Effect of Carbon Dioxide and Ethylene on Tuberization of Isolated Potato Stolons Cultured <i>in Vitro</i>

Mingo-Castel, Ángel M.; Negm, Fayek B.; Smith, O. E.

doi:10.1104/pp.53.6.798

Cited by 51 publications

(26 citation statements)

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“…Elsewhere (16) we reported inhibition by C2H4 of the kinetininduced tuberization of stolons cultured in vitro. Swellings promoted by kinetin were visible after 7 to 10 days in culture.…”

Section: Methodsmentioning

confidence: 99%

“…The following changes were made in relation to the methodology described previously (16). Sprouts were excised when they were 6 to 8 cm long.…”

Section: Methodsmentioning

confidence: 99%

“…Where indicated, kinetin was used at a concentration of 5 mg/i. Tuberization of sprout sections was studied by culture in medium 2 using the methodology described by Mingo-Castel et al (16). When used, CO2 and C2H4 were injected in amounts which would produce initial concentrations in the flasks of 10% (v/v) of CO2 and 5 Al/1 of C2H4.…”

Section: Methodsmentioning

confidence: 99%

“…A number of apparently contradictory reports in the literature on the role of ethylene in tuberization were discussed previously (16). Potato plants sprayed with Ethrel registered a decrease in the number of tubers/plant (12), or there was no change in either the number of tubers/plant or the weight of tubers (20).…”

mentioning

confidence: 99%

“…We have recently reported on the actions of ethylene and CO2 on tuber initiation of isolated potato stolons cultured in vitro (16) the C2H4 inhibition of tuberization in vitro. The earlier work on potato stolons has been further extended to the study of the actions of CO2 and C2H4 on tuberization of sprout sections cultured in vitro.…”

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confidence: 99%

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Studies on the Carbon Dioxide Promotion and Ethylene Inhibition of Tuberization in Potato Explants Cultured in Vitro

Mingo-Castel¹,

Smith²,

Kumamoto³

1976

Plant Physiol.

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the C2H4 inhibition of tuberization in vitro. The earlier work on potato stolons has been further extended to the study of the actions of CO2 and C2H4 on tuberization of sprout sections cultured in vitro. MATERIALS AND METHODSPlant Material and in Vitro Culture. Tubers of Solanum tuberosum L. var. Red La Soda which had been grown for seed were used in all experiments. On one occasion, var. White Rose was also tested. Sprout sections or stolons were obtained according to the technique described previously (16) with a few modifications. Excised stolons grown from axillary buds of sprout sections were surface-sterilized by immersion in a Purex bleach solution (diluted 1:10), for 2 to 3 min, before being transferred to culture flasks or test tubes. Where indicated, kinetin was used at a concentration of 5 mg/i. Tuberization of sprout sections was studied by culture in medium 2 using the methodology described by . When used, CO2 and C2H4 were injected in amounts which would produce initial concentrations in the flasks of 10% (v/v) of CO2 and 5 Al/1 of C2H4. Filter paper was inserted in small (16 x 41 mm) test tubes before autoclaving the flasks containing the culture medium. Trapping solutions (16) for CO2 and/or C2H4 were dispensed later in the small test tubes.Time Course Experiments of CO2 Action. The following changes were made in relation to the methodology described previously (16). Sprouts were excised when they were 6 to 8 cm long. Two ml of sterile H20 were added to culture flasks on top of the solidified culture medium 2 to prevent desiccation of the internal atmosphere in the flasks produced by absorption of H20 by a 20% (w/v) KOH solution (CO2 trapping solution).One-third of the available stolons was cultured in medium 2 with 10% (v/v) CO2 in the absence of C2H4 for 30 days. Another third was cultured in the same conditions for 1, 2, 3, or 5 days and then transferred (designated as tr) to medium 2 in a flask covered with a Morton stainless steel closure which allowed exchange with external air (open control), or the flasks were purged (designated as p) under vacuum in the sterile transfer hood for 3 min and then covered with a Morton closure. After purging, the CO2 concentration in the flasks was less than 0.2%. The small test tube containing mercuric perchlorate solution was removed from the flask at this time. The final third of the stolons was cultured in medium 2 in an atmosphere in equilibrium with the exterior (open control).Extraction and assay of starch synthetase from potato tubers were based on the procedure described by Hawker et al. (10).

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“…Elsewhere (16) we reported inhibition by C2H4 of the kinetininduced tuberization of stolons cultured in vitro. Swellings promoted by kinetin were visible after 7 to 10 days in culture.…”

Section: Methodsmentioning

confidence: 99%

“…The following changes were made in relation to the methodology described previously (16). Sprouts were excised when they were 6 to 8 cm long.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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