Effect of carboxymethyl groups on degradation of modified pullulan by pullulanase from Klebsiella pneumoniae

Ali, Ghina; Rihouey, Christophe; Cerf, Didier Le; Picton, Luc

doi:10.1016/j.carbpol.2012.07.039

Cited by 15 publications

(25 citation statements)

References 44 publications

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“…Apart from nitrogenase reductases, Klebsiella can also produce starch-debranching enzymes, such as pullulanases [55]. These microbes can utilize starch as the sole carbon and energy source via two metabolic routes.…”

Section: Starch Digestion and Its Relation To Gut Microbesmentioning

confidence: 99%

The Role ofKlebsiellain Crohn’s Disease with a Potential for the Use of Antimicrobial Measures

Rashid

Ebringer

Wilson

2013

International Journal of Rheumatology

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There is a general consensus that Crohn's disease (CD) develops as the result of immune-mediated tissue damage triggered by infections with intestinal microbial agents. Based on the results of existing microbiological, molecular, and immunological studies, Klebsiella microbe seems to have a key role in the initiation and perpetuation of the pathological damage involving the gut and joint tissues in patients with CD. Six different gastroenterology centres in the UK have reported elevated levels of antibodies to Klebsiella in CD patients. There is a relationship between high intake of starch-containing diet, enhanced growth of gut microbes, and the production of pullulanases by Klebsiella. It is proposed that eradication of these microbes by the use of antibiotics and low starch diet, in addition to the currently used treatment, could help in alleviating or halting the disease process in CD.

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Section: Starch Digestion and Its Relation To Gut Microbesmentioning

confidence: 99%

The Role ofKlebsiellain Crohn’s Disease with a Potential for the Use of Antimicrobial Measures

Rashid

Ebringer

Wilson

2013

International Journal of Rheumatology

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“…Pullulan (PI20) was purchased from Hayashibara Biochemical Laboratory, Japan. Number and weight average molar masses ( M n = 120,000 g mol −1 ; M w = 240,000 g mol −1 were determined using SEC/MALS/DRI technique. Alginate from Lessonia Trabeculata (M/G = 0.5; M n = 194,000 g mol −1 ; M w = 342,000 g mol −1 was obtained graciously from Cargill (Baupte, France).…”

Section: Methodsmentioning

confidence: 99%

“…Pullulanase from Klebsiella pneumoniae is an enzyme of Type I, which hydrolyses the 1,6 linkages of pullulan with an endo or exo mechanism . The optimal activity conditions of pullulanase, from Klebsiella pneumonia, have been determined as pH 5.5 and 60°C . In such conditions, a large number of pullulanase lost its activity after a few hours mainly because of a limitation of the enzyme stability.…”

Section: Introductionmentioning

confidence: 99%

Covalent immobilization of pullulanase on alginate and study of its hydrolysis of pullulan

Ali

Dulong

Gasmi

et al. 2015

Biotechnology Progress

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The immobilization of pullulanase from Klebsiella pneumoniae by grafting was investigated. Pullulanase was linked after activation of alginate via a covalent bond between the amine groups of the enzyme and the carboxylic acid groups of alginate. The immobilization yield was 60%. The activity of free pullulanase and immobilized pullulanase was followed by the quantification of reducing ends by colorimetric assay and the determination of the molar masses of the hydrolyzed pullulan by SEC/MALS/DRI. Compared to free pullulanase, the kinetics is largely slowed. The evolution of the weight average molar mass of pullulan leading to high production of shorter oligosaccharides during hydrolysis is not the same as that obtained with free enzyme. Immobilized pullulanase retained 75% and 30% of its initial activity after 24 h and 14 days of incubation at 60°C, respectively while free pullulanase lost its activity after 5 h of hydrolysis at the same temperature. The kinetic parameters of immobilized pullulanase were also investigated by isothermal titration calorimetry (ITC). The affinity of immobilized enzyme to its substrate was reduced compared to the free pullulanase due to steric hindrance and chemical links.

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“…Jiang et al [25] developed various fully bioabsorbable natural-materials-based TENGs, which were used as an in vivo voltage source to accelerate the beating rates of dysfunctional cardiomyocyte clusters and improve the consistency of cell contractions. [23] Therein, many other polysaccharides [26][27][28][29] with unique properties are supposed to be researched and optimized for the better electric performance and bioapplications of the bio-TENGs.Pullulan, produced by the yeast-like funguses, [30] is a linear and unbranched micro-organismal polysaccharide with maltotriose repeating units connected by α−(1 → 6) glycosidic bonds. [23] Therein, many other polysaccharides [26][27][28][29] with unique properties are supposed to be researched and optimized for the better electric performance and bioapplications of the bio-TENGs.…”

mentioning

confidence: 99%

“…Pullulan, produced by the yeast-like funguses, [30] is a linear and unbranched micro-organismal polysaccharide with maltotriose repeating units connected by α−(1 → 6) glycosidic bonds. [31] In the past several decades, pullulan and its derivatives have been widely applied in biomedical, [31] food, [32] pharmaceutical, [33] and petroleum industries [34,35] with the properties of nonmutagenicity, water solubility, editability, [36] nontoxicity, biodegradation, [37] biocompatibility, nonimmunogenicity, and noncarcinogenicity.…”

mentioning

confidence: 99%

A Flexible, Recyclable, and High‐Performance Pullulan‐Based Triboelectric Nanogenerator (TENG)

Ping

et al. 2019

Adv Materials Technologies

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interests on the material engineering, [10,11] structure modification, [12][13][14][15][16] and practical applications. [17][18][19][20][21] However, there are several limitations on biomedical and biowearable applications for TENGs due to the synthetic polymers and nonbiocompatibility of the majority ongoing TENGs. Thus, the natural biomaterialsbased TENGs [22] have immensely attracted the researcher's attentions with the merits of biocompatibility, low cost, and plentiful raw materials. [23][24][25] Wang et al. [24] reported a biodegradable and flexible chitosanbased TENG (chitosan was derived from chitin) and explored the feasibility of laser processing of constituent materials. Jiang et al. [25] developed various fully bioabsorbable natural-materials-based TENGs, which were used as an in vivo voltage source to accelerate the beating rates of dysfunctional cardiomyocyte clusters and improve the consistency of cell contractions. Generally, the natural biomaterials, prepared into bio-TENGs, are mainly divided into two strategies, including natural proteins and polysaccharides. [23] Therein, many other polysaccharides [26][27][28][29] with unique properties are supposed to be researched and optimized for the better electric performance and bioapplications of the bio-TENGs.Pullulan, produced by the yeast-like funguses, [30] is a linear and unbranched micro-organismal polysaccharide with maltotriose repeating units connected by α−(1 → 6) glycosidic bonds. [31] In the past several decades, pullulan and its derivatives have been widely applied in biomedical, [31] food, [32] pharmaceutical, [33] and petroleum industries [34,35] with the properties of nonmutagenicity, water solubility, editability, [36] nontoxicity, biodegradation, [37] biocompatibility, nonimmunogenicity, and noncarcinogenicity. To our knowledge, there is no research about TENGs or wearable devices fabricated with pullulan. Moreover, the excellent properties of pullulan make it possible to design and adjust the composite of the pullulan film, which is more suitable for TENGs and the biowearable devices. Here, we develop a flexible, recyclable, and highperformance pullulan-based TENG to harvest and transform mechanical energy into electric energy. The physical and chemical characterizations and output performance are investigated to optimize the composite and thickness of the pullulan-based TENGs. Then, the feasibility of the pullulan-based TENGs as the wearable device is explored. Finally, we investigate the recycling performance of the pullulan-based TENG, evaluated by the open-circuit voltage of the as-fabricated TENGs.The developments of natural biomaterials-based triboelectric nanogenerators (TENGs) have been emerged with the extensive and promising requirements in biomedical and biowearable fields due to the properties of renewability, cost-effectivity, biocompatibility, and biodegradability. In this work, a flexible, recyclable, and high-performance pullulan-based TENG, fabricated with pullulan and other additives, is developed. Based on the ope...

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Effect of carboxymethyl groups on degradation of modified pullulan by pullulanase from Klebsiella pneumoniae

Cited by 15 publications

References 44 publications

The Role ofKlebsiellain Crohn’s Disease with a Potential for the Use of Antimicrobial Measures

The Role ofKlebsiellain Crohn’s Disease with a Potential for the Use of Antimicrobial Measures

Covalent immobilization of pullulanase on alginate and study of its hydrolysis of pullulan

A Flexible, Recyclable, and High‐Performance Pullulan‐Based Triboelectric Nanogenerator (TENG)

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