Effect of carbonic anhydrase and urease on bacterial Calcium carbonate precipitation

Priya, J. Neena; Nan, M. Kan

doi:10.22376/ijpbs.2017.8.3.b609-614

Cited by 10 publications

(7 citation statements)

References 10 publications

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“…Results showed that U17 had high UA and CA activity (Table 1). Similar to our results, many bacterial strains including Bacillus and Pseudomonas with high enzyme activities showed high CaCO 3 precipitation (Dhami et al, 2014;Priya and Kannan, 2017). In addition to these results, it has been shown for the first time in our study that these enzyme activities changed in different media.…”

Section: Discussionsupporting

confidence: 91%

“…Quantitative EPMA analysis of produced CaCO 3 by Bacillus amyloliquefaciens U17 from another part of the same sample (data belonging to Figure 7). high CaCO 3 precipitation (Dhami et al, 2014;Priya and Kannan, 2017). In addition to these results, it has been shown for the first time in our study that these enzyme activities changed in different media.…”

Section: Vateritesupporting

confidence: 83%

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Precipitation and characterization of CaCO3 of Bacillus amyloliquefaciens U17 strain producing urease and carbonic anhydrase

et al. 2019

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In the present study, the properties of calcium carbonate mineralization and urease and carbonic anhydrase activities of Bacillus amyloliquefaciens U17 isolated from calcareous soil of Denizli (Turkey) were analyzed. CaCO 3 was produced in all growth phases. Strain U17 showed 0.615 ± 0.092 µmol/min/mg urease enzyme activity in calcium mineralization medium and 1.315 ± 0.021 µmol/min/mg urease enzyme activity in Luria-Bertani medium supplemented with urea, whereas it showed 36.03 ± 5.48 nmol/min/ mg carbonic anhydrase enzyme activity in CaCO 3 precipitation medium and 28.82 ± 3.31 nmol/min/mg carbonic anhydrase enzyme activity in Luria-Bertani medium supplemented with urea. The urease B protein expression level of strain U17 was detected by western blotting for the first time. The produced CaCO 3 crystals were analyzed by X-ray diffraction, X-ray fluorescence, confocal RAMAN spectrophotometer, scanning electron microscopy, and electron probe microanalyzer for the evaluation of their morphological and elemental properties. Rhombohedral vaterite and layered calcite crystals were clearly detected and verified by mineralogical analyses. All these results showed that strain U17 can be used in many engineering and geological applications due to its CaCO 3 precipitation ability.

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Section: Discussionsupporting

confidence: 91%

Section: Vateritesupporting

confidence: 83%

Precipitation and characterization of CaCO3 of Bacillus amyloliquefaciens U17 strain producing urease and carbonic anhydrase

et al. 2019

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“…In contrast to strain U2 urease, strain ATCC 6453 urease activity was higher in CPM (Table 2). Similar to our results many bacterial strains including Bacillus and Pseudomonas with high enzyme activities showed high CaCO3 precipitation [33]. Omoregie et al [22] reported that urease activities for the bacteria isolated from limestone caves had higher urease activity.…”

Section: Enzymes Activitiessupporting

confidence: 90%

Calcium Carbonate Precipitation by Urease and Carbonic Anhydrase Positive Bacteria

Bozbeyoğlu¹,

Candoğan²,

Arslan³

et al. 2020

Pamukkale J Eng Sci

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In present study, CaCO3 precipitation was examined in two ureolytic bacteria. Bacillus aerius U2 and Sporosarcina pasteurii ATCC 6453 were used as test organisms. The determination of urease and carbonic anhydrase enzyme activities were also determined. For further confirmation of the calcium carbonate mineral type produced by bacteria, XRD, SEM and EDX analysis were done. Strain U2 produced calcite and vaterite. In S. pasteurii ATCC 6453, only vaterite was found. The enzyme activity studies showed that both urease and carbonic anhydrase activities was 2-50-fold higher in S. pasteurii ATCC 6453 than B. aerius U2. Although, S. pasteurii ATCC 6453 was better option for microbial calcium carbonate precipitation (MCP) at higher temperature, by B. aerius U2 at lower temperature (<30 °C) is made possible to employ in the most geotechnical applications. Bu çalışmada, iki üreolitik bakteride CaCO3 çökelimi araştırılmıştır. Test organizması olarak Bacillus aerius U2 ve Sporosarcina pasteurii ATCC 6453 türleri kullanılmıştır. Bunların yanında, bakterilerin üreaz ve karbonik anhidraz enzim aktiviteleri de tespit edilmiştir. Bakteriler tarafından üretilen kalsiyum karbonatın mineral tipi konformasyonu için XRD, SEM ve EDX analizleri gerçekleştirilmiştir. B. aerius U2 suşu kalsit ve vaterit üretmiştir. S. pasteurii ATCC 6453 suşunda sadece vaterit bulunmuştur. Enzim aktivitesi çalışmalarının sonuçlarına göre S. pasteurii ATCC 6453, B. aerius U2'den 2-50 kat daha fazla aktivite göstermiştir. Yüksek sıcaklıklarda kalsiyum karbonat çökelimi için S. pasteurii ATCC 6453 türü tercih edilebilirken, daha geniş iklim bölgelerini kapsayan düşük sıcaklıklarda(<30 °C) B. aerius U2 türünün kullanım potansiyeli bulunmaktadır.

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“…Both enzymatic pathways, urease and carbonic anhydrase, have often been studied separately as inducing biomineralization [10,15,23,31,32] but it is possible that a coupling of these 2 ways would allow better biocalcification in some bacteria [18,19,33]. The urease pathway would provide an alkalinisation around the cells by hydrolysis of urea and the carbonic anhydrase pathway would provide an additional source of HCO 3…”

Section: Metabolic Pathways Involved In Biomineralizationmentioning

confidence: 99%

Biomineralization of calcium carbonate by marine bacterial strains isolated from calcareous deposits

Vincent

Sabot

Lanneluc

et al. 2020

Matériaux & Techniques

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Biomineralization induced by microbial enzymes, which catalyse CaCO3 precipitation, is a promising field of research for various applications in building eco-materials. Especially, this could provide an eco-friendly process for protection of coastal areas against erosion. In the present investigation, fourteen bacterial strains were isolated and characterized from both natural seawater and calcareous deposits formed on a cathodically protected steel mesh in marine environment. All of them induced calcium carbonate precipitation in various media by producing urease and/or carbonic anhydrase enzymes. The calcium carbonate minerals produced by bacteria were identified by microscopy and µ-Raman spectroscopy. In parallel, an experimental set-up, based on a column reactor, was developed to study biomineralization and microbial capacity of Sporosarcina pasteurii to form sandy agglomerate. These well-known calcifying bacteria degraded the urea present in liquid medium circulating through the column to produce calcium carbonate, which acted as cement between sand particles. The bio-bricks obtained after 3 weeks had a compressive strength of 4.2 MPa. 20% of the inter-granular voids were filled by calcite and corresponded to 13% of the total mass. We successfully showed that bio-column system can be used to evaluate the bacterial ability to agglomerate a sandy matrix with CaCO3.

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Effect of carbonic anhydrase and urease on bacterial Calcium carbonate precipitation

Cited by 10 publications

References 10 publications

Precipitation and characterization of CaCO3 of Bacillus amyloliquefaciens U17 strain producing urease and carbonic anhydrase

Precipitation and characterization of CaCO3 of Bacillus amyloliquefaciens U17 strain producing urease and carbonic anhydrase

Calcium Carbonate Precipitation by Urease and Carbonic Anhydrase Positive Bacteria

Biomineralization of calcium carbonate by marine bacterial strains isolated from calcareous deposits

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