Effect of Cell Differentiation for Neuroblastoma by Vitamin K Analogs

Nakayama, Toshimitsu; Asami, Satoru; Ono, Shin-ichi; Miura, Motofumi; Hayasaka, Masatoshi; Yoshida, Yoshikazu; Toriyama, Masaharu; Motohashi, Shigeyasu; Suzuki, Takashi

doi:10.1093/jjco/hyp011

Cited by 9 publications

(9 citation statements)

References 17 publications

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“…Antiproliferative effect of M:SO combination was attenuated by genistein and DTT SO is a protein phosphatase inhibitor, while M increases protein phosphorylation [38][39][40]. Both substances are known to induce ROS production.…”

Section: Resultsmentioning

confidence: 97%

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Cytotoxic effect of menadione and sodium orthovanadate in combination on human glioma cells

et al. 2011

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Gliomas are the most common primary brain tumor, and their treatment is still a challenge. Here, we evaluated the antiproliferative effect of a novel combination of two potent oxidative stress enhancers: menadione (M) and sodium orthovanadate (SO). We observed both short-term and prolonged growth inhibitory effects of M or SO alone as well as in combination (M:SO) on DBTRG.05MG human glioma cells. A stronger antiproliferative effect was observed in the short-term proliferation assay with the M:SO combination compared to either investigated agent alone. In the long-term proliferation assay, a 10-day exposure to M:SO at concentrations of 10 μM:17.5 μM or 17.5 μM:10 μM was enough to kill 100% of the cells; no cell regrowth was observed after re-incubation in drug-free media. When used in combination, the single concentration of M and SO could be decreased by 2.5- to 5-fold of those used for each experimental drug alone and still obtain a similar antiproliferative effect. The underlying molecular mechanism was investigated by co-incubating M:SO with dithiothreitol (DTT) and genistein. Both substances partially neutralized the effects of the M:SO combination, showing additive effects. This observation suggests a role of oxidative stress and tyrosine kinase stimulation in the M:SO cytotoxic effect. Our results indicate that M:SO combination is an attractive alternative for glioma treatment that encourages further study. The neutralizing effects of genistein and DTT reveal a possibility for their use in the minimization of potential M:SO systemic toxicity.

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Section: Resultsmentioning

confidence: 97%

“…M has been shown to have growth inhibitory effects on many types of human tumor cells in vitro [19,21]. It also inhibits PTP activity [40]. M might be a strong candidate for malignant glioma treatment [19] due to its low toxic effects and its retained effectiveness on multidrug-resistant cancer cell lines [19,48].…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic effect of menadione and sodium orthovanadate in combination on human glioma cells

et al. 2011

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“…As has been shown, NGF produces an outgrowth of neurites from ganglia (7,(9)(10)(11) and neural cell lines (12)(13)(14)(15)(16).…”

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confidence: 86%

Overexpression of nerve growth factor in peritoneal fluid from women with endometriosis may promote neurite outgrowth in endometriotic lesions

Arellano

Arnold

Vercellino

et al. 2011

Fertility and Sterility

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“…8) 2-[(2,3-Dihydroxy)propylthio]-3-methyl-1,4-naphthoquinone (VK3-COOH) reportedly induces the differentiation of neuroblastoma cells. 9) 2-[(2-Hydroxy)ethylthio]-3-methyl-1,4-naphthoquinone (VK3-OH) inhibits the proliferation of human renal cancer cell by inhibiting cdc25 phosphatase and inducing apoptosis. In addition, VK3-OH induced the up-regulation of Bax and down-regulation of B-cell lymphoma-2 (Bcl-2) and Bcl-xL.…”

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confidence: 99%

Vitamin K3 Analogs Induce Selective Tumor Cytotoxicity in Neuroblastoma

Kitano

Yoda

Tabata

et al. 2012

Biological & Pharmaceutical Bulletin

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Effect of Cell Differentiation for Neuroblastoma by Vitamin K Analogs

Abstract: Vitamin K3 analogs may have potential as clinical therapeutic agents for NB.

Cited by 9 publications

References 17 publications

Cytotoxic effect of menadione and sodium orthovanadate in combination on human glioma cells

Cytotoxic effect of menadione and sodium orthovanadate in combination on human glioma cells

Overexpression of nerve growth factor in peritoneal fluid from women with endometriosis may promote neurite outgrowth in endometriotic lesions

Vitamin K3 Analogs Induce Selective Tumor Cytotoxicity in Neuroblastoma

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