugation. For cytokinin-like activity, the best results (up to 187.5% with respect to control) were achieved without cell disruption, after enzymatic hydrolysis and centrifugation at a biomass extract concentration of 2 g/L.

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“…In the present study, the initial rooting activity (T0 and T4) achieved with the microalgal suspension prepared by gentle shaking can be attributed to the release of low molecular weight and water soluble compounds through the cell membranes that were damaged by freeze-drying. Previous experiments with two Chlorella and a Scenedesmus strain showed that freeze-drying did not disrupt the cell walls but damaged the cell membranes, increasing the membrane permeability compared to living cells (Stirk et al 2020 ). Rooting activity of the C. vulgaris biomass significantly declined over time with storage up to T9 (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The mungbean rooting assay was selected for the present study to monitor the growth-promoting activity of the C. vulgaris suspensions over time. This assay has previously been used to measure the activity of microalgal suspensions, including Chlorella strains (Stirk et al 2020 ) as well as activity of other natural plant biostimulants such as seaweed-derived extracts (Stirk and van Staden 1997 ), eckol (a phlorotannin) in the seaweed Ecklonia maxima (Rengasamy et al 2015 ), and karrikinolide — a smoke-derived butenolide (Jain et al 2008 ). In the present study, the initial rooting activity (T0 and T4) achieved with the microalgal suspension prepared by gentle shaking can be attributed to the release of low molecular weight and water soluble compounds through the cell membranes that were damaged by freeze-drying.…”

Section: Discussionmentioning

confidence: 99%

“…1 ), suggesting degradation of the active metabolites. Highest rooting was previously achieved in the mungbean bioassay using freeze-dried Chlorella biomass compared to sonicated or ball milled biomass which caused more extensive disruption to the cell walls (Stirk et al 2020 ). This was attributed to faster oxidation and degradation of the active compounds in the more extensively damaged cells (Stirk et al 2020 ).…”

Section: Discussionmentioning

confidence: 99%

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Effect of storage on plant biostimulant and bioactive properties of freeze-dried Chlorella vulgaris biomass

et al. 2021

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Microalgae are potential plant biostimulants and biocontrol agents. A major hurdle towards their commercialization is the production of large volumes of biomass at the correct time of year. Secondary metabolites are unstable and the “shelf-life” of bioactive microalgal biomass needs to be investigated. The aim of the study was to investigate the effects of storage conditions on freeze-dried microalgae to determine how long the biomass retained its growth promoting and bioactive properties under various temperature and light conditions. Chlorella vulgaris biomass was stored in the dark at − 70 °C, 10 °C, and 25 °C and in the light at 25 °C. Samples were tested every 3–4 months for 15 months. Storage time significantly influenced the rate of change in the bioactivity in the C. vulgaris biomass with storage temperature also having some effect. Rooting activity decreased in the mungbean rooting assay over time up to 12 months and then increased slightly. Antimicrobial activity increased against Staphylococcus aureus and Escherichia coli for up to 12 months and then declined. Antioxidant activity measured in the DPPH assay remained relatively stable for up to 12 months and then significantly decreased with longer storage. The change in bioactivity over time was attributed to the gradual breakdown of the rigid cell wall of C. vulgaris , thereby improving extraction efficiency but exposing the secondary metabolites to oxygen, thus quickening their degradation. Biomass produced for commercial purposes requires preliminary validation as the results of the present study showed that bioactive compounds are susceptible to degradation over time. Supplementary Information The online version contains supplementary material available at 10.1007/s10811-021-02596-9.

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Effect of cell disruption methods on the extraction of bioactive metabolites from microalgal biomass

Cited by 66 publications

References 36 publications

Comparison of the phenolic profiles and physicochemical properties of different varieties of thermally processed canned lychee pulp

Comparison of the phenolic profiles and physicochemical properties of different varieties of thermally processed canned lychee pulp

Biostimulant Potential of Scenedesmus obliquus Grown in Brewery Wastewater

Effect of storage on plant biostimulant and bioactive properties of freeze-dried Chlorella vulgaris biomass

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