EFFECT OF CATALASE AND CULTURAL CONDITIONS ON GROWTH OF
            <i>BEGGIATOA</i>
            ,

Burton, Sheril D.; Morita, Richard Y.

doi:10.1128/jb.88.6.1755-1761.1964

Cited by 47 publications

(25 citation statements)

References 13 publications

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“…Like their strains, clone 75-2a is capable of gliding motility, grows on medium containing acetate and dilute complex organics and on medium containing acetate and sulfide, and deposits internal granules of S°from sulfide. Clone 75-2a and their strains are also alike in that both deposit PHB granules when grown on media containing moderate amounts of acetate and 1 2 Pyruvate (mm) others (3). The possibility of synergistic interactions between catalase and organic substrates was further tested by determining growth yields of Beggiatoa sp.…”

Section: Resultsmentioning

confidence: 99%

“…Combining this with the above results implies that growth yield on medium containing acetate, yeast extract, and catalase is simply the sum of yields on the three separate components. The fifth entry in the second line of Table 3 represents the yield of clone 75-2a on the "best growth" medium of Burton and Morita (3). For their strain of Beggiatoa sp., they claimed a 24-fold enhancement of yield on acetate + yeast extract medium when catalase was added.…”

Section: Resultsmentioning

confidence: 99%

“…The inability of clone 75-2a to utilize the amino acids or sugars tested (Table 3) either singly or together with acetate is consistent with the low growth yields from yeast extract. Lack of ability in Beggiatoa species to utilize sugars has been pointed to repeatedly by others (3,23,26). Amino acids have been reported to enhance growth (22,23) and to have no influence (10,26).…”

Section: Downloaded Frommentioning

confidence: 88%

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Organic nutrition of Beggiatoa sp

Nelson¹,

Castenholz²

1981

J Bacteriol

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Culture OH-75-B of Beggiatoa sp. differed significantly from any described previously in its utilization of organic carbon and reduced sulfur compounds. It deposited internal sulfur granules characteristic of Beggiatoa sp. with either sulfide or thiosulfate in the medium. This strain (OH-75-B, clone 2a) could be grown in agitated liquid cultures on mineral medium with acetate as the only source of organic carbon. The resultant growth yields and rates were comparable to those for typical heterotrophs. Of the other simple organic compounds tested, only pyruvate, lactate, or ethanol could singly support the growth of this strain. Single sugars or amino acids neither supported growth nor enhanced it when added to acetate-containing medium. In contrast, compounds of the tricarboxylic acid cycle enhanced growth yields when tested in concert with acetate. These and fluoroacetate inhibition results indicate that Beggiatoa sp. possesses a functional tricarboxylic acid cycle. Poor yields characterized the growth of this strain on dilute yeast extract medium, and higher concentrations of yeast extract proved inhibitory. The enzyme catalase, contrary to the findings of others, had no synergistic influence on growth yields when added to medium containing yeast extract or acetate or both.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Downloaded Frommentioning

confidence: 88%

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Organic nutrition of Beggiatoa sp

Nelson¹,

Castenholz²

1981

J Bacteriol

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“…Many environmental factors contribute to the inability of micro-organisms to replicate on laboratory media. In particular, the accumulation of reactive oxygen species (ROS) in the growth media has been shown to be deleterious to the recovery of injured, stressed, or microaerophilic bacteria, while the addition of ROS-degrading compounds has been shown to support the cultivation of these micro-organisms (Burton and Morita 1964;Kautter 1976, 1977;Martin et al 1976;Padgett et al 1982;Cox et al 1990;Hood et al 1990;Olson et al 2000;Stevenson et al 2004). These studies suggest that the addition of ROS-degrading compounds to culture media may permit the cultivation of a greater percentage of the environmental microbial community than is currently contained within repositories.…”

Section: Introductionmentioning

confidence: 99%

Detection of Actinobacteria cultivated from environmental samples reveals bias in universal primers

Farris

Olson

2007

Lett Appl Microbiol

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Aims: The aims of this study were to develop media to cultivate actinomycetes, screen the resulting isolates with Actinobacteria‐specific primers, and examine the efficacy of detection of the actinobacterial isolates with universal primers. Methods and Results: Soil‐extract medium was developed for a terrestrial bluff environment. Recovered isolates were subjected to polymerase chain reaction (PCR) with taxon‐specific primers to identify Actinobacteria. Universal bacterial primers 24f and 1492r (modified and original versions) were used to amplify the 16S rRNA gene from the putative Actinobacteria. While both reverse primers failed to provide amplification products from 20% to 50% of the isolates, the 1492r primer detected Actinobacteria more effectively than 1492r‐mod. The region of the gene containing the annealing site for the 1492r primers from 15 isolates that failed to amplify showed no differences in nucleotide sequence to the original 1492r primer. Conclusions: Universal 16S rRNA gene primers are not capable of amplifying this gene from all bacteria within an environmental sample. Some Actinobacteria may share 100% sequence similarity to universal primers but remain undetected. Significance and Impact of the Study: These findings are important for studies of particular taxa in environmental samples where reactions utilizing universal primers may not reveal the extent of their presence and diversity.

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“…The use of soil extract and the incorporation of catalase into the medium reduces the length of time required for enrichment. Catalase has been shown to stimulate the growth of Beggiatoa by Burton & Morita (1964). The stimulatory effect of soil extract may be attributed to the presence of growth promoting substance(s) in the mud.…”

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confidence: 99%