Effect of cationic lipid and matrix lipid composition on solid lipid nanoparticle-mediated gene transfer

Tabatt, Kerstin; Sameti, Mohammad; Olbrich, Carsten; Müller, Rainer H.; Lehr, Claus‐Michael

doi:10.1016/j.ejpb.2003.10.015

Cited by 126 publications

(60 citation statements)

References 23 publications

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“…Higher zeta potential coupled with smaller size may be responsible for higher transfection efficiency of PEI-pDNA. This data is in agreement with other reports that stated in the absence of endosomolytic agents such as chloroquine, gene transfer efficiency mediated by SLN-derived gene vectors is lower than PEI 25 kDa (14,18,20).…”

Section: Percent Of Cells Expressed Gfpsupporting

confidence: 93%

“…Application of cetylpalmitate and Tween 80 for SLN formulation was reported elsewhere (14,15,16). We used a slightly modified method to prepare SLN formulation while different process variables were evaluated in an experimental design matrix consisted of 9 runs (3 2 ) which were obtained by Design-Expert 6.0.6 statistical software.…”

Section: Preparation Of Csln Formulationsmentioning

confidence: 99%

“…As a carrier system, solid lipid nanoparticles have many features that are valuable for nanomedicine. So far, SLNs have been investigated as delivery systems mostly for drugs and rarely for genes (14)(15)(16)(18)(19)(20). In both cases, this delivery system is reported to proceed more efficiently compared to the drug or gene alone.…”

Section: Percent Of Cells Expressed Gfpmentioning

confidence: 99%

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Cationic Solid Lipid Nanoparticles Loaded by Cystein Proteinase Genes as a Novel anti-Leishmaniasis DNA Vaccine Delivery System: Characterization and in vitro Evaluations

et al. 2010

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-Purpose. Leishmaniasis is a major health problem in many tropical and sub-tropical countries and development of a safe and easily-available vaccine has high priority. Although several antigens potentially capable of inducing protective immunity have been studied, in the absence of pharmaceutical industry interest they have remained as fine publications only. Amongst them, Cathepsin L-like cysteine proteinases (CPs) have received considerable attention and type I and II CPs have been used in a heterologous prime-boost vaccination regime for experimental visceral leishmaniasis in dogs. Due to the promising results of the mentioned vaccination regime, we aimed to evaluate cationic solid lipid nanoparticles (cSLNs) for in vitro delivery of cpa, cpb and cpb CTE intended to be used as a cocktail DNA vaccine in our forthcoming studies. Methods: cSLNs were formulated of cetyl palmitate, cholesterol, DOTAP and Tween 80 via melt emulsification method followed by high shear homogenization. Different formulations were prepared by anchoring pDNAs on the surface of cSLNs via charge interaction. The formulations were characterized according to their size and zeta potential as well as pDNA integrity and stability against DNase I treatment. Lipoplexes' cytotoxicity was investigated on COS-7 cells by MTT test. The effect of the DOTAP:pDNA ratio on protection ability and cytotoxicity was also studied. In vitro transfection efficiency was qualified by florescent microscopy and quantified using flow cytometry technique. Results: cSLN-pDNA complexes were formulated with suitable size and zeta potential. Efficiency/cytotoxicity ratio of cSLN-pDNAs formulations was comparable to linear PEI-25kDa-pDNAs polyplexes while exhibiting significantly lower cytotoxicity. Conclusion: Tested formulations were able to deliver immunogenic CP genes efficiently. This data proves the ability of this system as a promising DNA vaccine carrier for leishmaniasis to cover the main drawback of naked pDNA delivery that is rapidly elimination from the circulation.

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Section: Percent Of Cells Expressed Gfpsupporting

confidence: 93%

Section: Preparation Of Csln Formulationsmentioning

confidence: 99%

Section: Percent Of Cells Expressed Gfpmentioning

confidence: 99%

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Cationic Solid Lipid Nanoparticles Loaded by Cystein Proteinase Genes as a Novel anti-Leishmaniasis DNA Vaccine Delivery System: Characterization and in vitro Evaluations

et al. 2010

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“…3,7 The DNA binding and in vitro transfection efficiency for cetyl palmitate cLNP, stabilized by TW, SP and DOTAP, and liposomes prepared from the same stabilizers, were found to be very similar. 8 In previous studies Compritol s ATO 888 (a mixture of mono-, di-and triglyerides of mainly behenic fatty acids), 3,7,[9][10][11] Precirol s ATO 5 (glycerol distearate), 12 cetyl palmitate 8,9 or fatty a Professur für Nanomaterialcharakterisierung (Streumethoden), Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstr. 3, 91058 Erlangen, Germany.…”

Section: Introductionmentioning

confidence: 99%

“…E-mail: tobias.unruh@fau.de b German Engineering Materials Science Centre (GEMS) at Heinz Maier-Leibnitz acids like oleic and stearic acid were used as matrix lipids. Nonionic stabilizers like TW(+SP) and PLX and different cationic co-stabilizers like CPYC, 3,9 CTAB, 9 EQ1, 3,9 DODAB [9][10][11] and DOTAP 8,9,12 were used to stabilize the cLNP and to equip them with a positive charge.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the structure of nanocomposites of triglyceride platelets and DNA

Schmiele

Knittel

Unruh

et al. 2015

Phys. Chem. Chem. Phys.

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DNA-complexes with platelet-like, cationically modified lipid nanoparticles (cLNPs) are studied with regard to the formation of nanocomposite structures with a sandwich-like arrangement of the DNA and platelets. For this purpose suspensions of platelet-like triglyceride nanocrystals, stabilized by a mixture of two nonionic (lecithin plus polysorbate 80 or poloxamer 188) and one cationic stabilizer dimethyldioctadecylammonium (DODAB), are used. The structure of the platelets in the native suspensions and their DNA-complexes, ranging from the sub-nano to the micron scale, is investigated with small-and wideangle scattering (SAXS, SANS, WAXS), calorimetry, photon correlation spectroscopy, transmission electron microscopy and computer simulations. The appearance of strong, lamellarly ordered peaks in the SAXS patterns of the DNA-complexes suggests a stacked arrangement of the nanocrystals, with the DNA being partially condensed between the platelets. This finding is supported with computer simulated small-angle scattering patterns of nanocrystal stacks, which can reproduce the measured small-angle scattering patterns on an absolute scale. The influence of the choice of the nonionic stabilizers and the amount of the cationic stabilizer DODAB on the structure of the native suspensions and the inner structure of their DNA-complexes is studied, too. Using high amounts of DODAB, lecithins with saturated acyl chains and polysorbate 80 instead of poloxamer 188 produces thinner nanocrystals, and thus decreases their repeat distances in the nanocomposites. Such nanocomposites could be of interest as DNA carriers, where the triglyceride platelets protect the sandwiched DNA from degradation.

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