Effect of cations on IAA‐induced proton excretion in the xylem of <i>Vigna unguiculata</i>

Mizuno, Akiko; Katou, Kiyoshi

doi:10.1111/j.1399-3054.1992.tb05805.x

Cited by 5 publications

(2 citation statements)

References 21 publications

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“…As a result the pH difference was diminished to 1.4 pH units. This fact was also demonstrated by perfusion of solutions through the apoplast of hypocotyl segments (Mizuno and Katou 1992). It was shown that the auxin-induced acidification of the cell wall compartment could only be measured clearly when cations had been added to the perfusion medium.…”

Section: The Cation Exchanger "Cell Wall" Adulterates Ph Valuesmentioning

confidence: 94%

“…In the course of these perfusion experiments, it was also observed that the auxin-induced acidification of the xylem exudate was considerably enhanced when cations (e.g., K +) were added to the perfusion solution. From this effect it was deduced that H + ions secreted into the apoplast as a result of an auxin action were first trapped on the negatively charged cell wall constituents and could only move into the perfusion medium when cations (K + ) were available for an exchange with H + (Mizuno and Katou 1992). The authors concluded that the binding of H + to the negatively charged wall molecules was the reason that the IAA-induced acidification of the bathing medium was not always observed when tested on diverse plant tissues.…”

Section: Hyperpolarization Indicator For the Action Of Auxinmentioning

confidence: 99%

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Role of the plasma membrane H + -ATPase in auxin-induced elongation growth: historical and new aspects

Hager

2003

Journal of Plant Research

377

315

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This article will cover historical and recent aspects of reactions and mechanisms involved in the auxin-induced signalling cascade that terminates in the dramatic elongation growth of cells and plant organs. Massive evidence has accumulated that the final target of auxin action is the plasma membrane H(+)-ATPase, which excretes H(+) ions into the cell wall compartment and, in an antiport, takes up K(+) ions through an inwardly rectifying K(+) channel. The auxin-enhanced H(+) pumping lowers the cell wall pH, activates pH-sensitive enzymes and proteins within the wall, and initiates cell-wall loosening and extension growth. These processes, induced by auxin or by the "super-auxin" fusicoccin, can be blocked instantly and specifically by a voltage inhibition of the H(+)-ATPase due to removal of K(+) ions or the addition of K(+)-channel blockers. Vice versa, H(+) pumping and growth are immediately switched on by addition of K(+) ions. Furthermore, the treatment of segments either with auxin or with fusicoccin (which activates the H(+)-ATPase irreversibly) or with acid buffers (from outside) causes an identical transformation and degradation pattern of cell wall constituents during cell-wall loosening and growth. These and other results described below are in agreement with the acid-growth theory of elongation growth. However, objections to this theory are also discussed.

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Section: The Cation Exchanger "Cell Wall" Adulterates Ph Valuesmentioning

confidence: 94%

Section: Hyperpolarization Indicator For the Action Of Auxinmentioning

confidence: 99%

Role of the plasma membrane H + -ATPase in auxin-induced elongation growth: historical and new aspects

Hager

2003

Journal of Plant Research

377

315

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Regulation of plant elongation growth by surface and xylem proton pumps

Mizuno

Katou

1996

J. Plant Res.

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Molecular cloning of yieldins regulating the yield threshold of cowpea cell walls: cDNA cloning and characterization of recombinant yieldin

Okamoto-Nakazato

Takahashi

Kido

et al. 2000

Plant Cell & Environment

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ABSTRACTcDNA for yieldin of Vigna unguiculata L. was cloned with reverse transcriptase-polymerase chain reaction using synthetic oligonucleotides as primers. The primers were designed on the basis of the N-terminal amino acid sequence of the yieldins isolated from the wall preparation of cowpea hypocotyls. The 1·2 kbp cDNA for yieldin contained an open reading frame of 981 base pairs, encoding 327 amino acids including 23 amino acids as a putative signal sequence. An homology search of the deduced amino acid sequence revealed that the yieldin was homologous to acidic class III endochitinases (EC 3·2.1·14) and concanavalin B. A cDNA fragment containing the yieldincoding region was introduced to Escherichia coli cells using an expression vector to express the recombinant protein. The recombinant yieldin was obtained from the recombinant E. coli and its effect on the wall mechanical properties was examined by reconstitution experiments. The recombinant yieldin fully restored the acid-induced change of the yield threshold tension (y) of heat denatured glycerinated hollow cylinders (GHCs) of cowpea hypocotyls. Northern hybridization analysis revealed that the yieldin mRNA was expressed mainly in the rapid and moderate elongation region of the etiolated hypocotyl of Vigna unguiculata L.

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Effect of cations on IAA‐induced proton excretion in the xylem of Vigna unguiculata

Cited by 5 publications

References 21 publications

Role of the plasma membrane H + -ATPase in auxin-induced elongation growth: historical and new aspects

Role of the plasma membrane H + -ATPase in auxin-induced elongation growth: historical and new aspects

Regulation of plant elongation growth by surface and xylem proton pumps

Molecular cloning of yieldins regulating the yield threshold of cowpea cell walls: cDNA cloning and characterization of recombinant yieldin

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