Effect of castration on male rabbit lower urinary tract tissue enzymes

Juan, Yung‐Shun; Önal, Bülent; Broadaway, Samuel; Cosgrove, Julia; Leggett, Robert E.; Whitbeck, Catherine; De, Elise; Sokol, Rebekah; Levin, Robert M.

doi:10.1007/s11010-007-9415-8

Cited by 23 publications

(17 citation statements)

References 22 publications

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“…At the molecular level, UB contraction is essentially dependant on the adenosine triphosphate (ATP) concentration in the bladder wall, as well as mitochondrial oxidative enzymes activity for energy production (Juan et al 2007). Castration decreases noticeably the activities of the mitochondria specific enzyme, citrate synthase of the UB muscle and mucosa (Juan et al 2007) which could be corrected by TRT. Nitric oxide (NO) is also one of the mediators of dilatation of the bladder neck and urethra (McVary 2006) by its smooth muscle relaxing effect in the urogenital system (Pradidarcheep 2008).…”

Section: Discussionmentioning

confidence: 99%

Effect of testosterone therapy on the urinary bladder in experimental hypogonadism of rats

Abdel-Hamid

Ali

2015

J Mol Hist

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Testosterone (T) deficiency is prevalent particularly in elderly men and lead to physical and sexual morbidities. Although low levels of T are associated with low urinary tract symptoms, the correlation between T deficiency and bladder dysfunction is not clearly identified. The aim of this study was to investigate the effect of high dose testosterone replacement therapy (TRT) on the histological structure of the UB in castrated rats. Twenty-five adult male rats were divided into three groups: control, castrated and castrated + TRT. T was administrated in high dose (100 mg/kg) two intramuscular injections/week for 60 days. UB sections were prepared and stained with H&E, Masson's trichrome and immunohistochemical detection of Cytokeratin 20 (Ck20). All data were morphometrically and statistically analyzed. In castrated group, significant atrophy of the urothelium (P < 0.001) accompanied with widening of the corium were observed. The smooth muscle appeared thin with marked increase in the collagen fibers. On treating the castrated group with TRT, atypical Ck20 expression as well as significant increase in urothelial thickness (P < 0.05) and smooth muscle/collagen ratio (P < 0.001) were detected. In castrated rat model, high dose TRT has a positive effect on the UB smooth muscle rather than the urothelium which acquired atypical patterns.

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Section: Discussionmentioning

confidence: 99%

Effect of testosterone therapy on the urinary bladder in experimental hypogonadism of rats

Abdel-Hamid

Ali

2015

J Mol Hist

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“…The hypothesis that testosterone may influence the micturition reflex has been shown in several animal studies. Juan et al, (2007) reported that choline acetyltransferase activity, the indicator of cholinergic innervation, was significantly decreased in the bladder base and urethra 2 weeks after castration, indicative of the importance of testosterone in maintaining cholinergic nerve function. Lastly, testosterone increases angiotensin II levels and plays a role in the subsequent increase in contractility of vascular smooth muscles (Flynn et al, 1990).…”

Section: Discussionmentioning

confidence: 99%

Relationship between serum testosterone and nocturia in men without benign prostate enlargement

et al. 2016

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SUMMARYRecent studies have focused on the relationship between nocturia and serum testosterone because testosterone is thought to be an important factor of prostate growth. However, it remains unclear whether altered serum concentrations of testosterone is associated with an increased risk of nocturia because patients who were taking diuretics or who had a large prostate, which may precipitate nocturia, were not excluded from most previous studies. We analyzed the clinical records of 596 non-benign prostatic enlargement (BPE) male patients to explore the relationship between serum total testosterone and nocturia. All patients were evaluated using a serum prostate-specific antigen (PSA) assay, measurement of serum total testosterone, transrectal ultrasonography, uroflowmetry, and a compilation of the International Prostate Symptom Score (IPSS) and International Index of Erectile Function (IIEF) questionnaires. Nocturia was defined as ≥2 nocturnal voiding episodes. The number of nocturia episodes was assessed using IPSS question 7. To evaluate the effect of serum testosterone on nocturia, multivariate regression analysis was performed including the covariates of age, IPSS, IIEF score, body mass index, PSA, prostate volume, and maximal urine flow rate. Based on multivariate linear analysis, serum testosterone level was not significantly associated with the severity of nocturia. However, with regard to the relationship between prevalence of nocturia and serum testosterone, prevalence of nocturia was significantly positively associated with age (OR = 1.048, p = 0.005), total IPSS (OR = 1.217, p < 0.001), and testosterone level (OR = 1.150, p = 0.041). Therefore, in men without an enlarged prostate, testosterone may play an opposing role in the etiology of nocturia.

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“…The phasic response depends on the adenosine triphosphate (ATP) concentration in the bladder wall, whereas the tonic phase requires mitochondrial oxidative activity to generate energy. Castration of adult male rabbits resulted in a significant decrease in the activities of the mitochondria specific enzyme, citrate synthase of the bladder body and base, muscle and mucosa, urethra and corpora, while choline acetyl-transferse activity and calcium ATPase activity showed different responses depending on the sites (Juan et al, 2007). Preliminary evidence indicates that men with LUTS benefit from testosterone treatment, and pilot studies have also shown that testosterone therapy has a positive effect on LUTS in lateonset hypogonadism.…”

Section: Testosterone Deficiency Linked To Lutsmentioning

confidence: 97%

“…In the rabbit bladder outlet obstruction study, bladder dysfunction is mainly mediated by three cellular processes; 1) progressive denervation, 2) cellular mitochondria malfunction, 3) dysregulation of intracellular calcium storage and release from the sarcoplasmic reticulum (SR). Biomarkers for these three functions are calcium adenosine triphosphatase (ATPase) for calcium release, citrate synthase for mitochondrial function, and choline acetyl-transferse for cholinergic innervations (Juan et al, 2007). Bladder contraction can be divided into phasic and tonic period.…”

Section: Testosterone Deficiency Linked To Lutsmentioning

confidence: 99%

Testosterone Deficiency Linked to Lower Urinary Tract Symptoms

Kim¹

2012

Sex Hormones

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Effect of castration on male rabbit lower urinary tract tissue enzymes

Cited by 23 publications

References 22 publications

Effect of testosterone therapy on the urinary bladder in experimental hypogonadism of rats

Effect of testosterone therapy on the urinary bladder in experimental hypogonadism of rats

Relationship between serum testosterone and nocturia in men without benign prostate enlargement

Testosterone Deficiency Linked to Lower Urinary Tract Symptoms

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